Tnip1 (NM_001271455) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ABIN; ABIN-1; ABIN1; AU018810; Naf1; Nef; VAN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230619 representing NM_001271455 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCGTCCAGACTCCGGCAGAAGGCAGAGGAGCTGGTCAAGGACAGCGAGCTGTCACCACCGACAT CTGCCCCCTCCTTGGTCTCCTTTGATGACCTGGCTGAGCTCACAGGACAGGATACAAAGGTCCAGGTACA TCCTGCTACCAGCACTGCCGCCACCACCACCGCCACCGCCACCACGGGAAACTCCATGGAGAAGCCCGAG CCAGCCTCCAAATCTCCGTCCAATGGCGCCTCCTCGGACTTTGAAGTGGTCCCTACTGAGGAGCAGAATT CACCCGAAACTGGCAGCCACCCTACGAACATGATGGACCTGGGGCCCCCACCCCCAGAGGACAGCAACCT GAAGCTCCACCTGCAGCGCCTGGAGACCACCCTTAGCGTGTGTGCAGAGGAGCCAGACCACAGCCAGCTC TTCACCCACCTGGGCCGCATGGCCCTCGAGTTCAACAGGTTGGCCTCCAAAGTGCATAAAAATGAGCAGC GCACCTCCATCCTGCAGACCTTATGTGAGCAGCTGCGCCAGGAGAATGAAGCCCTGAAGGCCAAGCTGGA CAAGGGCCTGGAACAGCGGGATCTGGCTGCTGAGAGGCTGCGGGAGGAAAACACGGAGCTCAAGAAACTG TTGATGAACAGCAGCTGCAAAGAGGGACTCTGTGGGCAGCCCAGCTCCCCAAAGCCAGAGGGTGCTGGCA AGAAGGGCGTGGCTGGACAGCAGCAGGCCAGTGTGATGGCGAGTAAAGTCCCTGAAGCGGGGGCCTTTGG AGCAGCTGAGAAGAAGGTGAAGTTGCTAGAACAGCAACGCATGGAGCTGCTGGAAGTGAACAAGCAGTGG GACCAGCATTTCCGGTCCATGAAGCAGCAGTATGAGCAGAAGATCACAGAGCTTCGCCAGAAGCTGGTGG ACCTGCAGAAACAGGTAACTGAGCTGGAGGCCGAACGGGAGCAGAAGCAGCGTGACTTTGACCGGAAACT CCTCCTGGCCAAATCGAAGATAGAGATGGAAGAGACCGACAAGGAGCAGCTGACAGCAGAGGCCAAGGAA CTGCGCCAGAAGGTCAGGTACCTACAGGATCAGCTGAGCCCGCTCACAAGGCAACGAGAATACCAGGAGA AGGAGATCCAGCGGCTCAATAAGGCCCTGGAGGAGGCCCTCAGCATCCAGGCCTCTCCATCATCTCCGCC TGCAGCTTTTGGGAGTCCAGAAGGCGTTGGGGGCCATCTGAGGAAGCAGGAACTAGTGACACAGAATGAG TTGCTGAAACAGCAGGTAAAGATCTTTGAAGAGGACTTCCAGAGGGAACGGAGTGACCGTGAACGCATGA ATGAAGAGAAGGAGGAGCTGAAGAAGCAAGTAGAGAAGCTGCAGGCCCAGGTCACCCTGACTAATGCCCA GCTCAAAACTCTCAAAGAGGAGGAGAAGGCCAAGGAAGCCCTCAAACAGCAGAAGAGGAAAGCAAAGGCT TCGGGAGAGCGCTACCACATGGAACCCCACCCTGAGCACGTCTGCGGCGCCTATCCCTATGCCTACCCAC CCATGCCAGCCATGGTACCTCACCATGCCTACAAGGACTGGTCCCAGATCCGATACCCTCCACCCCCTGT GCCCATGGAGCACCCGCCCCCACACCCCAACTCTCGCCTCTTCCATCTGCCGGAGTACACCTGGCGTCCA CCCTGTGCAGGGATTCGGAATCAGAGCTCTCAAGTGATGGACCCGCCCCCAGACAGGCCTGCAGAGCCAG AGTCTGCAGACAATGACTGTGATGGGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230619 representing NM_001271455 Red=Cloning site Green=Tags(s) MEASRLRQKAEELVKDSELSPPTSAPSLVSFDDLAELTGQDTKVQVHPATSTAATTTATATTGNSMEKPE PASKSPSNGASSDFEVVPTEEQNSPETGSHPTNMMDLGPPPPEDSNLKLHLQRLETTLSVCAEEPDHSQL FTHLGRMALEFNRLASKVHKNEQRTSILQTLCEQLRQENEALKAKLDKGLEQRDLAAERLREENTELKKL LMNSSCKEGLCGQPSSPKPEGAGKKGVAGQQQASVMASKVPEAGAFGAAEKKVKLLEQQRMELLEVNKQW DQHFRSMKQQYEQKITELRQKLVDLQKQVTELEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKE LRQKVRYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQASPSSPPAAFGSPEGVGGHLRKQELVTQNE LLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKA SGERYHMEPHPEHVCGAYPYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRP PCAGIRNQSSQVMDPPPDRPAEPESADNDCDGPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271455
ORF Size	1782 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271455.1, NP_001258384.1
RefSeq Size	2719 bp
RefSeq ORF	1785 bp
Locus ID	57783
UniProt ID	Q9WUU8
MW	67.6 kDa
Gene Summary	Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases.[UniProtKB/Swiss-Prot Function]

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