Itgb4 (NM_001005608) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA407042; C230078O20; CD104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227152 representing NM_001005608 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGCCCTGTTGCAGCCCATGGGTGAAGCTGCTGCTGCTGGCAGCAATGCTGAGTGCCAGCCTCC CTGGAGACCTGGCCAACCGCTGCAAGAAGGCTCAGGTGAAGAGCTGTACCGAGTGCATCCGGGTGGACAA GAGCTGTGCCTACTGCACAGACGAGCTGTTCAAGGAGAGGCGCTGCAACACCCAGGCGGAGCTTCTGGCT GCAGGCTGCAGGGGAGAGAGCATCCTGGTCATGGAGAGCAGCCTTGAAATCACAGAGAACACCCAGATCG ACACCAGCCTGCACCGCAGCCAGGTATCTCCCCAAGGCCTGCAAGTCCGGCTGCGGCCGGGTGAGGAGCG CAGCTTTGTGTTCCAGGTGTTTGAGCCCCTGGAGAGCCCCGTGGATCTGTATATCCTCATGGACTTCTCC AACTCCATGTCTGACGATCTGGACAACCTCAAGCAGATGGGGCAGAACCTGGCCAAGATCCTGCGCCAGC TCACCAGCGACTACACCATTGGATTTGGAAAGTTTGTGGACAAAGTCAGCGTCCCACAGACAGACATGAG GCCCGAGAAACTGAAGGAGCCCTGGCCCAACAGTGATCCCCCGTTCTCCTTCAAGAACGTTATCAGCTTA ACGGAGAATGTGGAAGAATTCTGGAACAAACTGCAAGGAGAACGCATCTCAGGCAACCTGGACGCTCCTG AAGGGGGCTTTGATGCCATCCTGCAGACAGCTGTGTGCACAAGGGACATTGGCTGGAGGGCTGACAGCAC CCACCTGCTGGTGTTCTCCACCGAGTCTGCCTTCCACTACGAGGCTGATGGTGCCAACGTGCTGGCCGGC ATCATGAACCGCAATGATGAGAAATGCCACCTGGACGCCTCGGGCGCCTACACCCAATACAAGACACAGG ACTACCCATCAGTGCCCACGCTGGTTCGCCTGCTTGCCAAGCATAACATCATCCCCATCTTTGCTGTCAC CAACTACTCTTACAGCTACTATGAGAAGCTCCATAAGTATTTCCCCGTCTCCTCTCTGGGCGTCCTGCAG GAGGATTCATCCAACATCGTGGAGCTGCTGGAGGAGGCCTTCTATCGAATTCGCTCCAACCTGGACATCC GGGCTCTGGACAGCCCCAGAGGCCTGAGAACAGAGGTCACCTCCGATACTCTCCAGAAGACGGAGACTGG GTCCTTTCACATCAAGCGGGGGGAAGTGGGCACATACAATGTGCATCTCCGGGCAGTGGAGGACATAGAT GGGACACATGTGTGCCAGCTGGCTAAAGAAGACCAAGGGGGCAACATCCACCTGAAACCCTCCTTCTCTG ATGGCCTCCGGATGGACGCGAGTGTGATCTGTGACGTGTGCCCCTGTGAGCTGCAAAAGGAAGTTCGATC AGCTCGCTGTCACTTCAGAGGAGACTTCATGTGTGGACACTGTGTGTGCAATGAGGGCTGGAGTGGCAAA ACCTGCAACTGCTCCACCGGCTCTCTGAGTGACACACAGCCCTGCCTGCGTGAGGGTGAGGACAAACCGT GCTCGGGCCACGGCGAGTGCCAGTGCGGACGCTGTGTGTGCTATGGTGAAGGCCGCTACGAGGGTCACTT CTGCGAGTATGACAACTTCCAGTGTCCCCGGACCTCTGGATTCCTGTGCAATGACCGGGGTCGCTGTTCT ATGGGAGAGTGTGTGTGTGAGCCTGGTTGGACAGGCCGCAGCTGTGACTGTCCCCTCAGCAATGCCACCT GCATCGATAGCAACGGGGGCATCTGCAACGGCCGAGGCTACTGTGAGTGTGGCCGTTGTCACTGCAACCA GCAGTCGCTCTACACGGACACCACCTGTGAGATCAACTACTCTGCGATACGCCTGGGTCTCTGTGAGGAT CTCCGCTCCTGCGTACAGTGCCAGGCCTGGGGCACCGGGGAGAAGAAAGGGCGCGCGTGTGACGATTGCC CCTTTAAAGTCAAGATGGTAGACGAGCTTAAGAAAGAAGAGGTGGTGGAGTACTGCTCCTTCCGGGATGA GGATGACGACTGCACTTACAGCTACAACGTGGAGGGCGATGGCAGCCCTGGGCCCAACAGCACAGTCCTG GTCCACAAAAAGAAAGACTGCCCTCCCGGCTCCTTCTGGTGGCTCATCCCCCTGCTCATCTTCCTCCTGT TGCTCCTGGCGTTGCTTCTGCTGCTCTGCTGGAAATACTGTGCCTGCTGCAAAGCCTGCCTGGGGCTTCT TCCTTGCTGCAACCGAGGTCACATGGTGGGCTTTAAGGAAGATCACTATATGCTTCGGGAGAACCTGATG GCCTCTGACCACCTGGACACGCCCATGCTACGAAGCGGGAACCTCAAGGGACGAGACACAGTCCGCTGGA