Gripap1 (NM_001290455) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI854681; DXImx47e; GRASP-1; mKIAA1167; Sfc10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231233 representing NM_001290455 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAAGCTCTGTCTGAAGAGGAGTTTCAACGGATGCAGACTCAGCTCCTGGAGCTCCGGACAAATA ACTACCAGCTTTCGGATGAACTTCGGAAGAATGGTGTTGAACTTTCCAGTCTTCGACAGAAGGTCGCCTA CCTGGATAAGGAGTTCAGTAAAGCTCAAAAGGCACTAAGCAAGAGCAAGAAAGCTCAGGAAGTGGAGGTA CTGCTGAGTGAAAATGAGATGCTGCAGGCAAAGCTGCACAGCCAGGAGGAGGACTTCCGTTTGCAGAACA GTACACTTATGGCCGAGTTCAGCAAGCTTTGCAGCCAGCTGGAGCAGCTGGAGCTGGAAAACCGACAACT CAAGGAAGGCGTTCCTGGAGCAGCTGGAGCACATGTGGACGGGGAGCTGCTGAGGCTGCAGGCAGAGAAC ACAGCCTTGCAGAAGAACATGGCAGCACTGCAGGAACGCTATGGGAAAGAGGCTGTGAGGCCCTCTGCTG TTGGTGAGGGCCAAGGGGATCCCCCAGGAGACGTACTCCCCACTCCCCTGGCTCCCATGCCATTGGCAGA AGTGGAGCTGAAATGGGAAATGGAGCGAGAAGAAAAAAAATTGCTCTGGGAGCAGCTACAAGGCTTAGAG AGCTCAAAGCAAGCTGAAACCTCCAGACTACAGGAGGAACTTGCTAAGCTCTCTGAGAAATTGAAAAAGA AACAAGAAAGTTTTTGCCGTCTGCAGACAGAGAAGGAAACACTGTTTAATGACAGCAGAAACAAAATTGA AGAATTGCAACAGCGGAAGGAAGCTGATCTGAAAGCCCAGTTGGCTCGCACCCAAAAACTACAGCAGGAA CTTGAGGCTGCCAATCAGAGTTTGGCAGAGCTAAGAGATCAGCGGCAGGGGGAGCGTCTGGAGCATGCAG CAGCGTTGCGGGCCCTACAAGATCAGGTGTCCAGCCAGAGTGCAGATGCGCAGGAACAAGTGGAAGGGCT CTTGGCTGAGAACAGTGCCTTGAGGACTAGCTTGGCTGCTCTGGAGCAGATCCAGACAGCAAAGACACAA GAACTGAATATGCTTCGGGAACAGACCAGTGAATTGGCATCTGAGTTACAGCATCGTCAAGCTGAATATG AAGAGCTTATGGGACAGAAAGATGACCTCAACTCGCAGCTCCAGGAGTCGTTAAGGGCCAATAGCCGGCT GCTGGAACAACTTCAAGAAATAGGGCAGGAGAAGGAGCAGCTAACCCAGGATCTCCAGGAAGCTCGGAAG AGTGCCGAGAAAAGGAAGGTCATGCTGGATGAACTAGCCATGGAGACGCTGCAGGAGAAGTCCCAGCACA AAGAAGAACTGGGGGCCGTCCGGTTACGGCACGAGAAGGAGCTGCTGGGCGTGCGAGCCCGATACGAGCG TGAGCTTCGAGAACTGCACGAGGACAAGAAGCGGCAGGAGGAGGAGCTCCGGGGGCAGATTCGTGAGGAG AAGGCTCGAACAAGGGAATTAGAAAATCTTCAGCACACAGTGGAAGAACTCCAGGCTCAGGTACACTCCA TGGATGGAGCCAAGGGCTGGTTTGAACGGCGCTTGAAGGAAGCTGAGGAATCTTTGCAGCAGCAGCAACA GGAACAAGAGGAAACCCTCAAGCTGTGCCGGGAGGAGCACGCCGCTGAGCTGAAGGGCAAGGATGAAGAG CTTCAGAATGTTCGGGAACAGCTCCAGCAGGCCCAGGAGGAGCGAGATGGCCACGTGAAGACCATCAGCA