Mllt10 (NM_001252561) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Af10; B130021D15Rik; D630001B22Rik; mKIAA4140
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231465 representing NM_001252561 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGATGTGAGCTGTGTCCCCATAAAGATGGAGCTTTAAAAAGAACAGATAATGGGGGTTGGGCTC ATGTGGTTTGTGCCCTCTATATTCCAGAGGTACAATTTGCCAATGTTTCTACAATGGAGCCAATCGTTTT ACAGTCTGTCCCACATGATCGTTATAATAAGACTTGCTATATATGTGATGAACAAGGACGAGAAAGCAAA GCAGCCACTGGTGCTTGCATGACATGTAATAAACATGGATGTCGACAGGCTTTCCATGTAACATGTGCTC AGTTTGCCGGGCTGCTTTGTGAAGAAGAAGGGAATGGCGCAGACAATGTTCAATACTGTGGCTACTGTAA ATACCACTTTAGTAAGCTGAAAAAGAGCAAACGGGGATCTAATAGGTCATATGAGCAAAGTTTAAGTGAC TCTTCCTCTCACTCTCAGGATAAGCATCATGAGAAAGAGAAAAAAAAATATAAAGAAAAGGACAAACACA AACAAAAACACAAGAAGCAACCAGAACCGTCACCTGCCTTGGTTCCTTCTTTGACTGTTACTACAGAAAA AACTTATACAAGCACTAGCAACAACTCCATATCTGGATCATTGAAGCGCTTGGAAGATACTGCAGCAAGA TTTACAAATGCAAATTTCCAGGAAGTCTCTGCCCATACCTCTAGTGGAAAAGATGTTTCAGAGGCTAGAG GGTCAGAGGGCAAAGGGAAGAAATCTTCAGCTCACAGCTCAGGTCAAAGGGGAAGGAAGCCTGGTGCAGG AAGAAATCCAGGAACAGCTGTGTCAGCATCTAGCCCCTTTCCACAAGGCAGTTTTTCAGGAACTCCAGGC AGTGTAAAGTCATCTTCTGGAAGTTCAGTGCAGTCTCCCCAGGACTTCTTAAGCTTTACAGACTCAGATC TGCGAAGTGACAGTTACACTCACACCCAGCAGCCATCATCAACCAAAGATGTACATAAAGGAGAGTCTGG AAGCCAGGAAGCGGCAGTAAACAGTTTTAGCTCTTTAGTTGGTCACCCTGTGACCTCGACTGTTATTTCA CAGCCTAAAAGCTTTGACAATTCACCTGGAGAGTTGGGTAGTTCGAGCCTCCCTACAGCAGGATATAAGC GGGCTCAGACTTCTGGTATAGAAGAAGAAGCTGTAAAGGAAAAGAAACGAAAAGGAAATAAGCAAAGCAA ACATGGCCCCGGCCGACCCAAAGGAAACAAAAATCAAGAAAATGTTTCTCACCTCTCAGTTTCTTCTGCT TCACCAACATCGTCCGTAGCATCAGCTGCAGGAAGTGTAACAAGCTCTAGTCTTCAGAAATCTCCTACGC TGCTCAGGAATGGAAGTTTGCAGAGTCTTAGTGTTGGCTCGTCTCCAGTTGGCTCAGAAATTTCCATGCA GTATCGACATGATGGAGCTTGTCCAACAACTACCTTCTCAGAGTTGCTGAATGCAATACACAATGGTATT TATAACAGCAATGATGTAGCCGTATCCTTTCCAAACGTGGTGTCTGGCTCAGGATCTAGTACTCCTGTCT CTAGCTCTCATATACCTCAGCAGTCATCTGGTCATTTGCAACAGGTGGGAGCTCTCTCCCCCTCAGCTGC GTCATCTGTAACCCCTGCTGCTGCTACAACTCAGGCAAATACCGTTTCTGGATCTTCTCTCAGTCAGGCA CCAGCCCATATGTATGGCAGTAGATTAAATCAGAATCCATCCATGGCAGTTCTCATAGCTCAGTCTGAAA GCAGCCAAACAGATCAAGATCTTGGAGACAATGCCCGTAGCCTGGGTGGCCGAGGAAGCTCGCCCCGAGG AAGCCTCTCTCCGAGATCCCCTGTGAGCAACTTACAGCTTCGCTATGATCAACCGAGCAATAGCAGTTTG GAAACTGTGCCTCCAGTAGCAGCAAGTATTGAACAGCTCTTGGAGAGGCAGTGGAGTGAAGGACAGCAGT TTTTACTAGAGCAGGGGACTCCTGGTGACATTCTAGGAATGTTAAAGTCATTACATCAACTTCAAGTTGA AAATCGAAGATTAGAAGAACAGATCAAAAATTTGACTGCCAAGAAAGAACGTCTTCAGTTGTTGAATGCA CAGCTTTCAGTGCCTTTCCCAGCCATAACGACAAACCCCAGTCCGTCCCATCAGATGCACACATACACAG