Rtn3 (NM_001271486) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RTN3-A1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230774 representing NM_001271486 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGTCGTCAGCGGCCACTCAGTCCCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAG CTCTCGGCGGCGGCGGCGGGAGCCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCGTG TGCGGATTCCTTTGTTTCTTCCTCTTCCTCTCAGCCTGTATCTATATTTTCGACCTCACAAGGTGACTGG ACAGAAGCATTTACAGAAGGAAAACCTGTAAGAGACTACCTCAGTTCCACAAAAGAAGCTGGTGGCAACG GTGTGCCAGGCAGTTCTCAGCTTCATTCTGAGCTGCCTGGCTCTATGCCTGAGAAATGGGTCTCAGGCTC TGGAGCAGCCACAGTGGAAGTAACTTTACCTAACCTGAGGGGTGCCTGGCCTAACTCTGTGATGGGGGAA GTCACAGAGGTTGATAGTTCTGGGGAATCTGATGACACAGTAATAGAGGACATCACAGAAAAACCTGACT CCCTTCCAAGTGCTGCTGCAAAAACAAGTGAAAGGGAAATCAAAGAGACTCCCAGTCGTGAAACTGTGAG GAGTGAAATGTGTGAAAACTCTGAGCAGCCGCAGGCCCAGCCAGAAACTCCTACTCAGAAGAGTCTGGAA GGTGAGGTGGCTTCACAAGTACCTAATACCCTGAATGAAGTCACACCTGAAAAGCTTGATATGACTAACA ACCCCAAAGTTTGCTCAGCAGCACCTCCAAGTGTTCTTAATGAGACAGGATTCTCACTAACTGTGCCAGC TTCTGCCAAGTTGGAATCTTTGCTTGGAAAATATGTTGAAGATACAGATGGTTCCTCCCCAGAGGACTTG ATGGCTGTCCTCACAGGAGCCGAGGAGAAGGGGATAGTGGATAAAGAGGAAGGTGATGTTTTGGAAGCAG TGTTAGAGAAGATAGCAGACTTTAAAAACACTTTGCCTGTGGAACTCTTGCATGAAAGTGAGCTAAGTGG TTCTGAAACCAAAAATATTAAAAGCAAATACAGTGAAGACAGCAGAGAAACAACTGGAGGTGCCCCTACG ATGTCTCCCGACTTAGAGCAGGAGCAGCTCACCATCAGAGCCATTAAAGAATTAGGAGAAAGGCAAGCTG AGAAGGTGCAGGATGAAGGAATATCTTCTGGAGGAAAACTCAAGCAAACCTTTGCTCCACAGTCTGGGCC ACAGAGTTCATCTGACATCCTAGAACACACAGATGTCAAAACTGGATCTGATCTTGGAATTCCCAAAAAT CCTACTATCATCAAAAACACTAGAATAGATTCTATTTCCAGCCTTACCAAGACTGAAATGGTTAACAAGA ATGTTCTAGCAAGACTTCTCAGTGATTTCCCAGTGCACGATCTGATTTTCTGGCGAGATGTGAAGAAGAC TGGGTTTGTCTTTGGCACCACACTGATCATGCTGCTCTCTCTGGCAGCCTTCAGTGTTATCAGTGTGGTC TCTTACCTCATCCTGGCTCTTCTCTCTGTCACCATCAGCTTCAGAGTCTATAAGTCTGTCATTCAAGCTG TGCAGAAGTCAGAAGAAGGACATCCATTCAAGGCCTACTTGGATGTGGACATTACCCTGTCTTCAGAAGC TTTCCACAACTACATGAATGCTGCGATGGTGCATGTCAACAAGGCCCTCAAACTCATTATTCGTCTCTTT CTGGTAGAAGACTTGGTTGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTG TTTTTAATGGAATTACCCTTCTGATTCTCGCCGAGCTGCTTGTCTTCAGCGTCCCAATTGTCTATGAGAA GTATAAGACACAGATTGACCACTATGTTGGGATTGCCCGGGATCAGACCAAGTCAATTGTTGAAAAGATC CAAGCAAAGCTTCCTGGAATCGCCAAAAAAAAGGCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230774 representing NM_001271486 Red=Cloning site Green=Tags(s) MAESSAATQSPSVSSSSSGAEPSALGGGGGSPGACPALGAKSCGSSCADSFVSSSSSQPVSIFSTSQGDW TEAFTEGKPVRDYLSSTKEAGGNGVPGSSQLHSELPGSMPEKWVSGSGAATVEVTLPNLRGAWPNSVMGE VTEVDSSGESDDTVIEDITEKPDSLPSAAAKTSEREIKETPSRETVRSEMCENSEQPQAQPETPTQKSLE GEVASQVPNTLNEVTPEKLDMTNNPKVCSAAPPSVLNETGFSLTVPASAKLESLLGKYVEDTDGSSPEDL MAVLTGAEEKGIVDKEEGDVLEAVLEKIADFKNTLPVELLHESELSGSETKNIKSKYSEDSRETTGGAPT MSPDLEQEQLTIRAIKELGERQAEKVQDEGISSGGKLKQTFAPQSGPQSSSDILEHTDVKTGSDLGIPKN PTIIKNTRIDSISSLTKTEMVNKNVLARLLSDFPVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVV SYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHVNKALKLIIRLF LVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKI QAKLPGIAKKKAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271486
ORF Size	1929 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271486.1, NP_001258415.1
RefSeq Size	4075 bp
RefSeq ORF	1932 bp
Locus ID	20168
UniProt ID	Q9ES97
MW	69.2 kDa
Gene Summary	May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress (By similarity). Induces the formation of endoplasmic reticulum tubules (PubMed:24262037).[UniProtKB/Swiss-Prot Function]

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