Flcn (NM_001271356) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU014660; B430214A04Rik; Bhd; FLCL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230570 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGCCATAGTCGCCCTCTGCCACTTCTGCGAGCTCCATGGCCCCCGCACGCTCTTCTGCACGGAAG TTCTACACGCTCCCCTGCCCCAGGGGGCCGGAAGTGGGGACAGTCCTGACCAGGTTGAGCAGGCTGAGGA GGAGGAGGGTGGCATTCAGATGAGCAGCCGGGTCCGTGCCCACAGCCCAGCCGAGGGTGCCAGCAGTGAG TCCAGCAGCCCGGGGCCCAAGAAGTCGGACATGTGTGAGGGCTGCCGGTCACTTGCCGTAGGGCACCCAG GCTATATCAGTCATGATAAAGAGACCTCTATTAAGTACGTCAGTCACCAGCACCCCAACCACCCGCAGCT CTTCAGCATCGTCCGCCAGGCCTGTGTCCGGAGCCTGAGCTGTGAGGTATGCCCTGGTCGTGAAGGCCCC ATCTTCTTTGGTGATGAGCAGCACGGCTTTGTGTTCAGCCACACCTTCTTCATCAAAGACAGCCTGGCCA GAGGCTTCCAGCGCTGGTACAGCATCATCGCCATCATGATGGATCGGATCTACCTCATCAACTCCTGGCC CTTCCTGCTGGGGAGGATCCGCGGCATCATCAGTGAGCTCCAGGCCAAGGCCTTCAAGGTGTTTGAGGCA GAGCAGTTTGGATGTCCACAGCGTGCCCAGAGGATGAACACTGCCTTCACGCCCTTCCTGCACCAGAGGA ACGGCAACGCTGCCCGCTCTCTGACCTCCTTGACCAGTGATGACAACTTGTGGGCGTGTCTGCACACTTC CTTTGCCTGGCTCCTGAAGGCATGCGGTAGCAGGCTGACAGAAAAGCTCTTAGAGGGCGCTCCCACAGAG GACACCCTGGTCCAGATGGAGAAGCTTGCTGACTTGGAGGAAGAATCAGAAAGTTGGGACAATTCTGAGG CTGAGGAGGAGGAGAAAGCTCCTGTTACACCAGAGGGTGCTGAAGGGCGAGAGCTGACCAGTTGCCCAAC AGAGTCATCCTTTCTCTCAGCCTGTGGGAGCTGGCAGCCCCCAAAGCTTACCGGCTTCAAGTCTCTTCGA CACATGAGACAGGTCTTGGGTGCTCCATCCTTCCGTATGTTGGCTTGGCATGTCCTCATGGGGAATCAGG TGATCTGGAAAAGCAGAGATGTGAACCTGGTCCATTCAGCGTTTGAAGTCCTCCGGACCATGCTGCCTGT GGGCTGTGTCCGCATCATCCCTTACAGCAGCCAGTATGAGGAGGCCTATCGCTGCAACTTCCTGGGGCTC AGCCCTCCCGTGCCTATCCCTGCCCATGTTCTGGCCTCAGAGTTCGTAGTTGTCGTGGAGGTCCACACGG CCACTCGCTCAAACCTCCACCCTGCTGGGTGCGAGGATGACCAGTCCCTCAGCAAGTATGAGTTTGTGGT GACCAGTGGTAGCCCTGTGGCTGCAGACAGAGTTGGGCCCACTATCCTGAATAAGATTGAAGCAGCTCTG ACCAACCAGAACCTGTCTGTGGATGTGGTGGACCAATGTCTCATCTGCCTCAAGGAGGAATGGATGAACA AAGTGAAAGTCCTGTTTAAATTCACCAAGGTAGACAGTCGCCCCAAGGAGGACACACAGAAGCTCCTAAG CGTCCTAGGCGCATCAGAGGAGGACAACGTCAAACTGCTGAAGTTCTGGATGACGGGACTGAGCAAAACC TACAAGTCCCATCTCATGTCCACCGTCCGAAGCCCCACAGCTACAGAGTCACGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230570 protein sequence Red=Cloning site Green=Tags(s) MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGAGSGDSPDQVEQAEEEEGGIQMSSRVRAHSPAEGASSE SSSPGPKKSDMCEGCRSLAVGHPGYISHDKETSIKYVSHQHPNHPQLFSIVRQACVRSLSCEVCPGREGP IFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIIAIMMDRIYLINSWPFLLGRIRGIISELQAKAFKVFEA EQFGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTE DTLVQMEKLADLEEESESWDNSEAEEEEKAPVTPEGAEGRELTSCPTESSFLSACGSWQPPKLTGFKSLR HMRQVLGAPSFRMLAWHVLMGNQVIWKSRDVNLVHSAFEVLRTMLPVGCVRIIPYSSQYEEAYRCNFLGL SPPVPIPAHVLASEFVVVVEVHTATRSNLHPAGCEDDQSLSKYEFVVTSGSPVAADRVGPTILNKIEAAL TNQNLSVDVVDQCLICLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSVLGASEEDNVKLLKFWMTGLSKT YKSHLMSTVRSPTATESRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271356
ORF Size	1740 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271356.1, NP_001258285.1
RefSeq Size	3420 bp
RefSeq ORF	1740 bp
Locus ID	216805
UniProt ID	Q8QZS3
MW	64.3 kDa
Gene Summary	May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.[UniProtKB/Swiss-Prot Function]

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