Eml1 (NM_001286346) Mouse Tagged ORF Clone
CAT#: MR231178
- TrueORF®
Eml1 (myc-DDK-tagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 3
"NM_001286346" in other vectors (1)
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CNY 7,980.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231178 representing NM_001286346
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACGGCGAGGGCCCCTCAGCTGATGACAGCGCGTCTGCTGCCAGCAGCATGGAGGTGTCAGACC GCATCGCCTCTCTGGAGCAGCGCGTCCAGATGCAGGAGGATGACATTCAACTGCTCAAGTCAGCGCTGGC CGACGTGGTTCGGAGGCTGAACATCACGGAGGAGCAACAGGCTGTGCTCAACAGGAAAGGACCTACCAAA GCGAGGCCACTGGGGCAGACCCTGCCATTGAGAACCACCGTCAACAATGGCACCGTGTTACCAAAGAAAC CCAGTGCCTCCCTCCCGGCACCCTCCGGGGCCAGGAAAGAAGTAGTTGTGCCGGTAACCAAAAGCATCAA TAGGACCAGCTCTTCCGAAAGAGTGTCTCCAGGTGGCCGGAGGGAGAGCTCTGGGGACTCCAAAGGAAGC CGGAACCGCACGGGCTCAACCAGTAGCTCCTCCAGCGGCAAGAAGAACAGTGAGAGCAAACCCAAGGAGC CCGCATTCAGTCCAGAAGAAGGATATGTAAAAATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCC CAAAGACCAAGTGGATTCGTACAGTTTGGAAGCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAG TGGGTCTACGGGTACCGGGGGCGAGACTGTCGCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGT ACTTCATTGCGTCTGTCGTGGTGCTTTACAATGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAA CGATGACGTCAAGTGCCTAGCGGTCCATCCTGACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACA TCTAAGGATGGAAAGCAACTGCCACCACATGTGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATG TCATTGGAATAGGCTTTTTTGACCGGGCTGTCACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCA TCTCTGTGCTGTGGATGATTCCAACGATCACGTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTG GCCGACGTGAAGTGTTCGAACGAAGCGGTATTTGCGGCGGACTTCCACCCCACAGACACCAATATCATAG TCACCTGCGGGAAGTCACATCTCTACTTTTGGACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTT GTTTGAGAAACAAGAGAAGCCAAAGTTTGTTCTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACT GGAGATTCAAGCGGCAACATCTTGGTGTGGGGGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGG CCCACGAGGGCGGCATTTTTGCACTTTGTATGCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGA CCGGCGACTCATTTCCTGGAACGGAAACTATCAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGC CCAATACGGACAGTAGCCGAAGGGAAGGGCAACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGC AAGGCACCTTGTCAGGGGACTTCACACCCATCACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCAT CCATGCCTCCAAGCCTCAGTTCCTGACTTGTGGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGT CACCGGCCAGTCTGGGACAAAATCATAGAGGATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTG TGGTTGCAGTGGGGACACTGACTGGGAGGTGGTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGT CCACACGGATGGGAATGAGCAGCTGTCCGTGATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGC TCCCATGACAACTGCATCTACATATATGGAGTTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGT GCTCCGGCCATTCCAGCTTCATCACCCACTTGGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTC CGGGGACTACGAGATCCTCTACTGGGTTCCGTCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGG GACATCGAGTGGGCCACCTATACCTGCACCTTGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCG ATGGGACAGACATCAACGCCGTCTGCCGGGCTCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGG CAAAGTGCACCTCTTCTCATACCCGTGCTCACAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGC AGCCACGTCACCAACGTGGACTTCCTCTGTGAAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAA GCATCATGCAGTGGCGAGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231178 representing NM_001286346
Red=Cloning site Green=Tags(s) MSDGEGPSADDSASAASSMEVSDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTK ARPLGQTLPLRTTVNNGTVLPKKPSASLPAPSGARKEVVVPVTKSINRTSSSERVSPGGRRESSGDSKGS RNRTGSTSSSSSGKKNSESKPKEPAFSPEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKAELPTKRLKLE WVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGT SKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGGHLCAVDDSNDHVLSVWDWQKEERL ADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGNSLNKKQGLFEKQEKPKFVLCVTFSENGDTIT GDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRRLISWNGNYQKLHKAEIPEQFG PIRTVAEGKGNVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKPQFLTCGHDKHATLWDAVG HRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPDGNFLAIG SHDNCIYIYGVTDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWVPSACKQVVSVETTR DIEWATYTCTLGFHVFGVWPEGSDGTDINAVCRAHERKLLCTGDDFGKVHLFSYPCSQFRAPSHIYSGHS SHVTNVDFLCEDSHLISTGGKDTSIMQWRVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286346 |
ORF Size | 2403 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286346.1, NP_001273275.1 |
RefSeq Size | 3943 bp |
RefSeq ORF | 2406 bp |
Locus ID | 68519 |
UniProt ID | Q05BC3 |
MW | 88.5 kDa |
Gene Summary | Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228967 | Eml1 (untagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 3 |
CNY 7,700.00 |