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Nos1 (NM_008712) Mouse Tagged ORF Clone
CAT＃: MR227419
- TrueORF®
Nos1 (Myc-DDK-tagged) - Mouse nitric oxide synthase 1, neuronal (Nos1)
ORF Plasmid: tGFP
"NM_008712" in other vectors (4)
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CNY 9,776.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227419 representing NM_008712
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGCACACGTTTGGGGTCCAGCAGATCCAACCCAACGTCATTTCTGTCCGTCTCTTCAAACGCA AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCCCCGGTGATCATCTCAGATCTGAT CCGAGGAGGTGCCGCGGAGCAGAGCGGCCTTATCCAAGCCGGCGACATCATTCTCGCCGTCAATGATCGG CCCCTGGTAGACCTCAGCTATGACAGCGCTCTGGAAGTTCTCAGGGGCATTGCCTCTGAGACCCATGTGG TCCTCATTCTGAGAGGCCCTGAGGGCTTTACTACACACCTGGAGACCACCTTCACAGGGGATGGAACCCC CAAGACCATCCGTGTGACCCAGCCCCTGGGGACACCCACCAAAGCTGTCGATCTGTCTCGCCAGCCATCA GCCAGCAAAGACCAGCCATTAGCAGTAGACAGGGTCCCAGGTCCCAGTAACGGACCTCAGCATGCCCAAG GCCGTGGACAGGGAGCCGGCTCAGTCTCCCAGGCTAATGGTGTGGCCATTGACCCCACAATGAAAAATAC CAAGGCCAACCTCCAGGACAGCGGAGAACAGGATGAACTGCTCAAAGAGATAGAACCTGTGTTGAGCATC CTCACCGGTGGGGGAAAAGCAGTCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC AGGTGGACAGAGACCTCGACGGCAAACTGCACAAAGCTCCGCCCCTGGGCGGGGAGAATGATCGAGTCTT CAATGATCTGTGGGGGAAGGGCAACGTCCCTGTGGTCCTCAACAACCCGTATTCAGAGAACGAACAGTCT CCCGCCTCGGGCAAACAGTCTCCTACCAAGAATGGCAGTCCTTCCAGGTGCCCTCGCTTCCTTAAGGTCA AGAACTGGGAGACAGATGTGGTCCTCACTGACACCCTGCACCTGAAGAGCACGCTGGAAACGGGGTGCAC AGAGCAGATCTGCATGGGCTCCATCATGCTGCCATCCCATCACATACGGAAGTCAGAAGATGTCCGCACC AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCCTCCATTAAGAGGTTTGGCT CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATCGAGAGCACCAGCACCTACCAGCTCAA GGACACGGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGCTGTGTGGGCAGGATCCAG TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCATGGGATGTTCAACTACATCTGTA ACCACGTCAAGTACGCCACCAACAAAGGGAACCTCAGGTCGGCCATCACTATATTCCCTCAAAGGACTGA TGGCAAGCATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTATGCCGGCTACAAGCAGCCAGATGGC TCTACCTTGGGCGATCCAGCTAATGTGGAGTTCACAGAGATCTGTATACAGCAGGGCTGGAAACCCCCAA GAGGCCGGTTCGATGTGCTGCCTCTCCTGCTCCAGGCCAATGGCAATGACCCTGAACTCTTTCAGATCCC CCCAGAGCTGGTGCTGGAAGTACCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATTGGGGGCCTGGAGTTCAGCGCCTGTCCCT TTAGTGGCTGGTACATGGGCACGGAGATTGGCGTTCGTGATTACTGTGACAACTCTCGATACAACATCCT GGAGGAAGTAGCCAAGAAAATGGATTTGGACATGAGGAAGACATCCTCCCTCTGGAAGGACCAAGCCCTG GTGGAGATTAACATTGCTGTCCTATACAGCTTCCAGAGTGACAAGGTGACCATTGTGGACCACCACTCTG CCACCGAGTCCTTCATCAAACACATGGAGAATGAGTACCGCTGCAGAGGGGGCTGTCCCGCCGACTGGGT GTGGATTGTGCCCCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTACCGGCTC ACCCCGTCCTTTGAGTACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAATGGGACCCCCA CGAAGCGGCGAGCCATTGGCTTCAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTGATGGGACA GGCCATGGCCAAGAGAGTCAAAGCGACCATTCTCTACGCCACCGAGACAGGCAAATCCCAAGCCTATGCC AAGACCCTGTGCGAGATCTTCAAGCATGCCTTCGATGCCAAGGCTATGTCCATGGAGGAATATGACATCG TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCAGAGAA TGGGGAGAAATTCGGCTGTGCTTTGATGGAGATGAGGCACCCCAACTCTGTGCAAGAGGAGAGGAAGAGC TACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGACTCCCGCAAGTCATCAGGCGATGGACCAGACC TCAGAGACAACTTTGAAAGTACTGGACCGCTGGCCAATGTGAGGTTCTCAGTCTTCGGCCTGGGCTCTCG GGCATACCCTCACTTCTGTGCCTTTGGGCACGCAGTGGACACTCTCCTGGAGGAGCTGGGCGGGGAGAGG ATTCTGAAGATGAGGGAGGGAGATGAGCTCTGCGGACAGGAAGAGGCTTTCAGGACCTGGGCTAAGAAAG TCTTCAAGGCAGCCTGTGATGTGTTCTGTGTGGGGGATGACGTCAACATTGAGAAGGCGAACAATTCCCT CATTAGCAATGACCGCAGCTGGAAGAGGAATAAATTCCGCCTCACTTATGTGGCAGAAGCTCCAGAGCTG