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Lipe (NM_010719) Mouse Tagged ORF Clone
CAT＃: MR226805
- TrueORF®
Lipe (Myc-DDK-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 1
ORF Plasmid: tGFP
"NM_010719" in other vectors (3)
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CNY 6,464.00
CNY 7,980.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4933403G17Rik; HSL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226805 representing NM_010719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGCCGTGGAATCGGCGCCCGTGGGGGCCCAGGCCTCCAAGCAGGGCAAAGAAGGATCGAAGA ACCGCAGTCGCAGGCGGTGGCGAAAAGGCAAGATCAAAGCCTCAGCGTTCTCACACAGCATGGATTTACG CACGATGACACAGTCGCTGGTGACACTCGCAGAAGACAATATGGCCTTCTTCTCAAGCCAGGGCCCAGGA GAGACAGCACGGCGGCTGTCTAATGTCTTTGCAGGTGTTCGGGAACAGGCACTGGGGCTGGAACCAACCC TAGGCCAACTGTTGGGTGTGGCACACCATTTTGACCTGGACACAGAGACACCAGCCAACGGATACCGTAG TTTGGTGCACACAGCCCGATGCTGCCTGGCACACCTACTACACAAATCCCGCTATGTGGCTTCTAACCGC AAAAGTATCTTCTTCCGTGCCAGCCACAACCTAGCAGAGCTGGAGGCCTACCTGGCCGCCCTCACCCAGC TCCGTGCTATGGCCTACTATGCCCAGCGCCTGCTGACCATCAACCGACCAGGAGTGCTCTTCTTCGAGGG TGATGAAGGACTCACCGCTGACTTCCTGCAAGAGTATGTCACGCTACACAAAGGCTGCTTCTACGGCCGC TGCCTGGGCTTCCAGTTCACACCTGCCATCCGGCCGTTCCTGCAGACTCTCTCCATCGGGCTGGTGTCCT TCGGGGAGCACTACAAACGCAACGAGACAGGCCTCAGTGTGACCGCCAGTTCCCTCTTTACCGGTGGCCG ATTCGCCATAGACCCAGAGTTGCGTGGGGCTGAATTTGAACGCATCATACAGAACCTGGATGTGCACTTC TGGAAAGCCTTCTGGAACATCACTGAGATTGAGGTGCTGTCGTCTCTGGCCAACATGGCATCAACCACTG TGAGGGTAAGCCGCCTGCTCAGCTTGCCACCTGAGGCCTTTGAGATGCCACTCACCTCTGATCCCAGGCT CACAGTTACCATCTCACCTCCCTTGGCACACACGGGACCAGCTCCTGTGCTAGCCAGGCTCATCTCCTAT GACCTACGGGAAGGACAGGACAGCAAGGTACTCAACAGCCTGGCAAAATCTGAGGGCCCACGCCTGGAGC TGCGCCCACGGCCTCACCAAGCACCCCGTTCACGGGCCCTGGTTGTTCACATCCACGGAGGCGGCTTTGT GGCACAGACCTCTAAATCCCACGAGCCCTACCTCAAGAACTGGGCCCAGGAGCTAGGAGTCCCTATCTTC TCCATCGACTACTCCCTGGCCCCCGAGGCTCCCTTTCCCCGAGCGCTGGAGGAGTGTTTTTTTGCCTACT GCTGGGCTGTCAAGCACTGTGACCTGCTTGGTTCAACTGGAGAGCGGATATGCCTTGCAGGGGACAGTGC AGGTGGGAATCTCTGCATCACTGTGTCCCTTCGGGCAGCAGCCTATGGAGTGAGGGTGCCAGATGGCATC ATGGCAGCCTACCCAGTTACCACCCTGCAGTCCTCTGCTTCTCCCTCTCGTCTGCTGAGCCTCATGGACC CTCTTCTACCACTGAGCGTACTCTCTAAGTGTGTCAGTGCCTATTCAGGGACAGAGGCAGAGGACCATTT TGACTCAGACCAGAAGGCACTAGGCGTGATGGGGCTGGTGCAGAGAGACACTTCGCTGTTCCTCAGAGAC CTCCGACTGGGTGCCTCCTCATGGCTCAACTCCTTCCTGGAACTAAGTGGACGCAAGCCCCAAAAGACCA