Brsk2 (NM_001276763) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4833424K13Rik; SAD-A; SADA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230853 representing NM_001276763 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCGACGGGGAAGGACGGCGGCGGGGCGCAGCACGCGCAGTATGTGGGGCCCTACCGGCTGGAGA AGACGCTGGGCAAGGGGCAGACAGGCTTGGTGAAGCTGGGAATCCACTGTGTCACTTGCCAGAAGGTCGC CATCAAAATCGTGAACCGTGAGAAGCTCAGTGAGTCGGTGCTGATGAAGGTGGAGCGAGAGATTGCCATC TTGAAGCTCATCGAGCATCCACATGTACTGAAGCTGCATGACGTCTATGAAAACAAAAAATATTTATACC TGGTGCTAGAACATGTGTCTGGGGGAGAGCTGTTCGACTACCTGGTGAAGAAGGGCCGGCTGACCCCCAA GGAGGCCCGCAAGTTCTTCCGGCAGATCATCTCTGCACTGGACTTCTGTCACAGCCACTCCATATGCCAT AGAGACTTGAAGCCAGAGAACCTGCTGCTAGATGAGAGGAACAACATCCGTATTGCAGACTTTGGCATGG CATCCCTGCAGGTGGGAGACAGCCTGCTGGAGACCAGCTGCGGATCTCCACACTATGCCTGTCCGGAAGT GATTCGGGGCGAGAAGTATGATGGCCGCAAGGCAGATGTGTGGAGCTGTGGTGTGATCCTGTTCGCCTTG CTGGTGGGGGCTCTGCCTTTTGATGATGACAACCTGCGGCAGTTGCTGGAGAAGGTCAAGCGTGGTGTGT TCCACATGCCACACTTTATCCCACCAGACTGCCAGAGTCTCCTGCGTGGCATGATTGAGGTGGATGCAGC TCGGCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGTGGCAAGAATGAGCCAGAGCCC GAACAGCCCATCCCACGCAAGGTGCAGATCCGCTCACTACCCAGCTTGGAAGACATTGACCCTGATGTGT TGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGATCTGCTATCTGAGGA GGAGAATCAGGAAAAGATGATTTATTTCCTCCTCCTGGATCGGAAAGAACGGTATCCAAGCCATGAGGAT GAGGACCTGCCCCCCAGGAATGAGATAGACCCTCCCCGGAAGCGTGTGGATTCCCCGATGCTGAACCGGC ATGGCAAGCGGCGACCTGAGCGCAAGTCCATGGAAGTGCTCAGTGTGACAGATGGTGGCTCCCCAGTGCC TGCACGGAGAGCCATTGAGATGGCCCAGCATGGCCAGAGTAAAGCAGTGTTCAGTAAAAGCCTGGATATC GCTGAAGCCCATCCCCAATTCAGCAAAGAAGACAGATCTCGATCCATCAGTGGTGCGTCCTCAGGCCTTT CTACAAGTCCACTCAGCAGTCCTCGGGTGACCCCTCACCCCTCACCAAGGGGTAGTCCCCTTCCTACCCC CAAAGGGACGCCTGTCCACACGCCAAAGGAGAGCCCAGCTGGCACACCCAACCCCACACCACCATCCAGC CCTAGTGTTGGAGGAGTGCCCTGGCGGACACGACTGAACTCCATCAAGAACAGCTTCCTGGGCTCACCTC GATTCCACCGCCGGAAACTCCAAGTTCCCACGCCAGAGGAGATGTCCAACCTGACCCCAGAATCCTCTCC AGAGCTGGCCAAGAAATCGTGGTTCGGGAACTTCATCAACCTGGAGAAGGAGGAGCAGATCTTTGTGGTG ATCAAGGACAAGCCCCTGAGCTCCATCAAGGCTGACATCGTTCATGCCTTCCTGTCGATCCCCAGCCTCA GCCACAGCGTTATTTCCCAGACAAGCTTCAGGGCTGAATACAAGGCCACAGGGGGCCCAGCAGTGTTCCA GAAGCCGGTCAAGTTCCAGGTGGACATCACCTACACTGAGGGCGGAGAGGCCCAGAAGGAGAATGGCATC TACTCAGTCACATTCACTTTACTCTCAGGCCCCAGTCGCCGCTTCAAGAGGGTGGTGGAGACCATCCAGG CCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGGAATTATCCCGAAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230853 representing NM_001276763 Red=Cloning site Green=Tags(s) MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAI LKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICH RDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFAL LVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEP EQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHED EDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQSKAVFSKSLDI AEAHPQFSKEDRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSS PSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVV IKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGI YSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSAQHLSGIIPKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276763
ORF Size	2025 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276763.1, NP_001263692.1
RefSeq Size	4113 bp
RefSeq ORF	2028 bp
Locus ID	75770
MW	76 kDa
Gene Summary	Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function]

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