Upf2 (NM_001081132) Mouse Tagged Lenti ORF Clone
CAT#: MR226926L4
- LentiORF®
Lenti ORF clone of Upf2 (mGFP-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2). Note: ORF is codon optimized
"NM_001081132" in other vectors (4)
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CNY 14,540.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR226926).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001081132 |
ORF Size | 3807 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001081132.1, NP_001074601.1 |
RefSeq Size | 5174 bp |
RefSeq ORF | 3810 bp |
Locus ID | 326622 |
UniProt ID | A2AT37 |
Gene Summary | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224119 | Upf2 (untagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), (10ug) |
CNY 16,340.00 |
|
MG226926 | Upf2 (tGFP-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), (10ug). Note: ORF is codon optimized |
CNY 13,870.00 |
|
MR226926 | Upf2 (Myc-DDK-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2). Note: ORF is codon optimized |
CNY 8,840.00 |
|
MR226926L3 | Lenti ORF clone of Upf2 (Myc-DDK-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2). Note: ORF is codon optimized |
CNY 14,540.00 |