Atp6v0a1 (NM_001243049) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA959968; ATP6a1; Atp6n1; Atp6n1a; Atpv0a1; Vpp-1; Vpp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231133 representing NM_001243049 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGAAAACTCCGATTTGTTGAGAAAGAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTG AAAACCCAGAGGTGCCCTTCCCTCGGGACATGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGA ACTGAAGGAAATCAACACTAACCAGGAAGCTCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTT ATCCTGCGAAAAACCCAGCAGTTTTTCGATGAGCAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCAT CACTCTTGGAGCCAAACGAGATGGGAAGAGGCGCACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAA CCGGGAGCGGATCCCGACCTTTGAGCGCATGCTTTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAA GCTGAAATCGAAAACCCGCTGGAGGATCCTGTGACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTT TTTTCCAAGGCGATCAGCTGAAAAACAGGGTCAAGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCC CTGCCCTGAGACTCCACAGGAAAGGAAAGAAATGGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAA ATGGTTCTGAATCAGACGGAGGACCACCGCCAGAGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCT GGTTCATCAAGGTGCGGAAGATGAAGGCTATCTACCATACCCTGAACCTGTGCAATATAGACGTGACCCA GAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGTCACTGACCTGGACTCCATCCAGTTTGCACTACGAAGA GGCACGGAACACAGTGGGTCCACTGTCCCCTCCATTCTGAACAGGATGCAGACAAACCAGACACCGCCGA CATATAATAAAACCAACAAGTTCACGCACGGCTTCCAGAACATAGTAGATGCTTATGGGATCGGCACTTA CCGAGAGATTAACCCAGCTCCGTACACTGTCATCACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGAT TTTGGGCACGGCATTTTGATGACGCTGTTTGCTGTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCC AGAAGCACGAGAATGAGATGTTTAGCATGGTGTTCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTT CTCCATCTATACTGGACTCATCTACAATGACTGCTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGG AGTGTACGGCCAATGTTCACTCAAGGGAACTGGACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGT TGAACCCCGCTATCCCTGGAGTTTTTGGTGGCCCGTACCCGTTTGGCATTGATCCGATTTGGAACATTGC AACCAACAAGCTGACCTTCCTCAACTCCTTCAAGATGAAGATGTCTGTTATCCTCGGCATCATCCACATG CTGTTTGGAGTCAGCCTGAGCCTTTTCAACCATATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCT TTATTCCTGAGATCATCTTCATGTCCTCGTTGTTTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGAC AGCCTACGATGCCCACTCGTCTAGGAATGCCCCGAGCCTCCTGATCCACTTCATAAACATGTTCCTCTTC TCCTACCCAGAGTCTGGTAATGCAATGCTGTACTCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGG TGGCGATGCTCTGCGTCCCTTGGATGCTTCTGTTTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAA GAAGCATTTGGGAACTCTCAACTTTGGTGGGATCAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAA ATTATTCAGCATGACCAGCTTTCCACCCATTCAGAGGACGCAGAAGAGCCTACCGAGGACGAAGTGTTTG ACTTTGGCGACACCATGGTCCACCAGGCCATCCACACCATTGAGTACTGCTTGGGTTGTATCTCCAACAC CGCGTCGTACCTGCGACTCTGGGCCCTCAGCCTGGCCCACGCACAGCTCTCTGAAGTACTTTGGACCATG GTGATCCACATTGGCCTGCACGTCCGGAGTTTGGCCGGGGGTCTAGGGCTGTTTTTCATCTTCGCCGCCT TTGCCACGCTGACCGTGGCTATCCTGCTGATCATGGAGGGCCTCTCGGCCTTCCTCCACGCGCTGCGGTT ACACTGGGTGGAGTTCCAGAACAAATTCTACACTGGGACTGGCTTCAAGTTCCTCCCCTTCTCCTTTGAG CACATTCGGGAAGGGAAGTTTGATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231133 representing NM_001243049 Red=Cloning site Green=Tags(s) MDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKF ILRKTQQFFDEQMADPDLLEESSSLLEPNEMGRGAPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQ AEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQ MVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRR GTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTHGFQNIVDAYGIGTYREINPAPYTVITFPFLFAVMFGD FGHGILMTLFAVWMVLRESRILSQKHENEMFSMVFSGRYIILLMGLFSIYTGLIYNDCFSKSLNIFGSSW SVRPMFTQGNWTEETLLGSSVLQLNPAIPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHM LFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMSSLFGYLVILIFYKWTAYDAHSSRNAPSLLIHFINMFLF SYPESGNAMLYSGQKGIQCFLIVVAMLCVPWMLLFKPLILRHQYLRKKHLGTLNFGGIRVGNGPTEEDAE IIQHDQLSTHSEDAEEPTEDEVFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTM VIHIGLHVRSLAGGLGLFFIFAAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYTGTGFKFLPFSFE HIREGKFDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243049
ORF Size	2337 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243049.2
RefSeq Size	3841 bp
RefSeq ORF	2340 bp
Locus ID	11975
MW	89.9 kDa
Gene Summary	Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function]

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