AGATCACCAACAATGTGCAGCGCCCTGGCTTTGCCACCCATGCCGCCAGCACCAGCCCCACGGAGCTCGT ACCCTACGGGCTGTCCCTGCGCCTTGGCCGCCTCTGCACTGAGAACCTTATGAAGCCGGGCACCCGAGAG TGTGACCAGCTACGCCAGGAGGTGGAGGAAAATCTGAATGAGGTGTATAGACAGGTCAGCGGCGCACACA AGCTCCAGCAGACGAAGTTCCGACAGCAGCCCAACACCGGGAAAAAGCAAGACCACACCATTGTGGACAC AGTGTTGCTGGCGCCCCGCTCCGCCAAGCAGATGCTGCTGAAGCTGACAGAGAAGCAGGTGGAGCAGGGG TCCTTCCATGAACTGAAGGTGGCCCCTGGCTACTACACTGTCACGGCAGAGCAGGATGCCCGGGGCATGG TGGAGTTCCAGGAGGGCGTGGAGCTGGTGGATGTGCGAGTGCCCCTCTTCATCCGGCCTGAGGATGATGA TGAGAAGCAGCTGCTGGTGGAGGCCATTGATGTCCCTGTGGGCACTGCCACCCTTGGTCGCCGTCTGGTA AACATCACCATTATCAAGGAACAAGCTAGTGGGGTAGTGTCCTTCGAGCAGCCTGAATACTCGGTGAGTC GTGGAGACCAGGTGGCCCGCATCCCTGTCATCCGGCACATCCTGGACAATGGCAAGTCCCAGGTCTCCTA TAGCACACAGGATAATACAGCACACGGACACCGGGATTATGTCCCTGTGGAGGGAGAGCTGCTGTTCCAT CCTGGGGAGACCTGGAAGGAGTTGCAGGTGAAGCTACTGGAGCTGCAGGAGGTCGACTCCCTCCTGCGTG GCCGCCAGGTCCGCCGCTTCCAAGTCCAACTCAGCAACCCCAAGTTCGGAGCCCGCCTGGGCCAGCCCAG CACAACCACCGTTATTCTCGATGAAACGGACAGGAGTCTCATAAATCAAACACTTTCATCGCCTCCGCCA CCCCATGGAGACCTGGGCGCCCCACAGAACCCCAATGCCAAGGCTGCCGGGTCCAGGAAGATCCATTTCA ACTGGCTGCCCCCTCCTGGCAAGCCAATGGGGTACAGGGTGAAGTACTGGATCCAGGGCGACTCTGAGTC TGAAGCCCACCTTCTAGACAGCAAGGTGCCCTCAGTGGAACTCACCAACCTGTATCCCTATTGCGACTAC GAAATGAAGGTGTGTGCCTATGGGGCACAGGGTGAGGGGCCCTATAGCTCACTGGTGTCCTGCCGCACCC ACCAGGAAGTACCCAGCGAGCCAGGGAGGCTGGCTTTCAATGTAGTCTCTTCTACGGTGACGCAGCTGAG CTGGGCAGAGCCAGCTGAGACCAATGGCGAGATCACAGCCTACGAGGTCTGCTATGGACTGGTCAATGAG GACAACCGACCCATTGGACCTATGAAGAAGGTGCTCGTGGACAACCCCAAGAACCGGATGCTGCTCATTG AGAATCTGCGAGAATCCCAGCCATACCGATACACGGTTAAGGCGCGCAATGGGGCAGGATGGGGACCCGA GAGAGAGGCTATCATCAACCTGGCTACACAGCCCAAGCGGCCCATGTCCATCCCTATCATCCCAGACATC CCCATAGTGGACGCCCAGGGTGGAGAAGACTACGAAAACTTCCTTATGTACAGTGATGACGTCCTGCGGT CCCCAGCCAGCAGCCAGAGGCCCAGCGTTTCTGATGACACTGAGCACCTGGTGAATGGCCGGATGGACTT TGCCTATCCAGGCAGCGCCAACTCCCTGCACAGAATGACTGCAGCCAATGTGGCCTATGGCACGCATCTG AGCCCACACCTGTCCCACCGAGTGCTGAGCACGTCCTCCACCCTTACCCGGGACTACCACTCTCTGACAC GCACAGAGCACTCCCACTCAGGCACACTTCCCAGGGACTACTCCACCCTCACTTCCCTTTCCTCCCAAGG CCTCCCTCCTATCTGGGAAGATGGGAGGAGCAGGCTTCCGCTGTCCTGGACTCTTGGGTCCTTGAGCCGG GCTCACATGAAGGGTGTGCCCGCATCCAGGGGTTCACCAGACTCTATAATCCTGGCCGGGCAGTCAGCAG CACCCTCCTGGGGTACAGATTCCCGTGGGGCTGTGGGTGTGCCTGACACACCCACTCGGCTGGTGTTCTC TGCCCTGGGGCCCACGTCTTTGAAGGTGAGCTGGCAGGAGCCACAGTGTGATCGGATGCTGCTGGGCTAC AGTGTGGAATACCAGCTACTAAACGGCGGCGAGATGCATCGGCTCAACATCCCTAACCCTGGCCAAACCT CGGTGGTGGTAGAGGATCTCCTGCCTAACCACTCCTATGTGTTCCGGGTACGGGCCCAGAGCCAGGAGGG CTGGGGCCGAGAGCGAGAGGGTGTCATCACCATCGAGTCCCAGGTGCACCCGCAGAGCCCTCTCTGCCCC CTGCCAGGCTCAGCCTTCACTCTGAGCACCCCCAGCGCCCCGGGACCGCTGGTGTTCACTGCCCTAAGCC CAGACTCCCTGCAGCTCAGCTGGGAGCGGCCGAGGAGGCCCAATGGAGATATCCTTGGCTACCTGGTGAC CTGTGAGATGGCCCAAGGAGGAGCACCAGCCAGGACCTTCCGGGTGGACGGAGACAACCCTGAGAGCCGG