Itgam (NM_001082960) Mouse Tagged ORF Clone
CAT#: MR227326
- TrueORF®
Itgam (Myc-DDK-tagged) - Mouse integrin alpha M (Itgam), transcript variant 1
ORF Plasmid: tGFP
"NM_001082960" in other vectors (4)
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CNY 12,064.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227326 representing NM_001082960
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTTAAAGCTCTTCTGGTCACAGCCCTAGCCTTGTGTCATGGCTTCAATCTGGACACTGAACATC CCATGACCTTCCAAGAGAATGCAAAAGGCTTTGGACAGAGTGTGGTCCAGCTTGGCGGAACCAGTGTGGT TGTTGCAGCCCCCCAGGAGGCAAAGGCTGTTAACCAGACAGGTGCCCTCTACCAGTGTGACTACAGCACA AGCCGGTGTCACCCCATCCCCCTGCAAGTACCTCCAGAGGCTGTGAATATGTCCTTGGGCCTGTCCCTGG CTGTTTCTACTGTCCCCCAGCAGCTGCTGGCCTGTGGCCCCACGGTGCACCAAAACTGCAAGGAGAATAC TTATGTGAATGGATTGTGCTATTTGTTCGGCTCCAACCTGCTGAGGCCGCCCCAGCAGTTCCCAGAGGCT CTCAGAGAATGTCCTCAGCAGGAGAGTGACATTGTCTTCTTGATTGATGGCTCCGGTAGCATCAACAACA TTGACTTTCAGAAGATGAAGGAGTTTGTCTCAACTGTGATGGAGCAGTTCAAAAAGTCTAAAACCTTGTT CTCTTTGATGCAGTACTCGGACGAGTTCCGGATTCACTTCACCTTCAATGACTTCAAGAGAAACCCTAGC CCAAGATCACATGTGAGCCCCATAAAGCAGCTGAATGGGAGGACAAAAACTGCCTCAGGGATCCGGAAAG TAGTGAGAGAACTGTTTCACAAAACCAATGGGGCCCGGGAGAATGCTGCGAAGATCCTAGTTGTCATCAC AGATGGAGAAAAATTCGGTGATCCCTTGGATTATAAGGATGTCATCCCCGAGGCAGACAGAGCAGGGGTC ATTCGCTACGTAATTGGGGTGGGAAATGCCTTCAACAAACCACAGTCCCGCAGAGAGCTCGACACCATCG CATCTAAGCCAGCTGGTGAACACGTGTTCCAAGTGGACAACTTTGAAGCCCTGAATACCATTCAGAACCA GCTTCAGGAAAAGATCTTTGCAATTGAGGGCACGCAGACAGGAAGTACCAGCTCCTTTGAGCATGAGATG TCTCAAGAAGGCTTCAGTGCTTCCATTACCTCTAATGGTCCCTTGCTGGGCTCTGTGGGGAGCTTTGACT GGGCAGGTGGAGCCTTCCTGTATACATCGAAGGATAAAGTCACCTTCATCAACACAACCAGAGTGGATTC AGACATGAATGATGCTTACCTGGGTTATGCTTCTGCAGTCATCTTGAGGAACCGTGTCCAAAGCTTGGTT TTAGGAGCACCTCGGTATCAGCATATTGGCTTGGTGGTGATGTTCAGGGAGAATTTCGGTACCTGGGAGC CCCACACTAGCATCAAGGGCAGCCAGATTGGCTCTTATTTTGGGGCCTCCCTTTGCTCTGTGGACATGGA CGCTGATGGCAATACCAACTTGATCCTCATTGGGGCCCCTCATTACTATGAGAAGACCCGAGGAGGCCAG GTGTCAGTGTGTCCCTTGCCTCGAGGGCAGAGGGCACGGTGGCAGTGTGAAGCTCTTCTCCACGGTGATC