Atp2c1 (NM_001253831) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700121J11Rik; ATP2C1A; AW061228; BCPM; D930003G21Rik; HHD; pmr1; SPCA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231420 representing NM_001253831 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACTTATTGCCTCCGTCCGGATTTTCTTACTTGAAAAAGTATCCTCTCCACTCTATTCGGAAAT ATTTATCAACGCTGAGAAGCCAAAGGGCCGAAGAGCAGGTTGCACGATTTCAAAAGATCCCTAATGTTGA AAATGAGACAATGATCCCTGTACTGACGTCAAAGAGAGCAAGTGAGTTAGCAGTCAGTGAGGTTGCAGGC CTTCTCCAGGCTGATCTTCAGAATGGCTTAAACAAATCTGAAGTTAGTCATAGGCGAGCCTTCCATGGTT GGAATGAATTTGATATCAGTGAAGATGAACCATTATGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCT TATCATGCTGCTCCTGGCGTCTGCGGTCATCAGCATTTTAATGCGCCAGTTTGATGATGCCGTCAGTATC ACTGTGGCAATAGTAATTGTCGTCACTGTGGCCTTTGTTCAGGAATATCGTTCAGAAAAATCTCTAGAAG AATTGAGTAAACTTGTGCCACCAGAATGCCATTGTGTGCGTGAAGGAAAACTGGAGCATACACTTGCCCG AGACTTGGTTCCAGGTGACACAGTTTGTCTCTCTGTGGGGGACAGAGTTCCTGCGGACTTACGCTTATTT GAGGCTGTGGATCTTTCTGTGGATGAGTCTAGCTTGACAGGAGAGACAGCTCCGTGCTCTAAAGTGACAG CCCCTCAGCCAGCTGCTAATGGAGATCTTGCGTCAAGAAGTAACATCGCCTTCATGGGGACACTGGTCAG ATGTGGCAAAGCAAAGGGTATTGTCATTGGAACAGGAGAAAATTCTGAATTTGGAGAGGTCTTTAAGATG ATGCAGGCAGAAGAAGCACCAAAAACTCCTCTGCAGAAGAGCATGGACCTCTTAGGCAAGCAGCTGTCCT TTTACTCCTTTGGTATAATAGGTATCATCATGTTGGTTGGCTGGTTACTAGGAAAAGACATTCTGGAAAT GTTCACTATTAGTGTAAGCTTGGCTGTAGCTGCAATTCCTGAAGGCCTGCCTATTGTGGTCACGGTGACT CTAGCCCTTGGTGTTATGAGAATGGTGAAGAAAAGGGCTATTGTAAAGAAATTGCCTATTGTGGAAACAC TGGGCTGCTGTAATGTGATTTGTTCAGATAAAACTGGAACCCTGACGAAGAACGAGATGACTGTTACTCA CATCCTCACTTCCGATGGCCTGCATGCTGAGGTCACTGGAGTTGGCTATAATCAGTTTGGTGAAGTGATC GTTGATGGTGATGTTGTTCATGGATTTTATAACCCAGCTGTTAGCAGAATTGTTGAGGCAGGCTGTGTGT GCAATGATGCTGTAATTAGGAACAACACTCTGATGGGAAAGCCAACTGAAGGAGCCTTAATCGCTCTTGC TATGAAGATGGGTCTTGATGGACTGCAACAAGACTACATCAGGAAAGCTGAATACCCTTTTAGTTCTGAG CAGAAGTGGATGGCTGTTAAGTGTGTGCACCGAACACAGCAGGACAGACCAGAGATTTGTTTTATGAAGG GTGCTTATGAGCAGGTGATTAAGTATTGTACTACATACAACAGCAAAGGGCAGACTTTGGCACTTACCCA GCAGCAGAGAGATTTGTATCAACAAGAGAAGGCACGGATGGGCTCAGCGGGACTCAGAGTTCTTGCCTTG GCGTCTGGTCCGGAACTGGGGCAGCTGACCTTCCTTGGCCTGGTCGGAATCATTGACCCTCCTAGAACTG GTGTGAAGGAAGCTGTCACAACACTCATTGCCTCAGGAGTCTCCATCAAAATGATCACTGGAGATTCTCA GGAGACTGCAATTGCCATCGCTAGTCGTCTGGGATTGTACTCTAAGACTTCACAGTCCGTGTCTGGGGAA GAAGTCGATACAATGGAGGTGCAGCACCTTTCACAGATAGTGCCAAAGGTTGCAGTATTTTACAGAGCAA