TTGACTGTACCTGGCCTCAGTGAGAACGTTCCTTACAAGTTCAAGGTTCAGGCCAGGACGACCGAGGGCT TCGGGCCAGAGCGTGAGGGTATCATCACCATCGAGTCTCAGGTTGGAGGCCCCTTCCCACAGCTGGGCAG CCATTCTGGGCTCTTCCAGAACCCAGTGCAAAGCGAGTTCAGCAGCGTGACCAGCACGCACAGCACCACG ACTGAGCCCTTCCTCATGGATGGTCTAACCCTGGGGACCCAGCGCCTGGAAGCAGGAGGCTCCCTCACCC GGCATGTGACCCAGGAATTCGTGACCCGGACCTTAACGGCCAGTGGCTCTCTCAGCACTCATATGGACCA ACAGTTCTTCCAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227152 representing NM_001005608 Red=Cloning site Green=Tags(s) MAGPCCSPWVKLLLLAAMLSASLPGDLANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQAELLA AGCRGESILVMESSLEITENTQIDTSLHRSQVSPQGLQVRLRPGEERSFVFQVFEPLESPVDLYILMDFS NSMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISL TENVEEFWNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAG IMNRNDEKCHLDASGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQ EDSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDID GTHVCQLAKEDQGGNIHLKPSFSDGLRMDASVICDVCPCELQKEVRSARCHFRGDFMCGHCVCNEGWSGK TCNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCS MGECVCEPGWTGRSCDCPLSNATCIDSNGGICNGRGYCECGRCHCNQQSLYTDTTCEINYSAIRLGLCED LRSCVQCQAWGTGEKKGRACDDCPFKVKMVDELKKEEVVEYCSFRDEDDDCTYSYNVEGDGSPGPNSTVL VHKKKDCPPGSFWWLIPLLIFLLLLLALLLLLCWKYCACCKACLGLLPCCNRGHMVGFKEDHYMLRENLM ASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASTSPTELVPYGLSLRLGRLCTENLMKPGTRE CDQLRQEVEENLNEVYRQVSGAHKLQQTKFRQQPNTGKKQDHTIVDTVLLAPRSAKQMLLKLTEKQVEQG SFHELKVAPGYYTVTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRLV NITIIKEQASGVVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLFH PGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPSTTTVILDETDRSLINQTLSSPPP PHGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDY EMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNE DNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDI PIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAYGTHL SPHLSHRVLSTSSTLTRDYHSLTRTEHSHSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLGSLSR AHMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAVGVPDTPTRLVFSALGPTSLKVSWQEPQCDRMLLGY SVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCP LPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGAPARTFRVDGDNPESR LTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQVGGPFPQLGSHSGLFQNPVQSEFSSVTSTHSTT TEPFLMDGLTLGTQRLEAGGSLTRHVTQEFVTRTLTASGSLSTHMDQQFFQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001005608
ORF Size	5406 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001005608.2, NP_001005608.2
RefSeq Size	6160 bp
RefSeq ORF	5409 bp
Locus ID	192897
MW	200.3 kDa
Gene Summary	Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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