AGGGTCATCCCTGGGGTCGCTTTGGGGCAGCCCTGACAGTGCTGGGAGACGTGAATGGGGACAAACTGAC AGATGTGGCCATTGGGGCCCCAGGAGAGCAGGAGAATCAGGGCGCTGTCTACATTTTTTATGGAGCATCA ATAGCCAGCCTCAGTGCCTCCCACAGCCAGCGGATCATAGGCGCCCACTTCTCCCCTGGGCTCCAGTACT TCGGGCAGTCTCTGAGTGGGGGTAAGGATCTCACAATGGATGGCTTGATGGACCTGGCTGTTGGGGCGCA GGGGCATCTTCTGCTGCTCAGAGCCCAGCCGGTGCTGAGACTGGAGGCAACCATGGAATTCAGCCCCAAG AAAGTAGCAAGGAGTGTGTTTGCATGTCAAGAACAAGTATTAAAAAACAAGGATGCTGGGGAGGTCAGAG TCTGCCTCCGTGTCCGCAAGAACACCAAGGACCGTCTGCGCGAAGGAGATATCCAGAGCACTGTCACTTA TGACCTGGCTTTAGACCCTGGCCGCTCACGTATCCGTGCCTTCTTTGATGAGACAAAGAACAACACACGC AGGCGCACCCAGGTCTTTGGATTGATGCAGAAATGTGAAACACTGAAGCTAATTTTACCGGACTGCGTGG ACGACTCAGTGAGCCCCATCATCCTGCGCCTCAATTATACACTGGTTGGGGAGCCCTTGAGGTCCTTTGG GAACCTCCGACCAGTTCTGGCTATGGATGCTCAGAGGTTCTTCACAGCTATGTTTCCCTTTGAGAAAAAT TGCGGCAATGACAGCATCTGCCAAGACGATCTCAGCATCACCATGAGTGCCATGGGCTTGGACACTTTGG TGGTGGGAGGCCCCCAGGACTTTAACATGAGTGTGACTCTGAGAAATGACGGTGAGGATTCCTACGGGAC CCAGGTTACCGTCTACTACCCATCTGGCTTATCTTACCGGAAGGATTCAGCAAGCCAGAACCCGCTCACC AAGAAGCCTTGGTTTGTAAAGCCTGCTGAGTCCAGCTCTTCTTCTGAAGGGCATGGGGCTCTGAAGAGTA CCACCTGGAACATAAACCATCCCATCTTTCCTGCTAATTCTGAGGTCACATTTAATGTCACATTTGATGT GGACTCTCATGCCTCCTTTGGGAACAAACTGCTCCTCAAAGCCATTGTGGCCAGTGAGAACAACATGTCC AGGACCCACAAAACCAAGTTTCAGTTGGAGCTGCCTGTGAAGTACGCCATCTACATGATTGTCACCAGTG ATGAGAGTTCTATCAGATATCTCAACTTCACGGCTTCAGAGATGACCAGTAAGGTCATACAGCATCAGTA CCAGTTCAACAACCTGGGCCAGAGGAGCCTCCCTGTCAGTGTAGTCTTCTGGATCCCTGTTCAGATCAAC AATGTGACCGTATGGGATCATCCCCAGGTCATCTTCTCCCAGAACCTCTCAAGTGCCTGTCACACTGAGC AGAAATCCCCCCCTCACTCCAATTTCCGGGATCAGCTTGAAAGGACCCCAGTGCTGAACTGCTCTGTTGC AGTCTGTAAGAGAATTCAGTGTGACCTTCCATCCTTCAACACTCAGGAAATATTCAATGTCACCCTCAAG GGCAACCTATCATTTGACTGGTACATCAAGACTTCTCATGGTCACCTCCTGCTTGTGAGCAGCACTGAGA TCCTGTTTAATGACTCTGCGTTTGCCCTGCTTCCAGGGCAGGAGTCGTATGTGAGGTCTAAGACAGAGAC CAAAGTGGAGCCATATGAAGTTCACAATCCTGTACCACTCATTGTGGGCAGCTCCATTGGGGGCCTGGTG CTCTTGGCTCTCATCACTGCTGGCCTATACAAGCTTGGCTTTTTCAAGCGGCAGTACAAGGACATGATGA ATGAAGCTGCCCCCCAAGACGCCCCACCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227326 representing NM_001082960
