Snap91 (NM_001277983) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	91kDa; AP180; F1-20; mKIAA0656
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231234 representing NM_001277983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGCCAAACGCTCACGGATCGGATCGCCGCCGCTCAGTACAGCGTGACTGGCTCTGCTGTAGCAA GAGCAGTCTGCAAAGCCACCACTCATGAGGTGATGGGCCCCAAGAAGAAGCACCTGGACTATTTGATCCA GGCTACCAATGAGACCAATGTCAATATCCCTCAGATGGCCGACACCCTCTTTGAGCGGGCGACAAACAGT AGCTGGGTGGTGGTATTTAAAGCTTTAGTGACCACACACCATCTCATGGTGCATGGAAATGAGAGATTTA TTCAGTATTTGGCCTCTAGGAATACGCTATTCAATCTCAGCAACTTTCTAGATAAAAGTGGATCCCACGG TTATGATATGTCTACGTTCATACGGCGTTACAGTAGATACTTGAATGAAAAGGCTTTCTCCTACAGACAG ATGGCATTTGACTTTGCCAGAGTGAAGAAAGGGGCTGACGGTGTCATGAGGACGATGGTTCCTGAAAAGC TCCTGAAGAGTATGCCAATCCTGCAGGGGCAGATCGATGCACTGCTGGAGTTTGATGTGCATCCAAATGA ACTAACCAATGGTGTCATAAATGCTGCATTTATGCTTCTTTTCAAAGATCTTATCAAACTGTTTGCTTGC TACAATGACGGCGTCATTAACTTACTTGAAAAATTTTTCGAGATGAAGAAAGGTCAATGCAAAGACGCGC TAGAAATTTACAAGCGATTTCTAACTAGAATGACGAGGGTGTCTGAATTCCTCAAGGTCGCCGAGCAAGT TGGTATTGATAAAGGTGACATTCCCGACCTCACACAGGCTCCCAGCAGTCTTATGGAGACCCTTGAACAA CATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAAATCTGGTGCTCCCTCTCCACTAAGTAAGTCTT CTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAACTATCGACACATCCCCGCCAGTTGA CATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAGCCATCAAGCGATCTCCTTGATCTT CAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAGTGCCTCCTTCTGGGGGTGCGACCG CCTGGGGAGATTCCTTGGCTGCACTTTCCTCTGTTCCCTGTGAAGCACCGATTTCAGACCCATTTGCACC AGAGCCTTCCCCTCCTACTACAACCACTGAGCCTGCTTCAGCCTCTGCCTCGACCACCACAGCTGTGACG GCTGTCACTACGGAAGTGGATCTCTTTGGAGATGCCTTTGCAGCTTCTCCTGGGGAGGCCCCTGCAGCAT CCGAAGGGGCTACCGCACCAGCTACCCCGGCCCCAGTGGCTGCAGCTCTTGATGCATGCTCAGGAAATGA CCCTTTTGCCCCATCTGAAGGTAGCGCAGAGGCTGCACCTGAGCTGGACCTCTTTGCAATGAAGCCACCT GAGACCAGCGCTCCTGTAGTTACCCCTACAGCTAGCACAGCCCCTCCAGTTCCCGCAACTGCTCCTTCTC CTGCTCCCACGGCTGTGGCAGCCACTGCTGCCACCACCACCGCCGCCGCTGCAGCTACCACCACTGCCAC CACCTCTGCTGCTGCTGCCACCACCGCCGCTGCTCCTCCTGCTCTAGATATCTTTGGTGATTTGTTTGAT TCTGCTCCTGAAGTTGCTGCAGCACCTAAGCCAGACGCGGCTCCTAGCATAGACCTGTTTGGCACAGATG CTTTCTCCTCCCCGCCACGAGGGGCCTCTCCGGTGCCTGAGAGTTCTCTCACTGCTGACCTCTTATCTGT GGACGCATTTGCAGCGCCGTCTCCTGCATCCACTGCCTCTCCTGCAAAGGCGGAGTCCTCGGGTGTCATA GACCTTTTTGGGGGATTTGGGGGTTCTTTCATGGCCCCTTCTACAACGCCAGTGACTCCAGCTCAGAATA ACCTGCTGCAACCCAGTTTCGAGGCAGCTTTTGGAACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGA CCCATCAGTGTTTGATGGTTTAGGCGATCTTCTGATGCCAACCATGGCACCATCCGGGCAGCCTGCCCCT GTCTCAATGGTCCCACCCAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGGAAGTGACCTTGACTCGTCTC TGGCCAGTTTAGTAGGCAATCTTGGGATTTCTGGTACCACATCAAAAAAGGGAGATCTCCAGTGGAATGC TGGGGAGAAAAAGCTGACTGGTGGAGCCAACTGGCAACCGAAAGTCACTCCAGCCACATGGTCAGCGGGT GTTCCCCCGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCCAGTCATGTTTGCACAGCCCA TGATGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTTTCTCCAAGCCCTACACCTGCCAC TCAGAGTCCCAAGAAACCTCCAGCCAAGGACCCGTTAGCGGATCTTAACATCAAGGATTTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231234 representing NM_001277983 Red=Cloning site Green=Tags(s) MSGQTLTDRIAAAQYSVTGSAVARAVCKATTHEVMGPKKKHLDYLIQATNETNVNIPQMADTLFERATNS SWVVVFKALVTTHHLMVHGNERFIQYLASRNTLFNLSNFLDKSGSHGYDMSTFIRRYSRYLNEKAFSYRQ MAFDFARVKKGADGVMRTMVPEKLLKSMPILQGQIDALLEFDVHPNELTNGVINAAFMLLFKDLIKLFAC YNDGVINLLEKFFEMKKGQCKDALEIYKRFLTRMTRVSEFLKVAEQVGIDKGDIPDLTQAPSSLMETLEQ HLNTLEGKKPGNKSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDIFATASAAAPVSSAKPSSDLLDL QPDFSGAAAGAAAPVVPPSGGATAWGDSLAALSSVPCEAPISDPFAPEPSPPTTTTEPASASASTTTAVT AVTTEVDLFGDAFAASPGEAPAASEGATAPATPAPVAAALDACSGNDPFAPSEGSAEAAPELDLFAMKPP ETSAPVVTPTASTAPPVPATAPSPAPTAVAATAATTTAAAAATTTATTSAAAATTAAAPPALDIFGDLFD SAPEVAAAPKPDAAPSIDLFGTDAFSSPPRGASPVPESSLTADLLSVDAFAAPSPASTASPAKAESSGVI DLFGGFGGSFMAPSTTPVTPAQNNLLQPSFEAAFGTTPSTSSSSSFDPSVFDGLGDLLMPTMAPSGQPAP VSMVPPSPAMAASKGLGSDLDSSLASLVGNLGISGTTSKKGDLQWNAGEKKLTGGANWQPKVTPATWSAG VPPPPSGTGMTMMSQQPVMFAQPMMRPPFGAAAVPGTQLSPSPTPATQSPKKPPAKDPLADLNIKDFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277983
ORF Size	2514 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277983.1, NP_001264912.1
RefSeq Size	4179 bp
RefSeq ORF	2517 bp
Locus ID	20616
MW	86.5 kDa
Gene Summary	Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.[UniProtKB/Swiss-Prot Function]

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