Tcf12 (NM_001253862) Mouse Tagged ORF Clone
CAT#: MR230872
- TrueORF®
Tcf12 (myc-DDK-tagged) - Mouse transcription factor 12 (Tcf12), transcript variant 2
"NM_001253862" in other vectors (1)
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CNY 6,840.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | A130037E08Rik; ALF1; bHLHb20; HEB; HEBAlt; HTF-4; HTF4; ME1; REB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230872 representing NM_001253862
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCCCAGCAGCAGCGCATGGCCGCGATAGGGACCGACAAGGAGCTGAGCGACCTGCTGGACTTCA GTGCGATGTTTTCTCCACCTGTTAATAGTGGGAAAACGAGACCAACAACACTGGGAAGTAGTCAGTTCAG CGGGTCAGGTATGGATGAGCGTGGAGGAACAACATCTTGGGGAACAAGTGGTCAACCAAGCCCCTCCTAT GATTCATCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAACCC ACGAAGGCTTGTCCCCAACACCTTTCATGAACTCAAATCTGATAGGGAAAACATCAGAGAGAGGCTCATT TTCCCTGTACAGCAGAGACTCTGGACTCTCAGGCTGTCAGTCTAGTCTCCTGAGACAAGATCTAGGACTT GGGAGCCCGGCACAGCTGTCTTCTTCAGGAAAGCCTGGAACACCATACTACTCATTCTCTGCCACAAGTT CGAGAAGAAGACCGCTCCATGATTCTGTAGCCCTAGATCCTTTACAAGCGAAGAAAGTAAGAAAGGTGCC TCCTGGCCTACCTTCTTCTGTATATGCACCATCCCCAAATTCTGACGATTTCAACCGTGAATCTCCTAGT TACCCATCTCCCAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAGTT CTTCTGACCTTTGGAGTTCATCGAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCAC ATCCCACATGTCTCAGTCCAGTAGTTATGGCAGCCTTCATTCACATGACCGCTTGAGTTATCCTCCACAC TCAGTGTCACCAACAGACATAAACACAAGTCTTCCGCCAATGTCCAGCTTCCACCGTGGTAGTACCAGCA GCTCACCATATGTTGCCGCCTCACATACTCCTCCCATCAATGGATCAGATAGCATCCTAGGAACCAGAGG GAATGCTGCTGGAAGCTCACAGACGGGTGATGCACTTGGGAAGGCCTTGGCATCTATTTATTCCCCTGAC CACACAAGCAGTAGTTTTCCATCAAACCCATCAACACCAGTGGGATCTCCTTCACCTCTCACAGGTACCA GTCAGTGGCCCAGAGCTGGAGGGCAAGCTCCTTCATCTCCAAGCTATGAAAACTCACTTCACTCCCTGCA GTCTCGAATGGAAGACCGCTTAGACAGGCTGGATGATGCTATCCATGTGCTACGAAACCATGCAGTTGGA CCTTCTACCAGTCTGCCTACTAGCCACAGTGACATACACAGTTTGCTGGGACCATCCCATAATGCATCAA TTGGAAACCTCAATTCAAACTATGGAGGATCCAGCCTTGTTACAAATAGTCGATCAGCTTCGATGGTCGG AACACATCGGGAAGATTCAGTCAGTCTCAATGGCAATCATTCGGTCCTGTCTAGTACTGTTGCTGCCTCA AACACAGAACTGAACCATAAAACACCAGAAAATTTCAGAGGTGGTGTACAAAATCAGTCTGGAAGTGTTG TTCCAACAGAAATCAAGACTGAAAACAAAGAAAAAGATGAAAACCTTCATGAACCTCCTTCATCAGATGA CATGAAATCAGATGATGAGTCCTCCCAGAAAGACATCAAGGTCTCATCTAGGGGCAGAACAAGCAGTACC AATGAAGACGAGGATCTGAATCCAGAACAGAAAATCGAAAGGGAGAAGGAAAGGCGGATGGCTAACAATG CCAGAGAGCGCCTGCGCGTGCGGGATATTAACGAGGCGTTCAAGGAGCTTGGCCGAATGTGTCAGCTTCA TTTGAAGAGTGAAAAACCTCAGACAAAACTTCTCATTCTTCATCAGGCCGTGGCAGTCATCCTTAGTCTA GAACAGCAAGTGAGAGAGAGGAACCTCAACCCCAAAGCAGCCTGCCTTAAGAGAAGAGAAGAAGAAAAAG TCTCTGCTGCGTCAGCAGAGCCGCCCAACACGTTGCCAGGAGCCCATCCTGGGCTTAGTGAGTCTACCAA CCCTATGGGTCATCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230872 representing NM_001253862
Red=Cloning site Green=Tags(s) MNPQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPTTLGSSQFSGSGMDERGGTTSWGTSGQPSPSY DSSRGFTDSPHYSDHLNDSRLGTHEGLSPTPFMNSNLIGKTSERGSFSLYSRDSGLSGCQSSLLRQDLGL GSPAQLSSSGKPGTPYYSFSATSSRRRPLHDSVALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFNRESPS YPSPKPPTSMFASTFFMQDGTHSSSDLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGSLHSHDRLSYPPH SVSPTDINTSLPPMSSFHRGSTSSSPYVAASHTPPINGSDSILGTRGNAAGSSQTGDALGKALASIYSPD HTSSSFPSNPSTPVGSPSPLTGTSQWPRAGGQAPSSPSYENSLHSLQSRMEDRLDRLDDAIHVLRNHAVG PSTSLPTSHSDIHSLLGPSHNASIGNLNSNYGGSSLVTNSRSASMVGTHREDSVSLNGNHSVLSSTVAAS NTELNHKTPENFRGGVQNQSGSVVPTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSST NEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSL EQQVRERNLNPKAACLKRREEEKVSAASAEPPNTLPGAHPGLSESTNPMGHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001253862 |
ORF Size | 2046 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001253862.1, NP_001240791.1 |
RefSeq Size | 4578 bp |
RefSeq ORF | 2049 bp |
Locus ID | 21406 |
MW | 73.4 kDa |
Gene Summary | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228661 | Tcf12 (untagged) - Mouse transcription factor 12 (Tcf12), transcript variant 2 |
CNY 6,560.00 |