Kif3a (NM_001290806) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Kif3; Kifl; Kns3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230909 representing NM_001290806 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATCAATAAGTCGGAGAAGCCGGAAAGCTGCGATAATGTGAAGGTGGTGGTTAGGTGCCGGCCTC TCAATGAGAGAGAGAAGTCAATGTGCTATAGACAGGCCGTCAGCGTGGATGAAATGAGGGGAACTATCAC CGTCCATAAGACCGATTCCTCCAACGAACCTCCAAAGACATTTACTTTCGATACTGTTTTTGGACCAGAA AGTAAACAACTTGATGTGTATAACTTAACTGCAAGACCAATTATTGATTCTGTTCTGGAAGGCTACAATG GGACCATCTTTGCATATGGACAGACTGGGACAGGCAAGACTTTCACCATGGAAGGCGTTCGAGCAGTACC GGGGCTCAGAGGGGTCATCCCCAACTCATTTGCTCACATATTTGGTCACATCGCAAAAGCAGAGGGCGAC ACAAGGTTTTTGGTTCGAGTGTCATACTTGGAGATATATAATGAAGAAGTTCGAGACCTTTTGGGTAAAG ATCAGACCCAGAGGTTAGAGGTTAAAGAAAGGCCTGATGTGGGAGTATATATCAAAGACTTATCAGCATA TGTAGTCAACAACGCTGACGACATGGATAGAATCATGACTCTAGGCCACAAAAACCGTTCTGTCGGTGCG ACTAATATGAACGAGCACAGCTCCCGATCCCACGCCATCTTTACAATTACTATAGAGTGCAGTGAGAAAG GCGTGGACGGGAACATGCACGTCAGGATGGGCAAGCTCCACCTTGTGGATCTGGCTGGGTCAGAAAGACA AGCAAAAACTGGAGCCACAGGACAGCGCCTCAAGGAAGCTACCAAAATCAACCTTTCATTGTCTACCCTG GGCAACGTTATTTCTGCCTTGGTGGATGGAAAAAGCACTCACGTGCCGTATCGGAACTCCAAACTGACCC GTCTCCTGCAGGACTCCTTAGGAGGGAACTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGATTA TAATTATGATGAAACTATCAGCACCTTGCGGTATGCCAACCGCGCCAAGAACATTAAAAACAAAGCGAGA ATCAATGAGGACCCAAAGGATGCTCTGCTCCGCCAGTTTCAGAAAGAAATCGAAGAGCTGAAGAAAAAGC TTGAGGAAGGGGAAGAAGTGTCAGGCTCTGACATCAGTGGGTCAGAGGAGGATGACGAGGAGGGTGAACT TGGAGAAGATGGAGAAAAAAAGAAAAAGAGAAGGGACCAAGCAGATAAGATGGTGGAAATGCAAGCGAAA ATTGACGAGGAGAGAAAAGCACTGGAAACCAAACTTGACATGGAAGAAGAAGAAAGAAACAAGGCTAGAG CTGAACTAGAGAGACGGGAGAAGGACCTTCTGAAAGCCCAACAAGAGCATCAGTCTTTGCTGGAAAAGCT GTCTGCTTTGGAAAAGAAGGTCATTGTCGGCGGCGTGGACCTGCTGGCCAAGGCTGAGGAGCAAGAGAAG CTTCTGGAGGAGTCCAACATGGAGCTGGAGGAGAGGAGGAGACGAGCTGAGCAGCTTCGGAAAGAGCTGG AGGAAAAGGAGCAAGAACGCTTGGACATCGAGGAAAAATATACCAGTCTGCAAGAGGAGGCACAAGGAAA GACCAAGAAATTAAAGAAAGTCTGGACCATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTCCAGCAG GAGCATCAGAGGGAGATCGAAGGCCTCCTGGAGAACATCCGGCAGCTCAGCCGGGAGCTCCGTCTTCAGA TGCTCATCATTGATAACTTCATACCTCAGGATTACCAGGAAATGATTGAAAACTACGTCCACTGGAATGA AGACATAGGAGAATGGCAGCTAAAATGTGTGGCCTACACAGGAAATAACATGAGGAAACAAACTCCAGTC CCTGACAAGAAGGAGAGAGACCCATTTGAAGTCGACCTTTCCCACGTGTACCTCGCCTATACAGAGGAGA GTCTGCGTCAGTCTCTGATGAAGTTAGAAAGGCCACGGACCTCCAAGGGGAAAGCAAGGCCAAAGATGGG GCGAAGAAAGCGTTCTGCAAAGCCTGAGACCGTAATTGATTCTTTACTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230909 representing NM_001290806 Red=Cloning site Green=Tags(s) MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYRQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPE SKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPGLRGVIPNSFAHIFGHIAKAEGD TRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGA TNMNEHSSRSHAIFTITIECSEKGVDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTL GNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKAR INEDPKDALLRQFQKEIEELKKKLEEGEEVSGSDISGSEEDDEEGELGEDGEKKKKRRDQADKMVEMQAK IDEERKALETKLDMEEEERNKARAELERREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEK LLEESNMELEERRRRAEQLRKELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQ EHQREIEGLLENIRQLSRELRLQMLIIDNFIPQDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPV PDKKERDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKMGRRKRSAKPETVIDSLLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290806
ORF Size	2082 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290806.1, NP_001277735.1
RefSeq Size	5429 bp
RefSeq ORF	2085 bp
Locus ID	16568
UniProt ID	P28741
MW	79.9 kDa
Gene Summary	Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (PubMed:21670265). Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole (PubMed:23386061).[UniProtKB/Swiss-Prot Function]

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