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Fbxo32 (NM_026346) Mouse Tagged ORF Clone
CAT＃: MR227260
- TrueORF®
Fbxo32 (Myc-DDK-tagged) - Mouse F-box protein 32 (Fbxo32)
ORF Plasmid: tGFP
"NM_026346" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4833442G10Rik; AI430017; ATROGIN1; Gm20361; MAFbx |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227260 representing NM_026346
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTTCCTTGGGCAGGACTGGCGGTCCCCGGGCCAGAGCTGGGTGAAGACGGCGGACGGCTGGAAGC GCTTCTTGGATGAGAAAAGCGGCAGCTTCGTGAGCGACCTCAGCAGTTACTGCAACAAGGAGGTATACAG TAAGGAGAATCTGTTCAGCAGCCTGAACTACGACGTCGCAGCCAAGAAGAGAAAGAAAGACATTCAGAAC AGCAAAACCAAAACTCAGTACTTCCATCAAGAAAAGTGGATCTATGTTCACAAAGGAAGTACGAAGGAGC GCCATGGATACTGTACTTTGGGGGAAGCTTTCAACAGACTGGACTTCTCGACTGCCATCCTGGATTCCAG AAGATTCAACTACGTAGTAAGGCTGTTGGAGCTGATAGCAAAGTCACAGCTCACATCCCTGAGTGGCATC GCCCAAAAGAACTTCATGAACATTTTGGAAAAAGTGGTACTGAAAGTTCTTGAAGACCAGCAAAACATAA GACTTATACGGGAACTTCTCCAGACTCTCTACACATCCTTATGCACACTGGTGCAGAGAGTCGGCAAGTC TGTGCTGGTGGGCAACATTAACATGTGGGTGTATCGGATGGAGACCATTCTACACTGGCAGCAGCAGCTG AATAGCATCCAGATCAGCAGGCCTGCCTTCAAAGGCCTCACGATCACCGACCTGCCTGTGTGCTTACAAC TGAACATCATGCAGAGGCTGAGTGACGGGCGGGACCTGGTCAGCCTGGGCCAGGCAGCCCCAGACCTGCA TGTGCTCAGTGAGGACCGGCTACTGTGGAAGAGACTCTGCCAGTACCACTTCTCAGAGAGGCAGATTCGC AAGCGTTTGATCTTGTCTGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTTAAGCTTGTACGATGTT ACCCAAGAAGAGAGCAGTATGGGGTCACCCTGCAGCTTTGCAAACACTGCCACATTCTCTCCTGGAAGGG CACTGACCATCCGTGCACGGCCAACAACCCAGAGAGCTGCTCCGTCTCACTTTCCCCTCAAGACTTTATC AATTTGTTCAAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227260 representing NM_026346
Red=Cloning site Green=Tags(s) MPFLGQDWRSPGQSWVKTADGWKRFLDEKSGSFVSDLSSYCNKEVYSKENLFSSLNYDVAAKKRKKDIQN SKTKTQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSTAILDSRRFNYVVRLLELIAKSQLTSLSGI AQKNFMNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETILHWQQQL NSIQISRPAFKGLTITDLPVCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKRLCQYHFSERQIR KRLILSDKGQLDWKKMYFKLVRCYPRREQYGVTLQLCKHCHILSWKGTDHPCTANNPESCSVSLSPQDFI NLFKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026346 |
| ORF Size | 1065 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026346.3, NP_080622.1 |
| RefSeq Size | 6936 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 67731 |
| UniProt ID | Q9CPU7 |
| MW | 42 kDa |
| Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210615 | Fbxo32 (untagged) - Mouse F-box protein 32 (Fbxo32), (10ug) |
CNY 3,990.00 |
|
| MG227260 | Fbxo32 (tGFP-tagged) - Mouse F-box protein 32 (Fbxo32), (10ug) |
CNY 7,088.00 |
|
| MR227260L3 | Lenti ORF clone of Fbxo32 (Myc-DDK-tagged) - Mouse F-box protein 32 (Fbxo32) |
CNY 5,890.00 |
|
| MR227260L4 | Lenti ORF clone of Fbxo32 (mGFP-tagged) - Mouse F-box protein 32 (Fbxo32) |
CNY 5,890.00 |
