Palld (NM_001293773) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2410003B16Rik; 6030492A02
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231473 representing NM_001293773 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACAAGCTCAGAAGAAAACAACGTCCGTTTCCTTAACGATAGGATCCTCAGCTCCGAAAACAGGAG TGACCACAGCTGTGATTCAGCCCCTATCTGTGCCCGTCCAACAGGCTCACAGCGCTACTTCATATCTCTG CCGACCTGACGGAACCACCATGGGCTGCCTTCTTCCTGTTTTTACTAAGGAACTACAGAACACAGCAGCC TCCGAGGGCCAGGTGGTGGTGCTGGAATGCAGAGTCCGAGGGGCACCCCCGCTGCAGGTCCAGTGGTTCC GGCAAGGAAGTGAGATCCAAGACTCTCCGGACTTCAGAATTCTCCAGAAAAAACCCAGATCGACAGCTGA ACCCGAGGAGATCTGCACCCTTGTCATTGCTGAGAGTTTCCCTGAAGATGCAGGCATCTTTACCTGCTCA GCCACAAATGACTACGGCTCAGTGACCAGCACTGCACAGCTTGTTATAACCTCCGCCAACAACGAGAACT GTAGTTACGACTCAACAGGAGAACCCAACAGTGATCACTTCCAACACTTTCCACCTCCCCCTCCGATCCT GGAGACAGGCTCCTATGAGCTGGCATCCCAGAAACCATCTGAAATCCAGCAGGTGAACAGCCCCAATTTA GGATTTAGCATGGCAGCTCTTCAAATGCAATTCAACACTGCAGAGAGGGAGACCAACGGAGTCCATCCCA GCCATGGAGTCAACGGGCTGATTAATGGCAAAGCTTATGGCAATAAATCTCCTCCAACACCAACTGCCCT GCTTTCACCCACTAAGGAACCACCGCCACTCCTTGCCAAACCCAAACTGGATCCTCTGAAGCTTCAGCAG CTGCAGAACCAAGTGCGCCTGGAACAGGAGGCCTGCGCCTGGCCCCCAGCGCCCCCAGGTGTCCCTTGCA ACAGCAGCAGCAGCGGCAGCAGCGCGCCCCCGTCGCCGCCCTTCCCCCCACCGCCACCAGCCTTTCCGGA GCTCGCGGCCTGTGCGTCGCCGGTGCCCTCGGAGCCCATGAGCGCTCTGGCCTCCCGCGCTACCGCCATG CAGTCCTCCGGCTCCTTCAACTACGCGCGCCCTAAGCAGTTCATCGCGGCGCAGAACCTGGGTCCCGCAT CGGGGCTGCCCACGCCAACCTCCAGCCCCAGCTCCTCCAGCTTGCCGTCACCATTGTCCCCCACGCCCAG GCCCTTCGGCCGCGCGCCCGGGCCGCCCTTCGTGGAACCCGAGGCCATGTGGGGCCCGTCCTCGCCCTCG CCGCCACCGCCGCCACCTCCGGTCTTCAGCCCCTCTGCAGCCTACCCGGTGCCCGACGTGTTCCCACTGC CACCGCCGCCGCCACCGCTACCTAGCTCCACCTCACACTGCGCCTCGCCCGCCCGCTTCGGCCCCAGCCA GACGCCCGCAGCCTTCCTCAGCGCCCTGCTGCCCTCTCAGCCACCGCCTGTCGCTGTCAACGCCCTGGGG CTGCCCAAGGGAGTCACCCCCGCGGGGTTCCCAAAGAAGTCCAGTAGAACTGCTAGAATTGCCTCTGATG AGGAGATTCAAGGCACAAAGGATGCTGTCATCCAAGACCTGGAACGGAAGCTTCGCTTCAAGGAGGACCT TCTGAACAATGGCCAACCGAGGCTAACCTATGAGGAAAGAATGGCTCGCCGCCTGCTTGGAGCCGACAGC GCAAACGTCTTCAACATCCAGGAGCCAGAGGAAACGGCAGCCAATCAGGAGTACAAAGTCTCTAGCTGCG AGCAGAGGCTGATTAGCGAGATTGAGTACCGGCTGGAGCGCTCCCCTGTGGATGAGTCGGGAGACGAGGT GCAGGATCCAGATGTGCCTGTGGAGAACGCAACAGCTCCCTTCTTTGAGATGAAGCTGAAACACTACAAG ATCTTTGAGGGGATGCCGGTGACTTTCACGTGTCGAGTGGCTGGGAATCCAAAGCCAAAGATCTATTGGT TTAAAGATGGGAAGCAGATTTCTCCGAAGAGCGATCACTACACCATTCAGAGAGACCTTGATGGGACCTG CTCTCTCCACACCACGGCCTCTACCCTAGACGACGATGGGAACTACACCATCATGGCTGCCAACCCTCAG