Shmt2 (NM_001252316) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2700043D08Rik; AA408223; AA986903
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230267 representing NM_001252316 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATCCTTCTCTTTGCTTCGGACCACTCGGAGATGTGGTCAGCTGGTCTGCATGGCTGCCCGGGCCC AGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAGCTGGAGGTTGGACGGGCCAGGAGAGTTTATC AGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAGACAGTGTCGCGGCCTGGAGCTC ATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGGTCCTGTCTCAACAACAAGTACT CGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGGACGAGATCGAGCTGCTCTGCCA GCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGTCAATGTGCAGCCATACTCAGGG TCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGATCGAATCATGGGGTTGGACCTGC CCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGATCTCCGCCACATCCATTTTCTT CGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTACGACCAGCTGGCGCTGACCGCT CGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCCCGCCTCATTGACTATGCACGCA TGAGAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGGCCCACATCAGTGGCCTGGTGGC TGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCACCACCACTCACAAGACACTGCGA GGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTAGACCCCAAGACTGGCAAAGAGA TCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCCTACAGGGTGGCCCCCACAACCA CGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTATGTTCCGCGAGTACTCCTTACAA GTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGATACTCGCTGGTGTCTGGTGGCA CTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATGGAGCCCGAGCCGAACGCGTGTT GGAACTCGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCGGAGCGCCATTACTCCGGGGGGC TTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGACGACTTCCGTAGAGTCGTCGATT TTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTGCCAAGCTCCAGGATTTCAAATC CTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCGGCAACAGGTGGAACAGTTTGCC AGGGGCTTCCCCATGCCTGGATTTGATGAACGT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230267 representing NM_001252316 Red=Cloning site Green=Tags(s) MVSFSLLRTTRRCGQLVCMAARAQHSKVAQTQAGEAAGGWTGQESLSDSDPEMWELLQREKDRQCRGLEL IASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSG SPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLALTA RLFRPRLIIAGTSAYARLIDYARMREVCDEVRAHLLADMAHISGLVAAKVIPSPFKYADVVTTTTHKTLR GARSGLIFYRKGVRTVDPKTGKEIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQ VLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGG LRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVEQFA RGFPMPGFDER SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252316
ORF Size	1503 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252316.1, NP_001239245.1
RefSeq Size	2296 bp
RefSeq ORF	1506 bp
Locus ID	108037
UniProt ID	Q9CZN7
MW	55.9 kDa
Gene Summary	Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (By similarity). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (By similarity). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (By similarity). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640). Associates with mitochondrial DNA (By similarity). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.