CACAGACTGCTCCTCCTCCTGATTCCCTGAACAGCAGTAAGAGCCCACATATAGGAAACAGCTTTTTACC TGACAATTCTCTTCCTGTATTAAATCAGGACTTGACCTCCAGTGGACAAAGCACCAGCAGTTCTTCTGCT CTTTCTACTCCCCCTCCTGCTGGCCAGAGTCCTGCTCAGCAGAGCTCTGGAGTCAGTGGGGTTCAGCAGG TGAATGGCGTGACAGTGGGGGCACTAGCTAGTGGGATGCAGACCGTCACATCCACCATCCCAGCTGTGTC CGCAGTGGGTGGAATAATTGGAGCTCTGCCAGGTAACCAACTGGCAATTAACGGCATTGTGGGAGCTTTA AATGGTGTGATTCAGACTCCAGTCACAATCTCCCAGAACCCTGCCCCTCTCACCCACACCAGTGTACCAC CTAATGCAGCGCATCCAATGCCGGCCGCCGCACTGACCAACAGTGCCTCGGGACTAGGGCTGCTGTCGGA CCAGCAGAGACAGATGTTTATCCAGCAGCAGCAGTTCCAGCAGCTGTTAAACTCCCAGCAGCTCACACCC GAACAGCATCAGGCCTTCCTATACCAGCTGATGCAGCAGCAGCACCACCCGCCTGAGCTCCAGCAGCTGC AGCTCCCGGGACCCACGCAGATCCCCATCAACAATCTCCTTGCAGGTGCTCAGGCGCCTCCACTCCACAC AGCTACCACCAACCCCTTCCTCACCATCCACGGGGACAGCACGAGCCAGAAGGTCACAAGACTTAGTGAT AAAACTGGGCCTGTAGCTCAAGAGAAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231465 representing NM_001252561 Red=Cloning site Green=Tags(s) MKRCELCPHKDGALKRTDNGGWAHVVCALYIPEVQFANVSTMEPIVLQSVPHDRYNKTCYICDEQGRESK AATGACMTCNKHGCRQAFHVTCAQFAGLLCEEEGNGADNVQYCGYCKYHFSKLKKSKRGSNRSYEQSLSD SSSHSQDKHHEKEKKKYKEKDKHKQKHKKQPEPSPALVPSLTVTTEKTYTSTSNNSISGSLKRLEDTAAR FTNANFQEVSAHTSSGKDVSEARGSEGKGKKSSAHSSGQRGRKPGAGRNPGTAVSASSPFPQGSFSGTPG SVKSSSGSSVQSPQDFLSFTDSDLRSDSYTHTQQPSSTKDVHKGESGSQEAAVNSFSSLVGHPVTSTVIS QPKSFDNSPGELGSSSLPTAGYKRAQTSGIEEEAVKEKKRKGNKQSKHGPGRPKGNKNQENVSHLSVSSA SPTSSVASAAGSVTSSSLQKSPTLLRNGSLQSLSVGSSPVGSEISMQYRHDGACPTTTFSELLNAIHNGI YNSNDVAVSFPNVVSGSGSSTPVSSSHIPQQSSGHLQQVGALSPSAASSVTPAAATTQANTVSGSSLSQA PAHMYGSRLNQNPSMAVLIAQSESSQTDQDLGDNARSLGGRGSSPRGSLSPRSPVSNLQLRYDQPSNSSL ETVPPVAASIEQLLERQWSEGQQFLLEQGTPGDILGMLKSLHQLQVENRRLEEQIKNLTAKKERLQLLNA QLSVPFPAITTNPSPSHQMHTYTAQTAPPPDSLNSSKSPHIGNSFLPDNSLPVLNQDLTSSGQSTSSSSA LSTPPPAGQSPAQQSSGVSGVQQVNGVTVGALASGMQTVTSTIPAVSAVGGIIGALPGNQLAINGIVGAL NGVIQTPVTISQNPAPLTHTSVPPNAAHPMPAAALTNSASGLGLLSDQQRQMFIQQQQFQQLLNSQQLTP EQHQAFLYQLMQQQHHPPELQQLQLPGPTQIPINNLLAGAQAPPLHTATTNPFLTIHGDSTSQKVTRLSD KTGPVAQEKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252561
ORF Size	2970 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252561.1, NP_001239490.1
RefSeq Size	5116 bp
RefSeq ORF	2973 bp
Locus ID	17354
MW	105 kDa
Gene Summary	Probably involved in transcriptional regulation. Binds to cruciform DNA (By similarity). In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (By similarity).[UniProtKB/Swiss-Prot Function]

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