GGTCGCGTCAGTTGTACAGGAAGGCTAATGGTACAGGCTGTCAACCAAAGAGGCCGCAGTCCCCGCTCTC CCTCAGGCCATCCTCATGCCAGAAGGCCTCGCTCTCGATCACGGGACAGTGGAGATGAAAACGAGCCCAT TCAGGAGCGATTCTTCAGACCTCACTTCCTGCAGGCTCCTGGAGACCTGACCGTTCAGGAAGGCAAGCTC TGCAGGATGGACTGCAAGGTCAGTGGGTTACCAACCCCAGATCTCAGCTGGCAACTAGATGGAAAGCCCA TACGCCCCGACAGTGCTCACAAGATGCTGGTCCGTGAGAATGGGGTCCACTCCCTCATTATAGAGCCAGT CACGTCCCGGGACGCCGGCATCTACACATGTATTGCCACCAACAGAGCAGGACAGAACTCGTTTAACCTG GAGCTTGTGGTTGCTGCTAAGGAAGCACACAAGGCCCCTGTGTTTATGGAGAAGCTACAGAACACGGGGG TTGCTGATGGATACCCAGTGCGGCTGGAATGCCGTGTCTCGGGAGTGCCGCCACCTCAGATATTTTGGAA GAAAGAAAATGAATCGCTCACTCACAGCACTGAGCGAGTAAGCATGCACCAGGATAATCATGGCTACATC TGCCTGCTCATCCAGGGAGCCACAAAGGAAGACGCTGGGTGGTACACTGTGTCCGCCAAGAACGAAGCAG GCATTGTGTCCTGCACTGCCCGGCTGGATGTCTACACCCAGTGGCACCAGCAGCCACAGACCACCAAGCC AAAAAAAGTACGGCCCTCGGCCAGTCGCTACGCAGCACTTTCGGACCAGGGACTAGACATCAAAGCCGCT TTCCAACCTGAAGCCAGCCCATCTCACCTGACTCTGAACAGCGGCTTGGTAGAAAGTGAAGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231473 representing NM_001293773 Red=Cloning site Green=Tags(s) MPQAQKKTTSVSLTIGSSAPKTGVTTAVIQPLSVPVQQAHSATSYLCRPDGTTMGCLLPVFTKELQNTAA SEGQVVVLECRVRGAPPLQVQWFRQGSEIQDSPDFRILQKKPRSTAEPEEICTLVIAESFPEDAGIFTCS ATNDYGSVTSTAQLVITSANNENCSYDSTGEPNSDHFQHFPPPPPILETGSYELASQKPSEIQQVNSPNL GFSMAALQMQFNTAERETNGVHPSHGVNGLINGKAYGNKSPPTPTALLSPTKEPPPLLAKPKLDPLKLQQ LQNQVRLEQEACAWPPAPPGVPCNSSSSGSSAPPSPPFPPPPPAFPELAACASPVPSEPMSALASRATAM QSSGSFNYARPKQFIAAQNLGPASGLPTPTSSPSSSSLPSPLSPTPRPFGRAPGPPFVEPEAMWGPSSPS PPPPPPPVFSPSAAYPVPDVFPLPPPPPPLPSSTSHCASPARFGPSQTPAAFLSALLPSQPPPVAVNALG LPKGVTPAGFPKKSSRTARIASDEEIQGTKDAVIQDLERKLRFKEDLLNNGQPRLTYEERMARRLLGADS ANVFNIQEPEETAANQEYKVSSCEQRLISEIEYRLERSPVDESGDEVQDPDVPVENATAPFFEMKLKHYK IFEGMPVTFTCRVAGNPKPKIYWFKDGKQISPKSDHYTIQRDLDGTCSLHTTASTLDDDGNYTIMAANPQ GRVSCTGRLMVQAVNQRGRSPRSPSGHPHARRPRSRSRDSGDENEPIQERFFRPHFLQAPGDLTVQEGKL CRMDCKVSGLPTPDLSWQLDGKPIRPDSAHKMLVRENGVHSLIIEPVTSRDAGIYTCIATNRAGQNSFNL ELVVAAKEAHKAPVFMEKLQNTGVADGYPVRLECRVSGVPPPQIFWKKENESLTHSTERVSMHQDNHGYI CLLIQGATKEDAGWYTVSAKNEAGIVSCTARLDVYTQWHQQPQTTKPKKVRPSASRYAALSDQGLDIKAA FQPEASPSHLTLNSGLVESEDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293773
ORF Size	3006 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293773.2
RefSeq Size	5458 bp
RefSeq ORF	3009 bp
Locus ID	72333
MW	108.7 kDa
Gene Summary	Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure.[UniProtKB/Swiss-Prot Function]

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