GCCCAAGACACAAGATGAAAATTATTAAGTCTCTACAGAAGAACGGGGCAGTTGTAGCCATGACAGGAGA TGGGGTAAATGATGCAGTTGCTCTCAAGGCTGCAGACATTGGAGTTGCGATGGGCCAGACTGGCACCGAT GTTTGCAAAGAGGCTGCAGACATGATCTTGGTGGATGATGATTTCCAAACCATCATGTCTGCAATAGAAG AGGGTAAAGGCATTTATAATAACATTAAAAATTTTGTTAGATTTCAACTGAGCACGAGTATAGCAGCATT AACTTTAATCTCATTGGCTACGTTAATGAACTTTCCTAACCCTCTCAATGCAATGCAGATTTTGTGGATC AATATTATAATGGATGGACCCCCAGCTCAGAGCCTTGGAGTAGAGCCAGTGGATAAAGATGTCATTCGAA AACCGCCTCGAAACTGGAAGGACAGCATTTTGACAAAAAACTTGATACTTAAAATACTTGTTTCATCAAT AATCATTGTTTGTGGGACTTTGTTTGTCTTCTGGCGAGAGCTTCGAGACAATGTGATAACACCCCGAGAC ACAACCATGACTTTCACTTGCTTTGTGTTTTTTGACATGTTCAATGCACTGAGTTCCAGATCTCAGACCA AGTCTGTGTTTGAGATTGGACTCTGCAGTAATAAGATGTTCTGCTATGCAGTTCTTGGATCCATCATGGG ACAGTTGCTTGTCATTTACTTCCCTCCTCTCCAGAAGGTTTTTCAAACCGAGAGCCTGAGTATACTGGAT CTGTTGTTTCTTTTGGGCCTTACCTCGTCAGTGTGCATTGTGTCTGAGATTATAAAGAAGGTTGAAAGGA GCCGGGAGAAGGTCCAGAAGAATGCTGGTTCAGCATCATCGTCTTTCCTTGAAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231420 representing NM_001253831 Red=Cloning site Green=Tags(s) MENLLPPSGFSYLKKYPLHSIRKYLSTLRSQRAEEQVARFQKIPNVENETMIPVLTSKRASELAVSEVAG LLQADLQNGLNKSEVSHRRAFHGWNEFDISEDEPLWKKYISQFKNPLIMLLLASAVISILMRQFDDAVSI TVAIVIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLF EAVDLSVDESSLTGETAPCSKVTAPQPAANGDLASRSNIAFMGTLVRCGKAKGIVIGTGENSEFGEVFKM MQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTVT LALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHILTSDGLHAEVTGVGYNQFGEVI VDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSE QKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYNSKGQTLALTQQQRDLYQQEKARMGSAGLRVLAL ASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETAIAIASRLGLYSKTSQSVSGE EVDTMEVQHLSQIVPKVAVFYRASPRHKMKIIKSLQKNGAVVAMTGDGVNDAVALKAADIGVAMGQTGTD VCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWI NIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNLILKILVSSIIIVCGTLFVFWRELRDNVITPRD TTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNKMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSILD LLFLLGLTSSVCIVSEIIKKVERSREKVQKNAGSASSSFLEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253831
ORF Size	2856 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253831.1, NP_001240760.1
RefSeq Size	4683 bp
RefSeq ORF	2859 bp
Locus ID	235574
UniProt ID	Q80XR2
MW	104.8 kDa
Gene Summary	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.