Kcnab1 (NM_001289450) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Akr8a8; Kvbeta1.1; mKv(beta)1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229076 representing NM_001289450 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAATTGCCTACGAAAGTGGAGTTAATCTCTTCGACACAGCTGAGGTCTATGCTGCTGGGAAGGCTG AGGTGATTCTGGGAAGCATCATCAAGAAGAAAGGCTGGAGGAGGTCCAGCTTGGTCATCACAACCAAACT CTACTGGGGTGGAAAAGCTGAGACAGAAAGGGGACTGTCAAGAAAGCACATCATTGAAGGACTGAAAGGC TCCCTCCAGAGGCTGCAACTGGAATACGTGGATGTGGTCTTTGCAAATCGCCCAGACAGCAACACTCCCA TGGAAGAAATCGTTCGAGCCATGACGCACGTGATCAACCAAGGCATGGCCATGTACTGGGGCACCTCGAG GTGGAGCGCGATGGAGATCATGGAAGCCTACTCTGTCGCACGGCAGTTCAACATGATCCCGCCTGTCTGT GAGCAAGCTGAGTACCATCTTTTCCAGAGAGAGAAGGTGGAGGTCCAGCTGCCGGAGCTCTACCATAAAA TAGGAGTTGGTGCAATGACATGGTCTCCACTTGCTTGTGGAATTATTTCAGGAAAATATGGAAATGGGGT GCCAGAAAGTTCTAGAGCTTCACTGAAGTGCTACCAGTGGTTGAAGGAAAGAATCGTAAGTGAAGAAGGG AGAAAACAGCAAAACAAGCTGAAAGACCTCTCTCCAATCGCTGAGCGCCTGGGGTGCACGCTACCTCAGC TGGCTGTGGCGTGGTGCCTGAGAAATGAGGGTGTGAGTTCTGTGCTCCTGGGATCATCCACTCCGGAACA ACTCATTGAAAACCTTGGTGCCATTCAGGTCCTCCCTAAGATGACATCTCACGTGGTGAACGAGATTGAT AACATACTGCGCAACAAGCCCTACAGCAAAAAGGACTATAGATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229076 representing NM_001289450 Red=Cloning site Green=Tags(s) MTIAYESGVNLFDTAEVYAAGKAEVILGSIIKKKGWRRSSLVITTKLYWGGKAETERGLSRKHIIEGLKG SLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVINQGMAMYWGTSRWSAMEIMEAYSVARQFNMIPPVC EQAEYHLFQREKVEVQLPELYHKIGVGAMTWSPLACGIISGKYGNGVPESSRASLKCYQWLKERIVSEEG RKQQNKLKDLSPIAERLGCTLPQLAVAWCLRNEGVSSVLLGSSTPEQLIENLGAIQVLPKMTSHVVNEID NILRNKPYSKKDYRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289450
ORF Size	885 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289450.1, NP_001276379.1
RefSeq Size	2851 bp
RefSeq ORF	888 bp
Locus ID	16497
MW	33.6 kDa
Gene Summary	Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:10454353). Modulates action potentials via its effect on the pore-forming alpha subunits (PubMed:10454353). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (PubMed:8824288). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members (By similarity). Promotes the closure of KCNA1, KCNA2 and KCNA5 channels (By similarity). Accelerates KCNA4 channel closure (By similarity). Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4 (By similarity). Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (By similarity). Enhances KCNB1 and KCNB2 channel activity (PubMed:8824288). Binds NADPH; this is required for efficient down-regulation of potassium channel activity (By similarity). Has NADPH-dependent aldoketoreductase activity (By similarity). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.