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Psmd1 (NM_027357) Mouse Tagged ORF Clone
CAT＃: MR227375
- TrueORF®
Psmd1 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (Psmd1)
ORF Plasmid: tGFP
"NM_027357" in other vectors (4)
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CNY 6,976.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410026J11Rik; P112; S1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227375 representing NM_027357
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCACTTCAGCCGCTGGAATTATTTCTCTTCTGGATGAAGAAGAGCCACAGCTTAAGGAATTTGCAC TACACAAATTGAATGCAGTCGTGAATGACTTCTGGGCAGAGATTTCTGAGTCTGTAGATAAAATAGAAGT TTTATATGAAGATGAAGGTTTCCGAAGTCGGCAGTTTGCAGCCCTGGTGGCCTCGAAAGTGTTTTATCAC CTGGGGGCTTTTGAGGAGTCTCTGAATTATGCTCTTGGAGCAGGTGACCTCTTCAACGTCAATGATAACT CTGAATATGTGGAGACTATTATAGCAAAATGCATTGATCATTATACCAAACAGTGTGTGGAAAATGCAGA TTTGCCTGAAGGAGAAAAAAAGCCAATTGACCAGCGATTGGAAGGCATCGTGAATAAAATGTTCCAGCGA TGTCTCGATGATCACAAGTATAAGCAAGCGATTGGCATTGCTCTGGAGACCAGGAGACTGGATGTCTTTG AGAAGACTATACTGGAATCTAATGATGTCCCAGGAATGCTAGCTTACAGCCTCAAACTCTGCATGTCTTT AATGCAGAATAAACAGTTTCGGAATAAAGTATTGAGAGTCTTGGTTAAAATCTACATGAACTTGGAAAAG CCTGATTTCATCAATGTTTGTCAGTGCTTAATTTTCTTAGATGATCCTCAGGCTGTGAGTGATATTTTAG AAAAACTGGTAAAGGAGGACAACCTGCTGATGGCCTATCAGATTTGTTTTGATTTGTACGAAAGTGCTAG CCAGCAGTTCTTGTCATCTGTCATCCAGAATCTTCGAACTGTTGGTACCCCTATTGCTTCTGTGCCTGGA TCTACCAATACGGGGACTGTGCCAGGATCAGAGAAAGACAGTGACCCCATGGAGACGGAGGAGAAGACAG CCAGTGCAGTGGCTGGGAAGACACCAGATGCGAGTCCAGAGCCCAAGGACCAGACACTGAAAATGATTAA GATTTTAAGTGGTGAAATGGCTATTGAATTACACCTGCAGTTCCTAATACGAAATAATAACACAGACCTC ATGATTCTGAAAAACACAAAGGATGCAGTACGAAATTCTGTGTGTCATACAGCAACTGTAATAGCAAACT CATTCATGCACTGTGGTACTACCAGTGACCAGTTTCTTAGAGATAACTTGGAATGGTTGGCCAGAGCCAC CAACTGGGCCAAATTTACTGCAACGGCCAGCTTGGGTGTAATCCATAAGGGCCATGAGAAGGAAGCACTA CAGTTAATGGCAACATACCTTCCTAAGGACACTTCTCCAGGATCAGCCTATCAGGAAGGGGGCGGTCTCT ATGCATTGGGTCTCATTCATGCCAATCATGGTGGTGACATAATTGACTATCTCCTTAATCAGCTCAAGAA TGCCAGCAATGATATTGTTCGACATGGAGGCAGCCTGGGCCTTGGTTTGGCTGCAATGGGAACTGCACGT CAGGATGTGTATGATTTGCTGAAAACAAACCTGTATCAGGATGATGCTGTGACAGGGGAGGCAGCTGGCC TAGCCTTAGGTTTGGTTATGTTAGGTTCTAAAAATGCCCAGGCGATTGAGGATATGGTTGGCTATGCACA AGAGACTCAACACGAGAAGATTCTGCGCGGTCTGGCAGTTGGCATTGCGTTAGTGATGTACGGGAGGATG GAGGAGGCTGATGCTCTCATTGAGTCTCTCTGTCGTGACAAGGACCCAATTCTTAGAAGATCTGGAATGT ATACTGTAGCCATGGCTTATTGTGGCTCTGGTAACAACAAAGCCATTCGGCGCCTGCTACATGTTGCTGT AAGTGATGTCAATGATGATGTCAGAAGAGCAGCAGTGGAGTCACTTGGCTTCATTTTGTTCAGGACGCCT GAACAGTGTCCAAGTGTGGTTTCTTTGTTGTCAGAGAGTTATAACCCTCACGTGCGCTATGGAGCTGCAA TGGCCCTGGGAATCTGCTGTGCTGGTACAGGAAACAAGGAAGCAATTAATTTACTAGAGCCAATGACCAA CGACCCTGTGAACTACGTGAGGCAAGGGGCTCTGATAGCTTCAGCTCTCATCATGATCCAACAGACTGAG ATCACGTGTCCAAAGGTGAATCAGTTCCGGCAGCTGTATTCCAAAGTCATCAATGACAAACATGATGATG TCATGGCCAAATTTGGCGCTATTCTGGCCCAAGGCATACTGGATGCAGGTGGTCATAATGTCACAATCTC CTTACAGTCCAGGACTGGGCACACTCATATGCCGTCTGTGGTTGGCGTCCTTGTCTTCACCCAGTTCTGG