ACCTGAAGCAGGAGGTAAAGGACACAGTAGACGGGCAGCGCATCCTGGAGAAGAAAGGCAGCGCTGTGCT CAAAGATCTTAAGCGGCAGCTGCACTTGGAGCGGAAACGGGCTGATAAGCTGCAGGAACGGCTGCAGGAG ATCCTTACCAACAGCAAGAGCCGCACAGGCCTCGAGGAGCTGGTTCTGTCTGAGATGAACTCACCAAGCA GGACCCAGACAGGGGACAGCAGTAGTGTCTCCTCCTTCAGCTACCGGGAGATCTTGAAGGAGAAGGAGAG TTCAGCCATCCCAGCCAGGTCTCTATCCAGCAGTCCTCAGGCCCAGCCCCCTCGGCCAGCAGAGCTGTCA GATGAGGAAGTGGCTGAGCTGTTTCAGAGGCTGGCCGAGACTCAGCAGGAAAAATGGATGCTAGAGGAGA AGGTGAAACACCTGGAGGTGAGCAGCGCCTCTATGGCGGAAGATCTCTGCCGGAAAAGCGCCATCATTGA GACCTACGTCATGGACAGCCGGATTGATGTGTCTGTGGCAGCAGGCCACACAGACCGAAGTGGGCTGGGC AGTGTCCTGAGAGACCTAGTGAAGCCAGGCGATGAGAACCTTCGAGAAATGAACAAGAAGCTGCAGAACA TGTTGGAGGAGCAACTCACCAAGAACATGCACTTGCACAAGGACATGGAAGTTCTATCCCAGGAAATTGT GCGGCTCAGCAAGGAATGTGTGGGGTCCCCTGACCCAGACCTAGAACCTGGAGAAGCCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231233 representing NM_001290455 Red=Cloning site Green=Tags(s) MAQALSEEEFQRMQTQLLELRTNNYQLSDELRKNGVELSSLRQKVAYLDKEFSKAQKALSKSKKAQEVEV LLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQLEQLELENRQLKEGVPGAAGAHVDGELLRLQAEN TALQKNMAALQERYGKEAVRPSAVGEGQGDPPGDVLPTPLAPMPLAEVELKWEMEREEKKLLWEQLQGLE SSKQAETSRLQEELAKLSEKLKKKQESFCRLQTEKETLFNDSRNKIEELQQRKEADLKAQLARTQKLQQE LEAANQSLAELRDQRQGERLEHAAALRALQDQVSSQSADAQEQVEGLLAENSALRTSLAALEQIQTAKTQ ELNMLREQTSELASELQHRQAEYEELMGQKDDLNSQLQESLRANSRLLEQLQEIGQEKEQLTQDLQEARK SAEKRKVMLDELAMETLQEKSQHKEELGAVRLRHEKELLGVRARYERELRELHEDKKRQEEELRGQIREE KARTRELENLQHTVEELQAQVHSMDGAKGWFERRLKEAEESLQQQQQEQEETLKLCREEHAAELKGKDEE LQNVREQLQQAQEERDGHVKTISNLKQEVKDTVDGQRILEKKGSAVLKDLKRQLHLERKRADKLQERLQE ILTNSKSRTGLEELVLSEMNSPSRTQTGDSSSVSSFSYREILKEKESSAIPARSLSSSPQAQPPRPAELS DEEVAELFQRLAETQQEKWMLEEKVKHLEVSSASMAEDLCRKSAIIETYVMDSRIDVSVAAGHTDRSGLG SVLRDLVKPGDENLREMNKKLQNMLEEQLTKNMHLHKDMEVLSQEIVRLSKECVGSPDPDLEPGEAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290455
ORF Size	2511 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290455.1, NP_001277384.1
RefSeq Size	3021 bp
RefSeq ORF	2514 bp
Locus ID	54645
MW	96.4 kDa
Gene Summary	Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.