ACCCAAGGTCTTTCCAATGTTCACAAAAAGCGAGTCTCAGCCGCCCGACTCCTCAGCCGCCAAAACCTGC AAAGTCCTAAATCCAGCCGATCGACCATCTTCGTGCGTCTCCACACCAACGGGAATCAGGAGTTGCAGTA CCAGCCAGGGGACCACCTGGGTGTCTTCCCTGGCAACCATGAGGACCTCGTGAATGCACTCATTGAACGG CTGGAGGACGCGCCGCCTGCCAACCACGTGGTGAAGGTGGAAATGCTGGAGGAAAGGAACACGGCTCTGG GTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCACCCTGCACCATCTTCCAGGCCTTCAAGTACTA CCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGCAGCAGTTCGCCTCCCTGGCCACCAATGAGAAA GAGAAGCAGCGGCTGCTGGTCCTCAGCAAGGGGCTTCAGGAATATGAGGAATGGAAATGGGGCAAGAACC CCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATCCAGATGCCGGCCACACTTCTCCTCACACAGCT GTCGCTGCTACAACCTCGCTACTATTCCATCAGCTCCTCTCCAGACATGTACCCCGACGAGGTGCACCTC ACCGTGGCCATCGTGTCCTACCATACCCGAGATGGAGAAGGACCGGTTCACCACGGGGTGTGCTCCTCCT GGCTCAACCGAATACAGGCTGACGATGTCGTCCCCTGCTTCGTGAGAGGTGCCCCTAGCTTCCACCTGCC TCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAGGCACCGGCATCGCTCCCTTCCGAAGTTTTTGG CAACAGCGACAATTTGACATCCAACACAAAGGAATGAATCCGTGCCCCATGGTTCTGGTCTTCGGGTGTC GACAATCCAAGATAGATCATATCTACAGAGAGGAGACCCTGCAGGCCAAGAACAAGGGCGTCTTCAGAGA GCTGTACACTGCCTACTCCCGGGAACCCGACAGGCCAAAGAAATATGTACAGGACGTGCTGCAGGAACAG CTGGCCGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGGAGGCCATATTTATGTCTGCGGGGACGTTACCA TGGCCGCTGATGTCCTCAAAGCCATCCAGCGCATAATGACCCAGCAGGGGAAACTCTCGGAGGAGGATGC TGGTGTGTTCATCAGCAGGCTGAGGGATGACAACCGGTACCACGAGGACATCTTTGGGGTCACCCTCAGA ACATATGAAGTGACCAACCGCCTTAGATCTGAGTCCATCGCCTTCATTGAAGAGAGCAAAAAAGACACAG ATGAGGTTTTCAGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227419 representing NM_008712
Red=Cloning site Green=Tags(s) MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDR PLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPS ASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSI LTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQS PASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRT KDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQ WSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDG STLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLK WYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQAL VEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRL TPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYA KTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKS YKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGER ILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPEL TQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIER LEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEK EKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHL TVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFW QQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQ LAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLR TYEVTNRLRSESIAFIEESKKDTDEVFSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008712 |
| ORF Size | 4287 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008712.3, NP_032738.1 |
| RefSeq Size | 4388 bp |
| RefSeq ORF | 4290 bp |
| Locus ID | 18125 |
| UniProt ID | Q9Z0J4 |
| MW | 160.9 kDa |
| Gene Summary | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224449 | Nos1 (untagged) - Mouse nitric oxide synthase 1, neuronal (Nos1), (10ug) |
CNY 18,430.00 |
|
| MG227419 | Nos1 (tGFP-tagged) - Mouse nitric oxide synthase 1 neuronal (Nos1), (10ug) |
CNY 11,376.00 |
|
| MR227419L3 | Lenti ORF clone of Nos1 (Myc-DDK-tagged) - Mouse nitric oxide synthase 1, neuronal (Nos1) |
CNY 14,440.00 |
|
| MR227419L4 | Lenti ORF clone of Nos1 (mGFP-tagged) - Mouse nitric oxide synthase 1, neuronal (Nos1) |
CNY 14,440.00 |
