C7 (NM_001243837) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A530026G17; Gm1238
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231245 representing NM_001243837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTGACAAGCTTATTAATTTTGGTGTGCTTTATAGCAGCGTTTCAAGTCTTCTCTAGAGCCTCCT CTCCAGTCAACTGCAAGTGGGACTCCTATGGCCCTTGGTCAGAATGCAATGGCTGTACCAAGACTCAGAC TCGAAGGCGTTCAGTTGCTGTTTATGGGCAATATGGAGGCTATCCATGTGAGGGGAGTGCTTTTGAAACA CAATCCTGCAAACCTGAACGAGGCTGTCCGACGGAAGAGGGCTGTGGGGACCGGTTTAGGTGTTTTTCAG GTCAGTGCATCAGCAAATCATTGGTTTGCAATGGAGATCCTGACTGTGAGGAAGACGGTGCTGATGAAGA CAAATGTGAAAATGTGGCTAACCCTTCCTGCAACATTGATAAGCCTCCTCCCAACATAGAACTTACCGGT TATGGTTACAACGTAGTTACTGGCCAGGGTAAGAAAAGAGTCATCAACACCAAAAGTTTCGGTGGTCAGT GCAGGAAAGTGTTCAGCGGGGATGGAAAAGATTTCTACAGACTAAGTGGAAATATCCTCTCTTATACATT CCAGGTCAAAGTAGATAATGATTTTAACTATGAATTTTACAACAGCAGTTGGTCTTACATAAAGCACACA TCCACAGAGCAAAACACATTTTATTCATGGAAGGGATTATTTTCCCACTCACGGAATACTTACGGTCATG GTTCAGCAAAAGAGGAAATCGACACGAAAATGAAGAGTTATAAATTGTTGGTTGTTCAGAACACTGTTGA AGTGGCCCAGTTCACTAATAATAATCCTGAATTTTTACAAGTTGCTGAGCCATTCTGGAAGGAACTCTCC CATCTTCCCACTCTGTATGACTACAGTGCCTACCGACGGTTAATCGATCAGTATGGGACACATTACCTGC AGTCTGGGTCCTTAGGAGGAGAATATAGAGTTCTATTTTATGTGGATTCAGGAAGTGCCAAAGAAACTGG TTTTCAGTCAGACCAGGATAACGCATGTTCGTCTGCAGACTTTCAGTTTCTTTTTACATCGTCAGCAGAT CAGAGATGTATGAAACAGCTAGAGACTGAAAAATCAACTTCAGGAAATAAGGGCAGGTTGCTTCGAGGAA AACCATTGGTCAGAGGTGGAGACTCTGGCTTTGTAGCTGACCTTAGTTTCCTGGACCTGGATAATCCTGC TGGAAACAAGCAGCGCTATTCCTCCTGGGCTGGGTCTGTAACCAGACTTCCCCAAGTCATAAAAGAAAAG CTGGCGCCGTTGTATGAGCTGGTCAAGGAGGTGCCCTGTGCCTCTGTGAAAAGACTGTACCTGAAGCGGG CTATTGAGGAGTACTTCGATGAATTTGACCCCTGCCATTGCCGGCCTTGCCAGAATGGCGGCCTCGCTAT TGTTGTGGAGACACAGTGCCAGTGTCTGTGCAAACCATACACATTTGGTTCAGCGTGTGAGCAAGGAGTC CTTGTCGGCGATCAAGCAGGAGGGGTTGATGGAGGTTGGAATTGTTGGTCCTCTTGGAGCCCCTGTGTTC AAGGGAAGAGAACAAGGAGCCGGGAATGCAATAATCCCCCTCCCAGAGATGATGGAAAGTCCTGTCTTGG AGAAACAACTGAGAGTAAGCAGTGTGAAGACCAGGACTTGGAGAAACTGCGATTACTTGAGCCACATTGC TTTCACTCGTCTTTGGCTCCAAAAGAATTCTGCCTATCACCCCCTGCCTTAAAAGATGGATTTGTTCAAG