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Usp30 (NM_001033202) Mouse Tagged ORF Clone
CAT＃: MR226441
- TrueORF®
Usp30 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30)
ORF Plasmid: tGFP
"NM_001033202" in other vectors (4)
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CNY 3,848.00
CNY 4,470.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6330590F17Rik; AI851327; D5Ertd483e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226441 representing NM_001033202
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAAGTTCCCGGGCGCAGGCGGCGAGGACGGCGGCCGACAAGGCCCTGCAGCGCTTCCTGCGCACCG GGGCGGCCGTCAGATACAAAGTCATGAAGAACTGGGGAGTGATCGGTGGGATTGCCGCTGCCCTGGCAGC AGGGATCTATGTTATTTGGGGTCCCATTACAGAGAGGAAGAAGCGGAGAAAAGGGCTTGTGCCTGGCCTT GTCAATCTGGGGAATACCTGCTTCATGAACTCGCTGCTGCAGGGCCTGTCTGCCTGCCCTGCGTTTGTCA AGTGGCTGGAAGAGTTTACCACCCAGTACTCCAGGGACCAGCAGGGGCCACACACTCACCAGTGCCTGTC CTTAACACTGCTGAACCTCCTGAAAGCTCTGTCCTGCCAAGAAGTCACCGAGGACGAGGTCTTGGACGCC AGCTGTCTGCTGGACGTCCTGAGAATGTACAGATGGCAGATCTCGTCCTTTGAGGAGCAGGATGCTCACG AGCTGTTTCATGTCATCACCTCATCACTGGAGGACGAGAGAGACCGCCAGCCTCGAGTCACCCACTTGTT TGATGTGCATTCCCTGGAGCAGCAGTCAGAAATGGCTCCCAGACAAGTCACTTGCCACACGAGAGGGTCC CCTCACCCCACGACCAATCACTGGAAGTCTCAGCACCCTTTCCATGGAAGACTCACCAGTAACATGGTGT GCAAACACTGTGAACACCAGAGTCCTGTTCGGTTTGACACCTTTGACAGCCTTTCTCTCAGTATTCCAGC TGCCACTTGGGGGCACCCACTGACACTGGACCACTGCCTTCACCACTTCATCTCATCAGAGTCCGTGCGA GACGTTGTGTGTGACAACTGTACAAAGATTGAAGCGAGGGGGACACTGACCGGGGAGAAGGTGGAGCATC AGAGGAGCACCTTTGTTAAACAGTTAAAACTCGGAAAGCTGCCACAGTGCCTCTGCATCCACCTGCAGCG GCTCAGCTGGTCCAGCCACGGCACGCCCTTGAAGCGGCACGAGCACGTGCAGTTTAACGAGTTCCTGATG ATGGACTTCTACAAGTACCGCCTCCTGGGACACAAGCCCAGCCAGCATGGCCCCAAAGCCACCGAGAACC CAGGGTCTGCCCCAGAAGTACAGGACGCACAGGCGGCCCCAAAGCCAGGTCTGAGTCAGCCAGGGGCCCC CAAGACACAGATTTTTCTGAATGGCGCCTGCTCCCCGTCTCTGTTGCCAGCCCTGCCTTCCCCGGTGGCC TTCCCTCTGCCAGTGGTTCCTGACTACAGCTCCTCCACATACCTCTTCCGCTTGATGGCAGTTGTCGTTC ACCATGGAGACATGCACTCTGGACACTTTGTCACCTACAGACGGTCACCACCTTCTGCCAAGAACCCTCT CTCAACCAGCAACCAATGGCTGTGGATTTCTGACGACACCGTCCGCAAGGCCAGCCTGCAGGAGGTCCTG TCCTCTAGTGCCTACCTGCTGTTCTATGAGCGAGTTCTGTCCAGGGTGCAGCAGCAGGGGCGAGAGTATA GGTCTGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226441 representing NM_001033202
Red=Cloning site Green=Tags(s) MLSSRAQAARTAADKALQRFLRTGAAVRYKVMKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPGL VNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLNLLKALSCQEVTEDEVLDA SCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDRQPRVTHLFDVHSLEQQSEMAPRQVTCHTRGS PHPTTNHWKSQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVR DVVCDNCTKIEARGTLTGEKVEHQRSTFVKQLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLM MDFYKYRLLGHKPSQHGPKATENPGSAPEVQDAQAAPKPGLSQPGAPKTQIFLNGACSPSLLPALPSPVA FPLPVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSAKNPLSTSNQWLWISDDTVRKASLQEVL SSSAYLLFYERVLSRVQQQGREYRSEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033202 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033202.3, NP_001028374.1 |
| RefSeq Size | 2790 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 100756 |
| UniProt ID | Q3UN04 |
| MW | 58.7 kDa |
| Gene Summary | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (By similarity). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (PubMed:24513856).[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Parkin interacts with Mitofilin to increase dopaminergic neuron death in response to Parkinson\'s disease-related stressors
,Imam Aliagan, AD;Ahwazi, MD;Tombo, N;Feng, Y;Bopassa, JC;,
American journal of translational research
,PubMed ID 33312388
[USP30]
|
|
Dual role of USP30 in controlling basal pexophagy and mitophagy
,Marcassa, E;Kallinos, A;Jardine, J;Rusilowicz-Jones, EV;Martinez, A;Kuehl, S;Islinger, M;Clague, MJ;Urbé, S;,
EMBO Rep.
,PubMed ID 29895712
[USP30]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217339 | Usp30 (untagged) - Mouse ubiquitin specific peptidase 30 (Usp30), (10ug) |
CNY 6,650.00 |
|
| MG226441 | Usp30 (tGFP-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30), (10ug) |
CNY 5,448.00 |
|
| MR226441L3 | Lenti ORF clone of Usp30 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30) |
CNY 6,370.00 |
|
| MR226441L4 | Lenti ORF clone of Usp30 (mGFP-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30) |
CNY 6,370.00 |