CATCGCCCACAGCAGAGTCTGTGCGCCCCACGGAGTCTATGCGCAGGAGTGTGTCTGAGGCAGCCCTGGC CCAGCCTGAGGGCTTACTGGGCACAGATACCTTGAAGAAGCTGACAATAAAGGACTTGAGCAACTCAGAG CCTTCAGACAGCCCCGAGATGTCACAGTCAATGGAGACACTTGGCCCCTCCACACCCTCTGATGTCAACT TTTTTCTGCGGCCTGGGAATTCCCAGGAAGAGGCTGAAGCCAAAGATGAAGTGAGACCCATGGACGGAGT CCCCCGCGTGCGCGCTGCTTTCCCTGAGGGGTTTCACCCCCGGCGCTCAAGCCAAGGTGTCCTCCACATG CCCCTCTACACGTCACCCATAGTCAAGAACCCCTTCATGTCTCCTCTGCTGGCCCCTGACAGCATGCTGA AGACCTTGCCGCCTGTGCACCTTGTGGCTTGCGCTCTGGACCCCATGCTAGATGACTCGGTCATGTTCGC GCGGCGACTGCGCGACCTGGGCCAGCCGGTGACGCTGAAAGTGGTAGAAGATCTGCCGCATGGCTTCCTG AGCCTGGCGGCACTGTGTCGCGAGACCCGGCAGGCCACGGAGTTCTGCGTGCAGCGCATCCGGCTGATCC TCACCCCGCCTGCTGCACCACTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226805 representing NM_010719
Red=Cloning site Green=Tags(s) MEPAVESAPVGAQASKQGKEGSKNRSRRRWRKGKIKASAFSHSMDLRTMTQSLVTLAEDNMAFFSSQGPG ETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNR KSIFFRASHNLAELEAYLAALTQLRAMAYYAQRLLTINRPGVLFFEGDEGLTADFLQEYVTLHKGCFYGR CLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLFTGGRFAIDPELRGAEFERIIQNLDVHF WKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTSDPRLTVTISPPLAHTGPAPVLARLISY DLREGQDSKVLNSLAKSEGPRLELRPRPHQAPRSRALVVHIHGGGFVAQTSKSHEPYLKNWAQELGVPIF SIDYSLAPEAPFPRALEECFFAYCWAVKHCDLLGSTGERICLAGDSAGGNLCITVSLRAAAYGVRVPDGI MAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTEAEDHFDSDQKALGVMGLVQRDTSLFLRD LRLGASSWLNSFLELSGRKPQKTTSPTAESVRPTESMRRSVSEAALAQPEGLLGTDTLKKLTIKDLSNSE PSDSPEMSQSMETLGPSTPSDVNFFLRPGNSQEEAEAKDEVRPMDGVPRVRAAFPEGFHPRRSSQGVLHM PLYTSPIVKNPFMSPLLAPDSMLKTLPPVHLVACALDPMLDDSVMFARRLRDLGQPVTLKVVEDLPHGFL SLAALCRETRQATEFCVQRIRLILTPPAAPLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010719 |
| ORF Size | 2406 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010719.5, NP_034849.2 |
| RefSeq Size | 3221 bp |
| RefSeq ORF | 2409 bp |
| Locus ID | 16890 |
| UniProt ID | P54310 |
| MW | 88.5 kDa |
| Gene Summary | In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226805 | Lipe (tGFP-tagged) - Mouse lipase hormone sensitive (Lipe) transcript variant 1, (10ug) |
CNY 8,740.00 |
|
| MR226805L3 | Lenti ORF clone of Lipe (Myc-DDK-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 1 |
CNY 8,864.00 |
|
| MR226805L4 | Lenti ORF clone of Lipe (mGFP-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 1 |
CNY 9,880.00 |