Red=Cloning site Green=Tags(s) MTLKALLVTALALCHGFNLDTEHPMTFQENAKGFGQSVVQLGGTSVVVAAPQEAKAVNQTGALYQCDYST SRCHPIPLQVPPEAVNMSLGLSLAVSTVPQQLLACGPTVHQNCKENTYVNGLCYLFGSNLLRPPQQFPEA LRECPQQESDIVFLIDGSGSINNIDFQKMKEFVSTVMEQFKKSKTLFSLMQYSDEFRIHFTFNDFKRNPS PRSHVSPIKQLNGRTKTASGIRKVVRELFHKTNGARENAAKILVVITDGEKFGDPLDYKDVIPEADRAGV IRYVIGVGNAFNKPQSRRELDTIASKPAGEHVFQVDNFEALNTIQNQLQEKIFAIEGTQTGSTSSFEHEM SQEGFSASITSNGPLLGSVGSFDWAGGAFLYTSKDKVTFINTTRVDSDMNDAYLGYASAVILRNRVQSLV LGAPRYQHIGLVVMFRENFGTWEPHTSIKGSQIGSYFGASLCSVDMDADGNTNLILIGAPHYYEKTRGGQ VSVCPLPRGQRARWQCEALLHGDQGHPWGRFGAALTVLGDVNGDKLTDVAIGAPGEQENQGAVYIFYGAS IASLSASHSQRIIGAHFSPGLQYFGQSLSGGKDLTMDGLMDLAVGAQGHLLLLRAQPVLRLEATMEFSPK KVARSVFACQEQVLKNKDAGEVRVCLRVRKNTKDRLREGDIQSTVTYDLALDPGRSRIRAFFDETKNNTR RRTQVFGLMQKCETLKLILPDCVDDSVSPIILRLNYTLVGEPLRSFGNLRPVLAMDAQRFFTAMFPFEKN CGNDSICQDDLSITMSAMGLDTLVVGGPQDFNMSVTLRNDGEDSYGTQVTVYYPSGLSYRKDSASQNPLT KKPWFVKPAESSSSSEGHGALKSTTWNINHPIFPANSEVTFNVTFDVDSHASFGNKLLLKAIVASENNMS RTHKTKFQLELPVKYAIYMIVTSDESSIRYLNFTASEMTSKVIQHQYQFNNLGQRSLPVSVVFWIPVQIN NVTVWDHPQVIFSQNLSSACHTEQKSPPHSNFRDQLERTPVLNCSVAVCKRIQCDLPSFNTQEIFNVTLK GNLSFDWYIKTSHGHLLLVSSTEILFNDSAFALLPGQESYVRSKTETKVEPYEVHNPVPLIVGSSIGGLV LLALITAGLYKLGFFKRQYKDMMNEAAPQDAPPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001082960 |
ORF Size | 3465 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001082960.1, NP_001076429.1 |
RefSeq Size | 4682 bp |
RefSeq ORF | 3465 bp |
Locus ID | 16409 |
MW | 127.5 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223766 | Itgam (untagged) - Mouse integrin alpha M (Itgam), transcript variant 1, (10ug) |
CNY 8,040.00 |
|
MG227326 | Itgam (tGFP-tagged) - Mouse integrin alpha M (Itgam) transcript variant 1, (10ug) |
CNY 12,640.00 |
|
MR227326L3 | Lenti ORF clone of Itgam (Myc-DDK-tagged) - Mouse integrin alpha M (Itgam), transcript variant 1 |
CNY 13,400.00 |
|
MR227326L4 | Lenti ORF clone of Itgam (mGFP-tagged) - Mouse integrin alpha M (Itgam), transcript variant 1 |
CNY 13,400.00 |