GTGAAGGTACCATGGTTCCTGTTGGACAAAGCGTAGTATATGCTTGTGATGAGGGGTATTCTCTTATTGG AGATCCTGTTGCCAGATGTGGAGAAGACTTACAGTGGCTTGTTGGAGAAATGCATTGTCAAAAATTGGCC TGCGTTCTACCTGGAGAGATGAATGGCATGCAGAGTCACCCGCAGAAACCTTTTTACATGGTTGGAGAGA AGGTGACTCTTTCCTGTGTGGGGGGTATGTCCTTAGAGGGTCCTTCAACCCTCCTCTGTGGCTCCAGCCT GAAGTGGAGTCCCGAGCTGAAGGATGCCAAGTGTGTGCAAAAAGCTACCAGCACTCCACCACCAGCAGCC CCTGCATGTCAGCCTTGGGAGAAACTGCAGAACTCAAAATGCATCTGTAAAATGCCCTATGAATGTGGAT CCTCCTTGGATGTATGTGCCCGAGATGAGAGTAGAAACAAGATTCTGTCTCTGACCATCTGCAAGATGCA CGTTGTCCACTGTCATAAAAGAAATTACACTCTTGTTGGCAAGGAAAGCTGTACTCTGCCTTCCCCGGCT GAGAAAGTCTGTGGTGCATGCCCAATATGGAGTAAATGTGATGCTCAGAATGGCAAATGTATCTGCAGAG AAGCATCGGAGTGCCAGACAGCAGGCTACAGAATCTGTGTGGAAGTGAATGGCAAAGAGGAAACGATGTC TGAGTGTGAGGCTGGCATCTTAAGATGCAGAGGACAGAGCATCTCCATCACAGCCATAAAGCCCTGTGCA GAAGAAGCAGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231245 representing NM_001243837 Red=Cloning site Green=Tags(s) MQVTSLLILVCFIAAFQVFSRASSPVNCKWDSYGPWSECNGCTKTQTRRRSVAVYGQYGGYPCEGSAFET QSCKPERGCPTEEGCGDRFRCFSGQCISKSLVCNGDPDCEEDGADEDKCENVANPSCNIDKPPPNIELTG YGYNVVTGQGKKRVINTKSFGGQCRKVFSGDGKDFYRLSGNILSYTFQVKVDNDFNYEFYNSSWSYIKHT STEQNTFYSWKGLFSHSRNTYGHGSAKEEIDTKMKSYKLLVVQNTVEVAQFTNNNPEFLQVAEPFWKELS HLPTLYDYSAYRRLIDQYGTHYLQSGSLGGEYRVLFYVDSGSAKETGFQSDQDNACSSADFQFLFTSSAD QRCMKQLETEKSTSGNKGRLLRGKPLVRGGDSGFVADLSFLDLDNPAGNKQRYSSWAGSVTRLPQVIKEK LAPLYELVKEVPCASVKRLYLKRAIEEYFDEFDPCHCRPCQNGGLAIVVETQCQCLCKPYTFGSACEQGV LVGDQAGGVDGGWNCWSSWSPCVQGKRTRSRECNNPPPRDDGKSCLGETTESKQCEDQDLEKLRLLEPHC FHSSLAPKEFCLSPPALKDGFVQGEGTMVPVGQSVVYACDEGYSLIGDPVARCGEDLQWLVGEMHCQKLA CVLPGEMNGMQSHPQKPFYMVGEKVTLSCVGGMSLEGPSTLLCGSSLKWSPELKDAKCVQKATSTPPPAA PACQPWEKLQNSKCICKMPYECGSSLDVCARDESRNKILSLTICKMHVVHCHKRNYTLVGKESCTLPSPA EKVCGACPIWSKCDAQNGKCICREASECQTAGYRICVEVNGKEETMSECEAGILRCRGQSISITAIKPCA EEAAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243837
ORF Size	2535 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243837.1, NP_001230766.1
RefSeq Size	2709 bp
RefSeq ORF	2538 bp
Locus ID	109828
MW	93.8 kDa

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