Map4k3 (NM_001290345) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4833416M01Rik; 4833416M07Rik; 9530052P13Rik; Glk; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231341 representing NM_001290345 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCCGGGCTTCGATCTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATCCAGCGCATCGGCA GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTCAACACTGGAGAATTAGCAGCAATTAAAGTGAT AAAATTGGAACCAGGAGAAGACTTTGCGGTTGTACAGCAAGAAATCATTATGATGAAAGACTGCAAACAC CCAAACATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAACTTTGGATTTGCATGGAATTCTGTG GAGGTGGCTCTTTACAGGACATATATCATGTAACTGGACCCCTTTCAGAACTGCAGATTGCATATGTTAG CAGAGAAACTCTACAGGGATTGTATTACCTTCACAGTAAAGGAAAAATGCACCGAGATATAAAGGGCGCT AACATTCTCCTAACGGATAATGGTCACGTGAAACTAGCTGATTTTGGAGTTTCTGCACAGATAACAGCTA CAATTGCCAAACGGAAGTCCTTCATTGGAACGCCATATTGGATGGCCCCAGAAGTCGCGGCTGTTGAGAG GAAAGGCGGGTACAATCAACTGTGTGACCTCTGGGCGGTGGGCATCACTGCCATCGAGCTGGCGGAGCTC CAGCCACCCATGTTTGACCTTCACCCGATGAGGGCATTGTTTCTAATGACAAAAAGCAATTTCCAGCCTC CTAAACTAAAAGATAAGTTGAAGTGGTCAAATAGTTTCCATCATTTTGTGAAGATGGCTCTTACCAAAAA TCCAAAGAAAAGACCCAATGCAGAAAAGCTGTTACAGCATCCCTTTGTCACACAGCCTCTGACACGGTCT TTGGCAATCGAGTTGCTGGACAAAGTGAATAATCCAGACCATTCCACATACCACGATTTTGATGATGATG ACCCAGAGCCTCTTGTTGCCGTACCACATAGAATTCCTTCAACGAGTAGAAATGTGAGAGAAGAAAAAAC ACGCTCAGAGATAAACTTTGGCCAGGTAAAATTTGATCCGCCCTTAAGGAAGGAGACAGAGCCACATCAC GAACTTCCCGACAGTGATGGTTTTTTCGACAGTTCAGAAGAAATATACTACACTGCAAGATCTAATCTGG ATCTGCAACTAGAGTACGGACAAGGACACCAAAGCCATTGCTTCTTAGGTGGCAACAAGAGTCTTCTGAA GTCTGTTGAAGAGGAATTACATCAGCGAGGACATGTGGCACACTTAGAAGATGACGAAGGCGACGATGAC GACTCTAAGCATTCAACCATGAAAGCAAAAGTCCCGCCTCCTCTGCCACCAAAGCCTAAATCCATCTTCA TACCACAGGATACACATTCTGCTGAGGACGGTAATCAAGGAACAATCAAGAGATGTCCCTCATCAGGGAG CCCGGCAAAGCCATCCCACGTTCCACCTAGACCACCTCCGCCCAGGTTACCGCCACAGAAGCCTGCTGTT TTAGGAAATGGAGTGAACTCCTTCCAGCTAAATGGTGAGCGAGACGGATCATTATATCAGCAGCAGAGTG AGCAAAGAGGCACAAACCTTTCAAGAAAGGAGAAGAAGGACGTCCCGAAACCAATTAGTAATGGTCTTCC CCCAACACCTAAAGTGCATATGGGAGCGTGTTTTTCAAAGGTTTTTAATGGATGTCCCTTGAAAATCCAC TGTGCCACATCCTGGATAAACCCAGACACGAGAGACCAGTACTTGATATTTGGCGCTGAAGAGGGCATTT ATACCCTTAATCTCAATGAACTTCATGAAACATCAATGGAACAGCTGTTCCCCCGCAGGTGTACGTGGCT GTATGTGATGAACAATTGCCTGCTGTCAGTCTCGGGCAAAGCCTCTCAGCTTTACTCCCACAACTTACCG GGGCTTTTCGATTATGCAAGACAGATGCAGAAGCTTCCAGTAGCCATCCCAGCACACAAGCTCCCCGACC GAATACTGCCCAGGAAATTTGCTGTATCAGCAAAGATTCCTGAAACCAAATGGTGCCAGAAGTGCTGCGT GGTGAGAAATCCTTACACGGGCCATAAGTACCTGTGCGGGGCGCTTCAGACCAGCATTGTTCTATTAGAA TGGGTTGAGCCAATGCAGAAATTTATGTTAATTAAGCACATAGAGTTTCCTATGCCGTGTCCACTCCGGA TGTTTGAGATGCTGGTGGTCCCTGAGCAGGAGTACCCTCTGGTCTGTGTTGGCGTCAGCAGAGGGCGAGA CTTCAACCAAGTGGTTCGGTTTGAGACGGTCAATCCCAATTCTACCTCGTCGTGGTTTACAGAGTCAGAT GCCCCGCAGACAAGCGTCACTCATGTGACCCAGCTGGAGAGAGACACCATCCTTGTGTGCTTGGATTGTT GTATAAAAATAGTCAATCTCCAAGGAAGGTTGAAATCCAGCAGGAAATTGTCATCAGAACTCACCTTCGA TTTCCAGATAGAGTCCATAGTTTGTCTACAAGACAGTGTGCTAGCGTTCTGGAAACACGGGATGCAAGGT AGAAGCTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAACACAAGAATCTTCAGACTCCTTGGAT CTGACAGGGTTGTGGTTTTGGAAAGTCGGCCAACTGATAACCCAACAGCAAATAGCAATTTATACATCTT GGCGGGTCACGAAAACAGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231341 representing NM_001290345 Red=Cloning site Green=Tags(s) MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKH PNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGA NILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAEL QPPMFDLHPMRALFLMTKSNFQPPKLKDKLKWSNSFHHFVKMALTKNPKKRPNAEKLLQHPFVTQPLTRS LAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIPSTSRNVREEKTRSEINFGQVKFDPPLRKETEPHH ELPDSDGFFDSSEEIYYTARSNLDLQLEYGQGHQSHCFLGGNKSLLKSVEEELHQRGHVAHLEDDEGDDD DSKHSTMKAKVPPPLPPKPKSIFIPQDTHSAEDGNQGTIKRCPSSGSPAKPSHVPPRPPPPRLPPQKPAV LGNGVNSFQLNGERDGSLYQQQSEQRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIH CATSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSVSGKASQLYSHNLP GLFDYARQMQKLPVAIPAHKLPDRILPRKFAVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLE WVEPMQKFMLIKHIEFPMPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESD APQTSVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQG RSFRSNEVTQEISDNTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290345
ORF Size	2682 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290345.1, NP_001277274.1
RefSeq Size	4176 bp
RefSeq ORF	2685 bp
Locus ID	225028
UniProt ID	Q99JP0
MW	101.6 kDa
Gene Summary	May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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