Tbc1d7 (NM_001252640) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2610009C09Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228818 representing NM_001252640 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACGACTCTCAGAGGAACTTTCGATCAGTCTACTATGAGAAAGTCGGGTTTCGTGGTGTCGAAG AAAAGAAATCACTGGAAATCCTCCTGAAAGATGACCGTTTGGACATCGAGAAGCTTTGCACATTTAGCCA GAGGTTCCCTCTCCCATCCATGTATCGCGCGTTGGTATGGAAGGCGCTTCTAGGCATCTTACCTCCGCAC CATGACACTCATTCCCAGGTGATGGCCTACCGCAAAGACCAGTACCATGACATCCTCCATGCCCTGACAG TCGTCCGCTTCATCAGTGATGCCACGCCACAGGCTGAAGTGTATCTTCGCATGTATCAGCTTGAATCGGG GAAGCTACCTCGAAGTCCCTCTTTTCCTCTGGAGCCGGAGGATGAAGTCTTTCTTGCCATCGCCAAGGCC ATGGAAGAGATGGTGGAAGACAGTGTGGACTGTTACTGGATCAGCCGATGCTTCGTGAAGCAGTTAAATA ACAAGTACAGGGACGCTTTACCTCAGCTGCCCAAGGCTTTCGAGCAGTACTTGAATCTGGAAGACAGTAG GCTGCTGAGTCACCTGAAGACGTGTTCTGCAGTGTCCAAACTGCCTTACGACCTCTGGTTCCAAAGGTGC TTCGCGGGATGCCTCCCCGAGTCCAGTTTACAGAGGGTCTGGGATAAAGTCATTCCTCAGGACAGCTCAG ATGCCATCGTGAGCAAGGCCATCGACTTGTGGCACAAACACTGTGGGACCCCAGTGCATTCGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228818 representing NM_001252640 Red=Cloning site Green=Tags(s) MTDDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDIEKLCTFSQRFPLPSMYRALVWKALLGILPPH HDTHSQVMAYRKDQYHDILHALTVVRFISDATPQAEVYLRMYQLESGKLPRSPSFPLEPEDEVFLAIAKA MEEMVEDSVDCYWISRCFVKQLNNKYRDALPQLPKAFEQYLNLEDSRLLSHLKTCSAVSKLPYDLWFQRC FAGCLPESSLQRVWDKVIPQDSSDAIVSKAIDLWHKHCGTPVHSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252640
ORF Size	765 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252640.1, NP_001239569.1
RefSeq Size	1154 bp
RefSeq ORF	768 bp
Locus ID	67046
UniProt ID	Q9D0K0
MW	30.1 kDa
Gene Summary	Component of the TSC-TBC complex, that contains TBC1D7 in addition to the TSC1-TSC2 complex and consists of the functional complex possessing GTPase-activating protein (GAP) activity toward RHEB in response to alterations in specific cellular growth conditions. The small GTPase RHEB is a direct activator of the protein kinase activity of mTORC1 and the TSC-TBC complex acts as a negative regulator of mTORC1 signaling cascade by acting as a GAP for RHEB. Participates in the proper sensing of growth factors and glucose, but not amino acids, by mTORC1. It is unclear whether TBC1D7 acts as a GTPase-activating protein and additional studies are required to answer this question.[UniProtKB/Swiss-Prot Function]

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