Larp4 (NM_001284522) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DXErtd793; DXErtd793e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230969 representing NM_001284522 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTGGAGGTGACATCTAAAGGCACTGGTTTAAATCCTAATGCCAAAGTATGGCAAGAAATTCCTT CTGGAAATCCGGATGGCACTCCTGTAACTGAACCCTCTTGGCATGAAACTGCAGCCACATCTGGATCTCA CCCTGAGGGCCATACAGAACTTTCAGAAGATATGTGTAAGGAATATGAAGTAATGTATTCTCCATCTTGT GAAACCACAAGGAATACTGCAGATGTTGAAGAGTCAGCTGATGGAATGATCTTAGGACCGGAAGACCTGA GTTACCAACTCTATGATGTTTCTGGAGAAAGCAGTTCAGCAATTTCTACAGAAGATTTAAAAGAATGTCT GAAAAAACAATTGGAATTCTGTTTCTCACGAGAAAATTTATCAAAGGACCTTTACTTGATATCTCAAATG GATAGTGATCAGTTCGTCCCAATATGGACGGTTGCCAACATGGAAGAAATTAAAAAATTGACTACAAATA CAGATTTAATTCTTGAAGTATTGAGATCTTCCCCTATGGTACAAGTTGATGAGAAGGGAGAAAAGGTGAG ACCAAGTCATAAGCGATGTATTGTGATTCTCAGAGAGATTCCTGAGACAACGCCTGTAGAGGAAGTGAAA GCTTTGTTTAAAAATGAAAACTGCCCCAAAGTGATAAGCTGTGAGTTTGCGCACAACAGCAACTGGTACA TCACTTTCCAGTCAGACACAGATGCACAGCAGGCTTTTAAATACTTAAGAGAAGAAGTTAAAACATTTCA GGGCAAGCCAATCATGGCAAGGATAAAAGCCATCAATACATTTTTTGCTAAGAATGGTTATCGATTAATG GATTCTAGTATGTATACTCAACCTATTCAGACTCCTACCCAATATCCCTCACCAGTCTTCATGCAACCAG TGTATAATCCTCAGCAGTACTCAGTGTATAGTCTTGTGCCTCAGTCTTGGTCTCCAAGTCCTGCACCTTA CTTTGAAACACCACTGGCTCCCTTTCCCAATGGTAGTTTTGTGAATGGCTTTAGTTCACCAGGATCTTAC AAAACAAATGCTGCTGCTATGAATATGGGCCGACCATTCCCAAAAAATCGTGTGAAGCCTCATTTTAGGT CATCCAGTGGTTCAGAACACTCAACAGAGGGCTCCGTGTCATTGGGGGATGGACCACTGAGCAGATCTAG TTCAAGGATCTTTCTTTCTGAACGGCATAACCCAACAGTAACAGGGCAGCAGGAACAAACTTATCTTCCA AAGGAGGCTCCCATTTTACAGATGGAACAGAATGGGGACTTTGGAAGAGGCAGGCGAACTCTCTTCAGAG GCCGAAGGCGAAGGGATGATGACAGGATCCCAAGACCCCAACCTGCAGCAACTGAAGCCAAGGCTCCAAC TCCGAAGTTTGATTTGCTTGCTACAAATTTCCCTCCTTTACCTGGAAGCTCATCGAGAGTGCCAGATGAA CTTGGTTTGGAGAACAGAATGTCTGATGTCGTTAAAGGTGTCTGCAGAGAAAAGGACAGCGAGGACGTGC GAGTTAGTTGCCCAGTGCCTGCAGAGGACGGACAGACAGACTGCACTTCTGCGCCGCTCAGCATAAGTCC TAGTCCTCCGTGTACAGCTGAGCCTCCTGTGTTAAGCACGACTCAGCAAGAGCAGGATCAGATGGAGGAC TCTGCTGTTCCAAAGGACACTCTCAACCCAGTAGCTGTGCCAGTGTCTTCTCCAACTGCCACAAAGCCAT CCCCGGCAAACACGGCTTCACCTTGCACTAGTAACATAAACCCACCGAGAGCTGTGGCCCTGCAGGAACC TCGAAAGTTAAGCTATGCTGAAGTGTGCCAGAAGCCTCCTAAAGAGCCATCTCCAGTTCTCGTACAGCCG CTGCGGGAACTCCGGTCTAATGCAGTATCCCCCACCAGAAATGAAGAGAACGGAGCTCCCGAGAAGCCTG TTGAGAAGCCGCATGAGAAACCAGAAACAAGGGCTAGTAAGGATCATTCTGGCTTCCGAGGCAACACTAT TCCCAGGGGAGCGGCTGGAAAAATCAGGGAACAGAGACGCCAGTTCAGCCATAGGGCTACACCTCAGGGA GTGACTCGACGTAATGGGAAAGAGCAATACGTGCCACCCAGATCACCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230969 representing NM_001284522 Red=Cloning site Green=Tags(s) MALEVTSKGTGLNPNAKVWQEIPSGNPDGTPVTEPSWHETAATSGSHPEGHTELSEDMCKEYEVMYSPSC ETTRNTADVEESADGMILGPEDLSYQLYDVSGESSSAISTEDLKECLKKQLEFCFSRENLSKDLYLISQM DSDQFVPIWTVANMEEIKKLTTNTDLILEVLRSSPMVQVDEKGEKVRPSHKRCIVILREIPETTPVEEVK ALFKNENCPKVISCEFAHNSNWYITFQSDTDAQQAFKYLREEVKTFQGKPIMARIKAINTFFAKNGYRLM DSSMYTQPIQTPTQYPSPVFMQPVYNPQQYSVYSLVPQSWSPSPAPYFETPLAPFPNGSFVNGFSSPGSY KTNAAAMNMGRPFPKNRVKPHFRSSSGSEHSTEGSVSLGDGPLSRSSSRIFLSERHNPTVTGQQEQTYLP KEAPILQMEQNGDFGRGRRTLFRGRRRRDDDRIPRPQPAATEAKAPTPKFDLLATNFPPLPGSSSRVPDE LGLENRMSDVVKGVCREKDSEDVRVSCPVPAEDGQTDCTSAPLSISPSPPCTAEPPVLSTTQQEQDQMED SAVPKDTLNPVAVPVSSPTATKPSPANTASPCTSNINPPRAVALQEPRKLSYAEVCQKPPKEPSPVLVQP LRELRSNAVSPTRNEENGAPEKPVEKPHEKPETRASKDHSGFRGNTIPRGAAGKIREQRRQFSHRATPQG VTRRNGKEQYVPPRSPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284522
ORF Size	2151 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284522.1, NP_001271451.1
RefSeq Size	6550 bp
RefSeq ORF	2154 bp
Locus ID	207214
UniProt ID	Q8BWW4
MW	79.9 kDa
Gene Summary	RNA binding protein that binds to the poly-A tract of mRNA molecules. Associates with the 40S ribosomal subunit and with polysomes. Plays a role in the regulation of mRNA translation. Plays a role in the regulation of cell morphology and cytoskeletal organization.[UniProtKB/Swiss-Prot Function]

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