Eml1 (NM_001286346) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231178 representing NM_001286346 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACGGCGAGGGCCCCTCAGCTGATGACAGCGCGTCTGCTGCCAGCAGCATGGAGGTGTCAGACC GCATCGCCTCTCTGGAGCAGCGCGTCCAGATGCAGGAGGATGACATTCAACTGCTCAAGTCAGCGCTGGC CGACGTGGTTCGGAGGCTGAACATCACGGAGGAGCAACAGGCTGTGCTCAACAGGAAAGGACCTACCAAA GCGAGGCCACTGGGGCAGACCCTGCCATTGAGAACCACCGTCAACAATGGCACCGTGTTACCAAAGAAAC CCAGTGCCTCCCTCCCGGCACCCTCCGGGGCCAGGAAAGAAGTAGTTGTGCCGGTAACCAAAAGCATCAA TAGGACCAGCTCTTCCGAAAGAGTGTCTCCAGGTGGCCGGAGGGAGAGCTCTGGGGACTCCAAAGGAAGC CGGAACCGCACGGGCTCAACCAGTAGCTCCTCCAGCGGCAAGAAGAACAGTGAGAGCAAACCCAAGGAGC CCGCATTCAGTCCAGAAGAAGGATATGTAAAAATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCC CAAAGACCAAGTGGATTCGTACAGTTTGGAAGCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAG TGGGTCTACGGGTACCGGGGGCGAGACTGTCGCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGT ACTTCATTGCGTCTGTCGTGGTGCTTTACAATGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAA CGATGACGTCAAGTGCCTAGCGGTCCATCCTGACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACA TCTAAGGATGGAAAGCAACTGCCACCACATGTGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATG TCATTGGAATAGGCTTTTTTGACCGGGCTGTCACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCA TCTCTGTGCTGTGGATGATTCCAACGATCACGTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTG GCCGACGTGAAGTGTTCGAACGAAGCGGTATTTGCGGCGGACTTCCACCCCACAGACACCAATATCATAG TCACCTGCGGGAAGTCACATCTCTACTTTTGGACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTT GTTTGAGAAACAAGAGAAGCCAAAGTTTGTTCTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACT GGAGATTCAAGCGGCAACATCTTGGTGTGGGGGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGG CCCACGAGGGCGGCATTTTTGCACTTTGTATGCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGA CCGGCGACTCATTTCCTGGAACGGAAACTATCAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGC CCAATACGGACAGTAGCCGAAGGGAAGGGCAACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGC AAGGCACCTTGTCAGGGGACTTCACACCCATCACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCAT CCATGCCTCCAAGCCTCAGTTCCTGACTTGTGGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGT CACCGGCCAGTCTGGGACAAAATCATAGAGGATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTG TGGTTGCAGTGGGGACACTGACTGGGAGGTGGTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGT CCACACGGATGGGAATGAGCAGCTGTCCGTGATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGC TCCCATGACAACTGCATCTACATATATGGAGTTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGT GCTCCGGCCATTCCAGCTTCATCACCCACTTGGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTC CGGGGACTACGAGATCCTCTACTGGGTTCCGTCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGG GACATCGAGTGGGCCACCTATACCTGCACCTTGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCG ATGGGACAGACATCAACGCCGTCTGCCGGGCTCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGG CAAAGTGCACCTCTTCTCATACCCGTGCTCACAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGC AGCCACGTCACCAACGTGGACTTCCTCTGTGAAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAA GCATCATGCAGTGGCGAGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231178 representing NM_001286346 Red=Cloning site Green=Tags(s) MSDGEGPSADDSASAASSMEVSDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTK ARPLGQTLPLRTTVNNGTVLPKKPSASLPAPSGARKEVVVPVTKSINRTSSSERVSPGGRRESSGDSKGS RNRTGSTSSSSSGKKNSESKPKEPAFSPEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKAELPTKRLKLE WVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGT SKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGGHLCAVDDSNDHVLSVWDWQKEERL ADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGNSLNKKQGLFEKQEKPKFVLCVTFSENGDTIT GDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRRLISWNGNYQKLHKAEIPEQFG PIRTVAEGKGNVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKPQFLTCGHDKHATLWDAVG HRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPDGNFLAIG SHDNCIYIYGVTDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWVPSACKQVVSVETTR DIEWATYTCTLGFHVFGVWPEGSDGTDINAVCRAHERKLLCTGDDFGKVHLFSYPCSQFRAPSHIYSGHS SHVTNVDFLCEDSHLISTGGKDTSIMQWRVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286346
ORF Size	2403 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286346.1, NP_001273275.1
RefSeq Size	3943 bp
RefSeq ORF	2406 bp
Locus ID	68519
UniProt ID	Q05BC3
MW	88.5 kDa
Gene Summary	Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function]

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