Osbp2 (NM_001302631) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700095P05Rik; C630001G20Rik; Gm244; ORP-4; OSBPL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230255 representing NM_001302631 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCTGGAGAAGGCCAGAGGCAGCCAAGAAGACCCTGGGAGTTGGGGTGGAAGCCAGGAAGCTTCT TGACTTCCAAAGGAGAGAACAGTGAGGAAGATGAAGATACTGAGTACTTTGACGCCATGGAAGACTCTAC ATCTTTCATCACCGTAGTCACTGAGGCCAAGGAAGACAGAAAGCCTGAGAGTGGACCTGGGACCACCACT GTGGACTGGACCTCAGCAGATAATGTATTAGATGGTGCCTCATTCATGCCCAAGAATTCATGCAAAGTTA AGAGGCGAGTCCGCATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATCATGAAGAATTGTAT TGGCCGAGAGCTTTCCCGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGACTT ACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTCGGTGGAGCAGATGT GCCTGGTAGCCGCCTTTTCTGTGTCCTCCTACTCCACCACGGTGCACCGCATCGCCAAGCCCTTCAACCC TATGCTCGGGGAGACCTTCGAGCTGGACCGTATGGAGGACATGGGCCTGCGTTCCCTCTGTGAGCAGGTG AGCCACCACCCCCCGTCTGCTGCCCACCACGTGTTCTCTAAGCATGGCTGGAGCCTCTGGCAAGAAATCA CCATCGCCAGCAAGTTCCGAGGGAAATACATCTCTATCATGCCACTCGGTGCCATCCACCTAGAATTCCA GGCCAGTGGCAATCACTACGTGTGGAGGAAGAGCACCTCCACCGTGCACAACATCATCGTGGGCAAGCTC TGGATTGACCAGTCAGGGGACATTGAGATTGTGAACCACAAGACCAAGGACCGGTGCCAGCTGAAGTTCG TACCCTACAGCTACTTCTCCAAAGAGGCAGCCCGAAAGGTGACTGGAGTGGTGAGTGACAGCCAGGGCAA GGCCCACTACGTGCTGTCAGGTTCGTGGGATGACCAGATGGAATGTTCTAAGATTGTGCACAGCAGCCCC AGCTCTGATGGGAGACAGAAAACCGTGTACCAGACACTGCCCGCCAAACTGCTCTGGAGGAAATACCCAC TGCCGGAGAATGCGGAGAACATGTACTATTTCTCCGAGCTAGCCCTGACCCTCAACGAGCAGGAGGACGG CGTGGCGCCCACCGACAGTCGCCTGCGGCCAGACCAGCGGCTTATGGAGAGGGGACGCTGGGACGAGGCC AACACCGAGAAGCAACGGCTGGAGGAGAAGCAGCGCCTGTCCAGGCGACGGCGGCTGGAGTCATGCACGG CAGGCTGCGGTGGGGAGGAAGCAGAGAAGGAGTCAGATGGCTATGTGCCGCTCTGGTTCGAGAAGAGGCT GGACCCGCTGACTGGGGAGATGGCCTGCATGTACAAGGGCGGCTACTGGGAGGCCAAGGAGAAGAAGGAC TGGCACATGTGCCCCAACATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230255 representing NM_001302631 Red=Cloning site Green=Tags(s) MRPGEGQRQPRRPWELGWKPGSFLTSKGENSEEDEDTEYFDAMEDSTSFITVVTEAKEDRKPESGPGTTT VDWTSADNVLDGASFMPKNSCKVKRRVRIPDKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRL TEDLEYHHLLDKAVNCTSSVEQMCLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRMEDMGLRSLCEQV SHHPPSAAHHVFSKHGWSLWQEITIASKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTSTVHNIIVGKL WIDQSGDIEIVNHKTKDRCQLKFVPYSYFSKEAARKVTGVVSDSQGKAHYVLSGSWDDQMECSKIVHSSP SSDGRQKTVYQTLPAKLLWRKYPLPENAENMYYFSELALTLNEQEDGVAPTDSRLRPDQRLMERGRWDEA NTEKQRLEEKQRLSRRRRLESCTAGCGGEEAEKESDGYVPLWFEKRLDPLTGEMACMYKGGYWEAKEKKD WHMCPNIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302631
ORF Size	1494 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302631.2
RefSeq Size	2916 bp
RefSeq ORF	1497 bp
Locus ID	74309
MW	57.6 kDa
Gene Summary	The protein encoded by this gene belongs to the oxysterol-binding protein-related family of proteins, which are defined by a C-terminal sterol domain with a highly conserved EQVSHHPP motif. Oxysterols are oxygenated derivatives of cholesterol that are involved in mechanisms that include apoptosis, cholesterol homeostasis, lipid trafficking and cell differentiation. This protein is selectively expressed at high levels in the brain and testis. Within the testis, the mRNA is localized to postmeiotic germ cells, including spermatids and spermatozoa, but is not detectable in somatic cells. Mice homozygous mutant for a targeted deletion in this gene do not exhibit overt developmental phenotypes but are male sterile. Females display normal fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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