Ano10 (NM_001271873) Mouse Untagged Clone
CAT#: MC228431
Ano10 (untagged) - Mouse anoctamin 10 (Ano10), transcript variant 2
CNY 5,800.00
货期*
4周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI604832; Tmem16k |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC228431 representing NM_001271873
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGTGACTTTATCAACGCTGGATACTTGTGAGAGCTCCTTCACACCTCTAGTGGTCATAGAACTCG CCCAGGATGTCAAAGATGAAACCAAAGAATGGCTCAAAAACAGAATCATTGCCAAGAAGAAAGATGGAGG TGCCCAGCTATTGTTTAGACCACTGCTAAATAAGTATGAGAAAGAAACCCTAGAAAACCAGAATTTATAC CTTGTTGGTGCCTCCAATGTCCGACTGTTACTGGGGGCAGAAGCAGTCGGTCTGGTGAAGGAATGTACGG ACGCTGCCATGCGGGCCTTCACCTATGGAACACGCCACAACTTTAAAGGTTTCCATGATAACAATAATGA CTTCCTCACCATGGCTGAGTGTCAGTTCATTATCAAACACGAACTTGAGAATCTTAGAGCTAGAGACGAG AAGATGATCCCTGGCTACCCACAGGCCAAACTGTACCCTGGGAAGTCGCTGATGAGGAGACTGCTCACGT CTGGCATCGTGACCCAAGTGTTCCCGCTGCATGACACTGAAGCCCTGAAGAAGCTGGAGGACACGTGGTA CACTCGGTTTGCTCTCAAGTACCAGCCCATAGTGATCCTGGAGGTGTGGAAGCGTGGCTGTGCCAACATG ACCTATCGCTGGGGGACTCTGGTCATGAAGAGGCAGTTTGAGGAGCCCCGGCCAGGGTTTCATGGAGTCC TTGGCATCAATTCAGTCACGGGCCGGGAGGAGCCCCTCTACTCGAGCTACAAGAGACAACTGCGCATCTA CCTAGTCTCTCTGCCATTTGTGTGCCTCTGCCTTTATTTCTCCCTTTATGTCATGATGATCTACTTTGAC ATGGAGGACTGGGCCTTGAGTCTCCACGAGGACAGTGGGTCTGAGTGGACCAGCCTTCTGCTGTATGTAC CCAGCATTGTTTATGCCGTTGTGATTGAGATCATGAACCGCCTCTATCGATATGCTGCCGAGTTCCTAAC ATCTTGGGAGAATCACAGATTGGAATCTGCCTACCAGAATCATCTCGTTCTGAAAGTGTTGGTGTTCAAC TTCCTGAATTGCTTCGCCTCACTCTTCTACATTGCCTTTGTCCTGAAGGACATGAAGCTTTTGCGCCAGA GCTTGGCCACACTCCTGATCACCTCCCAGATTCTGAACCAAGTCGTAGAATCTCTTCTTCCTTACTGGCT GCAGCGGAAGTACTGTGCGAGGGTAAAGCGGAAGGTTCAGGCATTAAAGTCTGAGGTCGACACAACCTTG TATGAGCAAGTCCTCCTGGAGAAGGAAATGGGAACGTACCTGGGAACCTTTGATGATTACCTGGAGTTGT TCCTGCAGTTTGGCTATGTGAGCCTTTTCTCTTGTGTTTACCCGCTAGCAGCTGCCTTTGCTGTGTTAAA TAACTTCACTGAAGTCAACTCAGATGCCTTGAAAATGTGCAGGGTCTTCAAACGGCCATTTGCAGAACCT TCGGCCAGTATTGGCGTATGGCAGTTGGCTTTTGAAACGATGAGCGTTATATCCGTGGTCACTAACTGTG CTCTGATTGGAATGTCACCACAAGTGAATGCAGTCTTTCCAGAATCCAAAACAGACCTTGTTCTGATTGT GGTGGCCGTAGAGCATGCACTCTTGGCTCTGAAGTTCATACTCGCATTTGCCATCCCTGATAAACCACGG CACATCCAACAGAAACTGGCCAGATTGGAATTCGAATCTTTGGAGGCACTCAAGCAACAGCAAATGAAGC TGGTGGCAGAAAACCTGAAGGAGGAGTACCAGGAAGATGGGAAGGAAGCTACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001271873 |
Insert Size | 1806 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271873.1, NP_001258802.1 |
RefSeq Size | 2479 bp |
RefSeq ORF | 1806 bp |
Locus ID | 102566 |
UniProt ID | Q8BH79 |
Gene Summary | This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to inhibit anion conductance. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the coding region, compared to variant 1. The resulting isoform (2) spans the membrane six times (rather than eight as in isoform 1) and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR230642 | Ano10 (myc-DDK-tagged) - Mouse anoctamin 10 (Ano10), transcript variant 2 |
CNY 5,990.00 |
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