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Gata1 (NM_008089) Mouse Tagged ORF Clone
CAT＃: MR226904
- TrueORF®
Gata1 (Myc-DDK-tagged) - Mouse GATA binding protein 1 (Gata1)
ORF Plasmid: tGFP
"NM_008089" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | eryf1; Gata-1; Gf-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226904 representing NM_008089
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTTCCTGGTCTAGGGGCCCTGGGGACCTCAGAACCCTTGCCCCAGTTTGTGGATTCTGCCCTGG TGTCCTCACCATCAGATTCCACAGGTTTCTTTTCCTCTGGGCCAGAGGGTTTGGATGCAGCATCTTCTTC CACTTCCCCAAATGCAGCCACGGCCGCAGCATCAGCACTGGCCTACTACAGAGAAGCTGAGGCCTACAGA CACTCCCCAGTCTTTCAGGTGTATCCACTGCTCAACAGTATGGAGGGAATTCCTGGGGGCTCACCTTATG CTAGCTGGGCCTATGGCAAGACGGCACTCTACCCTGCCTCAACTGTGTGCCCCAGCCATGAGGATGCCCC TTCCCAGGCCCTGGAAGACCAGGAAGGGAAGAGCAACAACACGTTCTTGGACACCTTGAAGACGGAGCGG CTGAGTCCAGACCTCCTGACGCTGGGGACTGCACTGCCTGCATCGCTCCCTGTCACCGGCAGTGCTTACG GGGGAGCTGACTTTCCCAGTCCTTTCTTCTCTCCCACTGGGAGCCCTCTCAGCTCAGCAGCCTATTCTTC CCCCAAGTTTCATGGAAGCCTGCCATTGGCCCCTTGTGAGGCCAGAGAGTGTGTGAACTGTGGAGCAACG GCTACTCCACTGTGGCGGAGGGACAGGACAGGTCACTACCTGTGCAATGCCTGTGGCTTGTATCACAAGA TGAATGGTCAGAACCGGCCTCTCATCCGGCCCAAGAAGCGAATGATTGTCAGCAAACGGGCAGGCACCCA ATGCACTAACTGTCAAACGACCACTACAACACTCTGGCGGAGGAATGCCAGCGGAGATCCGGTATGCAAT GCCTGCGGCCTCTATTTCAAGCTCCATCAGGTGAACCGCCCACTGACCATGAGGAAAGATGGAATCCAGA CGAGGAACCGCAAGGCATCTGGCAAAGGGAAAAAGAAGCGGGGGTCGAATCTGGCTGGAGCGGGAGCGGC TGAAGGACCAGCTGGTGGCTTCATGGTGGTAGCTGGTAGCAGCAGTAGTGGGAATTGTGGGGAGGTGGCC TCAGGCTTGGCACTGGGCACTGCAGGTACTGCCCACCTCTATCAGGGCCTAGGACCTGTGGTACTGTCAG GGCCTGTCAGCCATCTTATGCCTTTTCCTGGACCTCTGCTGGGATCGCCTACAACCTCCTTCCCCACCGG TCCTGCACCTACCACCAGCAGCACCAGCGTGATCGCCCCACTCAGTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226904 representing NM_008089
Red=Cloning site Green=Tags(s) MDFPGLGALGTSEPLPQFVDSALVSSPSDSTGFFSSGPEGLDAASSSTSPNAATAAASALAYYREAEAYR HSPVFQVYPLLNSMEGIPGGSPYASWAYGKTALYPASTVCPSHEDAPSQALEDQEGKSNNTFLDTLKTER LSPDLLTLGTALPASLPVTGSAYGGADFPSPFFSPTGSPLSSAAYSSPKFHGSLPLAPCEARECVNCGAT ATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRMIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCN ACGLYFKLHQVNRPLTMRKDGIQTRNRKASGKGKKKRGSNLAGAGAAEGPAGGFMVVAGSSSSGNCGEVA SGLALGTAGTAHLYQGLGPVVLSGPVSHLMPFPGPLLGSPTTSFPTGPAPTTSSTSVIAPLSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008089 |
| ORF Size | 1242 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008089.2, NP_032115.1 |
| RefSeq Size | 1902 bp |
| RefSeq ORF | 1242 bp |
| Locus ID | 14460 |
| UniProt ID | P17679 |
| MW | 42.7 kDa |
| Gene Summary | Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208526 | Gata1 (untagged) - Mouse GATA binding protein 1 (Gata1), (10ug) |
CNY 5,856.00 |
|
| MG226904 | Gata1 (tGFP-tagged) - Mouse GATA binding protein 1 (Gata1), (10ug) |
CNY 4,470.00 |
|
| MR226904L3 | Lenti ORF clone of Gata1 (Myc-DDK-tagged) - Mouse GATA binding protein 1 (Gata1) |
CNY 5,990.00 |
|
| MR226904L4 | Lenti ORF clone of Gata1 (mGFP-tagged) - Mouse GATA binding protein 1 (Gata1) |
CNY 5,990.00 |