TTCTGGTTTCCCCTTTCACACTTCCTGTCATTGGCTTATACTCCTACCTGTGTCATTGGCCTTAACAAGG ATTTAAAGATGCCGAAAGTTCAATATAAATCAAATTGTAAACCATCCACATTCGCATATCCTGCCCCTCT GGAAGTACCAAAAGAAAAAGAAAAGGAAAAGGTTTCCACCGCTGTGTTGTCTATTACTGCCAAAGCTAAA AAAAAAGAAAAAGAAAAAGAAAAAAAGGAGGAGGAGAAGATGGAAGTGGATGAAGCAGAGAAGAAGGAAG AGAAAGAGAAGAAAAAAGAACCTGAACCAAACTTCCAGCTGTTGGATAATCCAGCACGAGTTATGCCTGC CCAGCTGAAGGTCTTAAGTATGACAGAGACCTGCAGATACCAGCCTTTTAAACCACTCTCCATCGGGGGC ATCATAATTCTGAAAGACACCAGCGAAGACGTTGAAGAACTCGTGGAACCCGTGGCAGCACACGGCCCAA AGATTGAAGAGGAAGAGCAAGAGCCAGAGCCCCCAGAGCCGTTCGAGTATATCGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227375 representing NM_027357
Red=Cloning site Green=Tags(s) MITSAAGIISLLDEEEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYH LGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQR CLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEK PDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPG STNTGTVPGSEKDSDPMETEEKTASAVAGKTPDASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDL MILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEAL QLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTAR QDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRM EEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTP EQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTE ITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFW FWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAK KKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLSMTETCRYQPFKPLSIGG IIILKDTSEDVEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027357 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027357.2, NP_081633.1 |
| RefSeq Size | 3256 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 70247 |
| UniProt ID | Q3TXS7 |
| MW | 106.2 kDa |
| Gene Summary | In eukaryotic cells, most proteins in the cytosol and nucleus are degraded via the ubiquitin-proteasome pathway. The 26S proteasome is a self-compartmentalizing protease comprised of approximately 31 different subunits. It contains a barrel-shaped proteolytic core complex (the 20S proteasome), capped at one or both ends by 19S regulatory complexes, which recognize ubiquitinated proteins. Protein degradation by proteasomes is the source of most antigenic peptides presented on MHC class I molecules. This gene encodes a non-ATPase subunit of the 26S proteasome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222787 | Psmd1 (untagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (Psmd1), (10ug) |
CNY 12,160.00 |
|
| MG227375 | Psmd1 (tGFP-tagged) - Mouse proteasome (prosome macropain) 26S subunit non-ATPase 1 (Psmd1), (10ug) |
CNY 7,890.00 |
|
| MR227375L3 | Lenti ORF clone of Psmd1 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (Psmd1) |
CNY 9,030.00 |
|
| MR227375L4 | Lenti ORF clone of Psmd1 (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (Psmd1) |
CNY 9,030.00 |
