Arntl2 (NM_001289679) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4632430A05Rik; bHLHe6; BMAL2; CLIF; MOP9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230545 representing NM_001289679 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTCCAAGGAAACGCAGAGGCAGAGATTCCCAGCCACTCCAGTCAGAATTCATGACAGACACAA CAGTGGAAAGTCTTCCCCAGAATCCCTTTGCCTCTCTTCTTTCAACAAGAACAGGAGTATCAGCGCCCAG TGGCATCAGGGAAGCTCACAGCCAGATGGAAAAGCGTCGGAGAGACAAGATGAACCATCTGATTCAGAAA CTGTCATCTATGATCCCTCCACACATCCCCACGGCCCACAAACTGGACAAGCTCAGCGTCTTGAGGAGGG CGGTGCAGTACTTGAGGTCTCTGAGAGGCATGACAGAGCTTTACTTAGGAGAAAACTCTAAACCTTCATT TATTCAGGATAAGGAACTCAGTCACTTAATCCTCAAGGCAGCAGAAGGCTTCCTGTTTGTGGTTGGATGC GAAAGAGGGAGAATTTTTTACGTTTCTAAGTCTGTCTCCAAAACACTGCGTTATGATCAGGCTAGCTTGA TAGGACAGAATTTGTTTGACTTCTTACACCCAAAAGACGTCGCCAAAGTAAAGGAACAACTTTCTTGTGA TGGTTCACCAAGAGAGAAACCTATAGACACCAAAACCTCTCAGGTTTACAGTCACCCCTACACTGGGCGA CCACGCATGCATTCTGGCTCCAGACGATCTTTCTTCTTTAGAATGAAGAGCTGTACCGTCCCTGTCAAAG AAGAGCAGCCATGCTCGTCCTGCTCAAAGAAGAAAGACCATAGAAAATTCCACACCGTCCATTGCACTGG ATACTTGAGAAGCTGGCCTCTGAATGTTGTTGGCATGGAGAAAGAGTCGGGTGGTGGGAAGGACAGCGGT CCTCTTACCTGCCTTGTGGCTATGGGACGGTTGCATCCATACATTGTCCCTCAAAAGAGTGGCAAGATCA ACGTGAGACCGGCTGAGTTCATAACTCGCTTCGCAATGAACGGGAAATTCGTCTATGTTGACCAAAGGGC AACGGCAATTTTAGGATACCTGCCTCAGGAACTTTTGGGAACTTCATGTTATGAATATTTTCATCAGGAT GACCACAGTAGTTTGACTGACAAGCACAAAGCAGACTCATACAAATTCAGAGTGAAGGATGGTGCCTTCG TGACTCTGAAGAGTGAGTGGTTCAGCTTCACAAACCCTTGGACCAAAGAGCTGGAGTACATTGTGTCTGT CAACACATTGGTTTTGGGGCGCAGTGAGACCAGGCTGTCTTTGCTTCATTGCGGCGGCAGCAGCCAGTCC TCCGAAGACTCATTTAGACAATCCTGCATCAATGTGCCCGGTGTATCCACGGGGACCGTCCTTGGTGCTG GGAGTATTGGAACAGATATTGCAAATGAGGTTCTGAGTTTACAGAGATTACACTCTTCATCCCCAGAAGA TGCAAGCCCTTCAGAAGAAGTGAGAGATGACTGCAGTGTAAATGGTGGGAATGCCTATGGGCCTGCATCC ACTAGGGAGCCTTTTGCAGTGAGCCCTTCTGAAACAGAGGTCCTGGAGGCTGCCAGGCAACACCAGAGCA CTGAACCCGCCCACCCTCACGGACCACTTCCCGGTGACAGTGCCCAGCTGGGTTTTGATGTCCTGTGTGA CAGTGACAGCATAGACATGGCTGCATTCATGAATTACCTCGAAGCAGAGGGGGGCCTGGGTGACCCTGGG GACTTCAGTGACATCCAGTGGGCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230545 representing NM_001289679 Red=Cloning site Green=Tags(s) MEFPRKRRGRDSQPLQSEFMTDTTVESLPQNPFASLLSTRTGVSAPSGIREAHSQMEKRRRDKMNHLIQK LSSMIPPHIPTAHKLDKLSVLRRAVQYLRSLRGMTELYLGENSKPSFIQDKELSHLILKAAEGFLFVVGC ERGRIFYVSKSVSKTLRYDQASLIGQNLFDFLHPKDVAKVKEQLSCDGSPREKPIDTKTSQVYSHPYTGR PRMHSGSRRSFFFRMKSCTVPVKEEQPCSSCSKKKDHRKFHTVHCTGYLRSWPLNVVGMEKESGGGKDSG PLTCLVAMGRLHPYIVPQKSGKINVRPAEFITRFAMNGKFVYVDQRATAILGYLPQELLGTSCYEYFHQD DHSSLTDKHKADSYKFRVKDGAFVTLKSEWFSFTNPWTKELEYIVSVNTLVLGRSETRLSLLHCGGSSQS SEDSFRQSCINVPGVSTGTVLGAGSIGTDIANEVLSLQRLHSSSPEDASPSEEVRDDCSVNGGNAYGPAS TREPFAVSPSETEVLEAARQHQSTEPAHPHGPLPGDSAQLGFDVLCDSDSIDMAAFMNYLEAEGGLGDPG DFSDIQWAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289679
ORF Size	1707 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289679.1, NP_001276608.1
RefSeq Size	2593 bp
RefSeq ORF	1710 bp
Locus ID	272322
UniProt ID	Q2VPD4
MW	63.7 kDa
Gene Summary	This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (Per, Arnt, Sim) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian Clock protein, the structurally related Mop4, and hypoxia-inducible factors, such as Hif1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]

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