Eml1 (NM_001286347) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231174 representing NM_001286347 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGTCAGACCGCATCGCCTCTCTGGAGCAGCGCGTCCAGATGCAGGAGGATGACATTCAACTGC TCAAGTCAGCGCTGGCCGACGTGGTTCGGAGGCTGAACATCACGGAGGAGCAACAGGCTGTGCTCAACAG GAAAGGACCTACCAAAGCGAGGCCACTGGGGCAGACCCTGCCATTGAGAACCACCGTCAACAATGGCACC GTGTTACCAAAGAAACCCAGTGCCTCCCTCCCGGCACCCTCCGGGGCCAGGAAAGAAGTAGTTGTGCCGG TAACCAAAAGCATCAATAGGACCAGCTCTTCCGAAAGAGTGTCTCCAGGTGGCCGGAGGGAGAGCTCTGG GGACTCCAAAGGAAGCCGGAACCGCACGGGCTCAACCAGTAGCTCCTCCAGCGGCAAGAAGAACAGTGAG AGCAAACCCAAGGAGCCCGCATTCAGTCCAGCCCTTCAGTCTCCGAAACCCCAAGGGAAGCGCCGGGTGA CACACTGCAAAGAAGAAGGATATGTAAAAATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCCCAA AGACCAAGTGGATTCGTACAGTTTGGAAGCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAGTGG GTCTACGGGTACCGGGGGCGAGACTGTCGCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGTACT TCATTGCGTCTGTCGTGGTGCTTTACAATGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAACGA TGACGTCAAGTGCCTAGCGGTCCATCCTGACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACATCT AAGGATGGAAAGCAACTGCCACCACATGTGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATGTCA TTGGAATAGGCTTTTTTGACCGGGCTGTCACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCATCT CTGTGCTGTGGATGATTCCAACGATCACGTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTGGCC GACGTGAAGTGTTCGAACGAAGCGGTATTTGCGGCGGACTTCCACCCCACAGACACCAATATCATAGTCA CCTGCGGGAAGTCACATCTCTACTTTTGGACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTTGTT TGAGAAACAAGAGAAGCCAAAGTTTGTTCTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACTGGA GATTCAAGCGGCAACATCTTGGTGTGGGGGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGGCCC ACGAGGGCGGCATTTTTGCACTTTGTATGCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGACCG GCGACTCATTTCCTGGAACGGAAACTATCAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGCCCA ATACGGACAGTAGCCGAAGGGAAGGGCAACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGCAAG GCACCTTGTCAGGGGACTTCACACCCATCACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCATCCA TGCCTCCAAGCCTCAGTTCCTGACTTGTGGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGTCAC CGGCCAGTCTGGGACAAAATCATAGAGGATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTGTGG TTGCAGTGGGGACACTGACTGGGAGGTGGTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGTCCA CACGGATGGGAATGAGCAGCTGTCCGTGATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGCTCC CATGACAACTGCATCTACATATATGGAGTTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGTGCT CCGGCCATTCCAGCTTCATCACCCACTTGGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTCCGG GGACTACGAGATCCTCTACTGGGTTCCGTCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGGGAC ATCGAGTGGGCCACCTATACCTGCACCTTGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCGATG GGACAGACATCAACGCCGTCTGCCGGGCTCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGGCAA AGTGCACCTCTTCTCATACCCGTGCTCACAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGCAGC CACGTCACCAACGTGGACTTCCTCTGTGAAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAAGCA TCATGCAGTGGCGAGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231174 representing NM_001286347 Red=Cloning site Green=Tags(s) MEVSDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLGQTLPLRTTVNNGT VLPKKPSASLPAPSGARKEVVVPVTKSINRTSSSERVSPGGRRESSGDSKGSRNRTGSTSSSSSGKKNSE SKPKEPAFSPALQSPKPQGKRRVTHCKEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKAELPTKRLKLEW VYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTS KDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGGHLCAVDDSNDHVLSVWDWQKEERLA DVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGNSLNKKQGLFEKQEKPKFVLCVTFSENGDTITG DSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRRLISWNGNYQKLHKAEIPEQFGP IRTVAEGKGNVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKPQFLTCGHDKHATLWDAVGH RPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPDGNFLAIGS HDNCIYIYGVTDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWVPSACKQVVSVETTRD IEWATYTCTLGFHVFGVWPEGSDGTDINAVCRAHERKLLCTGDDFGKVHLFSYPCSQFRAPSHIYSGHSS HVTNVDFLCEDSHLISTGGKDTSIMQWRVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286347
ORF Size	2400 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286347.1, NP_001273276.1
RefSeq Size	3857 bp
RefSeq ORF	2403 bp
Locus ID	68519
UniProt ID	Q05BC3
MW	88.8 kDa
Gene Summary	Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function]

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