Ano10 (NM_001271873) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI604832; Tmem16k
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230642 representing NM_001271873 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGTGACTTTATCAACGCTGGATACTTGTGAGAGCTCCTTCACACCTCTAGTGGTCATAGAACTCG CCCAGGATGTCAAAGATGAAACCAAAGAATGGCTCAAAAACAGAATCATTGCCAAGAAGAAAGATGGAGG TGCCCAGCTATTGTTTAGACCACTGCTAAATAAGTATGAGAAAGAAACCCTAGAAAACCAGAATTTATAC CTTGTTGGTGCCTCCAATGTCCGACTGTTACTGGGGGCAGAAGCAGTCGGTCTGGTGAAGGAATGTACGG ACGCTGCCATGCGGGCCTTCACCTATGGAACACGCCACAACTTTAAAGGTTTCCATGATAACAATAATGA CTTCCTCACCATGGCTGAGTGTCAGTTCATTATCAAACACGAACTTGAGAATCTTAGAGCTAGAGACGAG AAGATGATCCCTGGCTACCCACAGGCCAAACTGTACCCTGGGAAGTCGCTGATGAGGAGACTGCTCACGT CTGGCATCGTGACCCAAGTGTTCCCGCTGCATGACACTGAAGCCCTGAAGAAGCTGGAGGACACGTGGTA CACTCGGTTTGCTCTCAAGTACCAGCCCATAGTGATCCTGGAGGTGTGGAAGCGTGGCTGTGCCAACATG ACCTATCGCTGGGGGACTCTGGTCATGAAGAGGCAGTTTGAGGAGCCCCGGCCAGGGTTTCATGGAGTCC TTGGCATCAATTCAGTCACGGGCCGGGAGGAGCCCCTCTACTCGAGCTACAAGAGACAACTGCGCATCTA CCTAGTCTCTCTGCCATTTGTGTGCCTCTGCCTTTATTTCTCCCTTTATGTCATGATGATCTACTTTGAC ATGGAGGACTGGGCCTTGAGTCTCCACGAGGACAGTGGGTCTGAGTGGACCAGCCTTCTGCTGTATGTAC CCAGCATTGTTTATGCCGTTGTGATTGAGATCATGAACCGCCTCTATCGATATGCTGCCGAGTTCCTAAC ATCTTGGGAGAATCACAGATTGGAATCTGCCTACCAGAATCATCTCGTTCTGAAAGTGTTGGTGTTCAAC TTCCTGAATTGCTTCGCCTCACTCTTCTACATTGCCTTTGTCCTGAAGGACATGAAGCTTTTGCGCCAGA GCTTGGCCACACTCCTGATCACCTCCCAGATTCTGAACCAAGTCGTAGAATCTCTTCTTCCTTACTGGCT GCAGCGGAAGTACTGTGCGAGGGTAAAGCGGAAGGTTCAGGCATTAAAGTCTGAGGTCGACACAACCTTG TATGAGCAAGTCCTCCTGGAGAAGGAAATGGGAACGTACCTGGGAACCTTTGATGATTACCTGGAGTTGT TCCTGCAGTTTGGCTATGTGAGCCTTTTCTCTTGTGTTTACCCGCTAGCAGCTGCCTTTGCTGTGTTAAA TAACTTCACTGAAGTCAACTCAGATGCCTTGAAAATGTGCAGGGTCTTCAAACGGCCATTTGCAGAACCT TCGGCCAGTATTGGCGTATGGCAGTTGGCTTTTGAAACGATGAGCGTTATATCCGTGGTCACTAACTGTG CTCTGATTGGAATGTCACCACAAGTGAATGCAGTCTTTCCAGAATCCAAAACAGACCTTGTTCTGATTGT GGTGGCCGTAGAGCATGCACTCTTGGCTCTGAAGTTCATACTCGCATTTGCCATCCCTGATAAACCACGG CACATCCAACAGAAACTGGCCAGATTGGAATTCGAATCTTTGGAGGCACTCAAGCAACAGCAAATGAAGC TGGTGGCAGAAAACCTGAAGGAGGAGTACCAGGAAGATGGGAAGGAAGCTACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230642 representing NM_001271873 Red=Cloning site Green=Tags(s) MRVTLSTLDTCESSFTPLVVIELAQDVKDETKEWLKNRIIAKKKDGGAQLLFRPLLNKYEKETLENQNLY LVGASNVRLLLGAEAVGLVKECTDAAMRAFTYGTRHNFKGFHDNNNDFLTMAECQFIIKHELENLRARDE KMIPGYPQAKLYPGKSLMRRLLTSGIVTQVFPLHDTEALKKLEDTWYTRFALKYQPIVILEVWKRGCANM TYRWGTLVMKRQFEEPRPGFHGVLGINSVTGREEPLYSSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFD MEDWALSLHEDSGSEWTSLLLYVPSIVYAVVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLVLKVLVFN FLNCFASLFYIAFVLKDMKLLRQSLATLLITSQILNQVVESLLPYWLQRKYCARVKRKVQALKSEVDTTL YEQVLLEKEMGTYLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFAEP SASIGVWQLAFETMSVISVVTNCALIGMSPQVNAVFPESKTDLVLIVVAVEHALLALKFILAFAIPDKPR HIQQKLARLEFESLEALKQQQMKLVAENLKEEYQEDGKEAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271873
ORF Size	1803 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271873.1, NP_001258802.1
RefSeq Size	2479 bp
RefSeq ORF	1806 bp
Locus ID	102566
UniProt ID	Q8BH79
MW	69.7 kDa
Gene Summary	This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to inhibit anion conductance. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012]

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