Amph (NM_001289546) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230895 representing NM_001289546 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACATCAAGACGGGCATCTTCGCCAAAAACGTGCAAAAGCGCCTCAACCGCGCGCAGGAAAAGG TCCTCCAGAAGCTGGGGAAGGCTGACGAGACAAAAGATGAACAGTTTGAAGAATATGTCCAGAACTTCAA ACGGCAGGAGGCAGAGGGTACCAGACTTCAGCGAGAACTCCGGGGATATTTAGCAGCAATCAAAGGTATG CAGGAGGCCTCAATGAAGCTCACTGAATCTCTACATGAAGTCTACGAGCCAGACTGGTATGGACGGGAAG ATGTAAAGATGGTTGGTGAGAAGTGTGATGTGCTGTGGGAAGACTTCCATCAAAAGCTAGTGGATGGCTC CTTGCTGACACTGGACACCTACCTGGGCCAGTTTCCTGACATAAAGAATCGCATCGCTAAGCGCAGCAGG AAGCTTGTGGATTATGATAGTGCTCGCCATCACCTGGAGGCTCTGCAGAGTTCCAAACGGAAAGATGAGA GTCGGATCTCTAAGGCAGAAGAGGAGTTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTCAATGTTGACTT ACAAGAAGAGTTGCCGTCATTGTGGTCAAGTCGAGTTGGCTTTTACGTCAATACTTTCAAAAATGTCTCC AGCCTTGAGGCCAAGTTTCATAAGGAAATCGCAGTGCTTTGCCACAAGCTCTATGAAGTGATGACGAAAC TCGGTGATCAGCATGCTGACAAGGCCTTCAGCATTCAAGGAGCTCCCAGTGACTCGGGTCCTCTTCGCAT TGCAAAGACACCATCACCTCCAGAGGAGCCTTCTCCCCTGCCCAGCCCCACGGCTAGTCCCAACCACACA TTAGCACCTGCATCTCCTGCCCCAGTGCGACCCAGATCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC CACCTCTGCCTAAAGTCACCCCAACAAAGGAACTGAAACAGGAGAACATCATCAATTTCTTTGAGGACAA CTTTGTACCAGAAATCAATGTGACAACACCTTCCCAGAACGAAGTCTTGGAGGTGAAGAAGGAAGAGACT TTGCTGGATTTGGACTTTGACCCTTTCAAGCCCGATGTGGCTCCGGCAGGTTCTGCTGCAGCGACCCACT CTCCCATGTCTCAGACATTGCCCTGGGACTTATGGACGACAAGCACTGATTTGGTACAACCAGCTTCTGG GGGTTCATTTAATGATTTCACCCAGGCCCAGGACACTTCATTATTCACCATGCAGACAGATCAGAATATG GTAGGTGGCTTGGCTGAAACTGAGCAGGCTCTACCTACAGAGCCCCAAGCCGAAGAGCCTCCTGCCACTG CTGCTGCACCTACTGCTGGGCTGGACCTTGGGCTAGAGATGGAGGAGCCAAAGGAGGAGGCTGTGATCCC TCCCGCAACTGATACTGGCGAGACTGTGGAAACTGCAGTGCCAACTGAGGGTGCCCCGGTGGAGGAAGCA GAGGCAGAGAAGGCAGCCCTCCCTGCTGGAGAAGGAGGAAGTCCAGAGGGAGCCAAAATTGACGGGGAAA GCACGGAGCTTGCAATCAGTGAGAGCCCTCAACCAGTGGAGCCCGAAGCGGGTGCTCCTCAGGTCATCCC TTCTGTTGTCATTGAGCCAGCCTCCAACCATGAAGGAGAAGGAGAACACCAAGAAACTGCTACGGGTACT GAACCGAGGGAGGCCGCTGAGGATGTGGCTGCCCAGGGCTCAGCGGGTGAGAAGCAGGAAGTGGCTACAG AGCCCACGCCTCTGGATTCTCAGGCAACTCTGCCTGCATCTGCAGGTGCTGTGGATGCCTCGCTGTCTGC AGGGGATGCCACTCAGGAACTGCCTCCTGGCTTTCTCTACAAGGTGGAAACCCTGCATGATTTCGAGGCA GCGAACTCTGATGAACTTAACCTACAAAGGGGTGACGTGGTACTCGTGGTGCCTTCAGACTCAGAAGCCG ACCAGGATGCAGGCTGGTTGGTGGGGGTGAAGGAGTCAGACTGGCTTCAGTACAGAGACCTGGCCACGTA CAAAGGCCTCTTTCCAGAGAACTTCACGCGGCGCCTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230895 representing NM_001289546 Red=Cloning site Green=Tags(s) MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGM QEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSR KLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVS SLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHT LAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEET LLDLDFDPFKPDVAPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNM VGGLAETEQALPTEPQAEEPPATAAAPTAGLDLGLEMEEPKEEAVIPPATDTGETVETAVPTEGAPVEEA EAEKAALPAGEGGSPEGAKIDGESTELAISESPQPVEPEAGAPQVIPSVVIEPASNHEGEGEHQETATGT EPREAAEDVAAQGSAGEKQEVATEPTPLDSQATLPASAGAVDASLSAGDATQELPPGFLYKVETLHDFEA ANSDELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289546
ORF Size	2070 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289546.1, NP_001276475.1
RefSeq Size	3247 bp
RefSeq ORF	2073 bp
Locus ID	218038
UniProt ID	Q7TQF7
MW	75.8 kDa
Gene Summary	May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function]

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