Epha10 (NM_001256432) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A330090H18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231476 representing NM_001256432 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGGCGCCGGTCCACACCCGCTGCGCCTGTTCGTCTGCCTGATACCACTCTGCCTTGCTCTGC TTCTGGGACCTGGGCGCCCCGGGACCGCCGAAGAAGTTATCCTCCTGGACTCCAAAGCCTCCCAGGCTGA GCTGGGCTGGACTGCACTGCCAAGCACTGGGTGGGAGGAGATTAGTGGCGTGGATGAACACGACCGTCCC ATCCGCACGTACCAAGTGTGCAACGTGCTGGAGCCCAACCAGGACAACTGGCTTCAGACAGGCTGGATCA GCCGCGGCCGCGGGCAGCGCATCTTCGTGGAGCTGCAGTTCACACTGCGCGACTGCAGTAGCATACCAGG TGCCACGGGCACCTGCAAAGAGACCTTCAACGCATACTACCTAGAAACCGAGACCGACCTGGGCCGAGGG CGTCCCCGCCTAGGAGGCAACCGACCCCGCAAGATTGACACCATCGCAGCTGATGAGAGCTTCACGCAGG GTGACCTGGGGGAGCGAAAGATGAAGCTGAACACAGAGGTGCGTGAGATCGGCCCCCTCAGCCGGCAAGG CTTCCACCTGGCCTTCCAGGACGTGGGTGCGTGCGTGGCGTTGGTCTCGGTGCGTGTCTACTACAAGCAG TGCCGCGCCACAGTGCGGGGCCTGGCAGCCTTCCCCGCCACCGCAGCTGAGAGCGCCTTCTCCACGCTGG TGGAAGTGGCTGGAACGTGCGTGGCCCACTCGGAAGGGGAGCCCAGCAGCCCTCCCCGCATGCACTGTGG TGCCGATGGCGAGTGGCTGGTGCCTGTGGGCCGTTGCAGCTGCAGTGCAGGATTCCAGGAACATGGTGAC ATCTGTGAAGCTTGTCCCCCAGGGTTTTACAAGGTGTCCCCAAGGAGGCCCCTCTGCTCGCCCTGCCCGG AGCACAGCTTGGCCCTGGAGAATGCTTCTACATTCTGTGTGTGCCAGGACACCTATGCTCGCTCGCCCAC CGACCCGCCCTCAGCTTCCTGCACCCGGCCGCCGTCCGCGCCGCGGGACCTGCAGTACAGCCTGAGCCGC TCCCCGCTGGCGCTGCGGCTGCGCTGGCTGCCGCCTGCGGACTCAGGCGGCCGCTCCGACGTCACCTACT CGCTGCTGTGCCTGCGCTGCGGGCGCGACGGTCCAGCGGGCGCGTGCCAACCCTGCGGGCCGCGCGTGGC CTTCGTCCCGCGCCAGGCTGGGCTGCGGGAGCGCGCCGCCACGCTGCTGCACCTGCGGCCGGGCGCGCGC TACACCGTGCGCGTGGCCGCGCTCAACGGCGTCTCAGGCCCGGCTGCCGCCGCGGGAGCCACCTATGCGC AGGTCACAGTGTCCACCGGACCCGGGGCACCATGGGAAGAGGATGAAATCCGCAGGGACCGCGTGGAGCC CCAAAGCGTATCGCTGTCGTGGCGAGAGCCTGTGCCTGCTGGAGCTCCTGGAACCAACAGCACAGAGTAT GAGATCCGCTACTATGAGAAGGGTCAGAGTGAGCAGACTTACTCCACAGTGAAGACAGGAGCACCGGCTG TCACTGTCACCAACCTGAAGCCTGCTACCAGATACGTTTTTCAGATCCGGGCGGCCTCCCCAGGACCTTT GTGGGAGGCCCAGAGCTTCAGTCCAAGCATTGAAGTGCAGACTCCAGGGGAAGTTGCCCCAGGGTCCAGG GACCAGAGCCCTGCAGTGGTTGTCACCGTGGTGACCATCTCAGCCCTCTTGGTCCTGGGCTCCGTGATGA GTGTGTTGGCCATCTGGAGGAGGCCCTGCGATGGCAAAGGAAGTGGCAACGCCCATGACGAAGAGGAACT GTATTTCCACTTCAAAGTCCCGACACGACGCACGTTCCTGGACCCCCAAAGCTGCGGGGACCCGTTGCAG GCTGTGCATCTGTTTGCCAAAGAACTAGATGCCAAAAGCGTCACCCTGGAGAAGAGCCTGGGAGCAGGAC GATTTGGGGACCTGTGCTGCGGCTGCCTGCAGCTGCCCGGTCGCCAGGAACTGCCCGTGGCCGTGCACAC TCTGAGGGATGGCTGCTCAGACTCCCAGAGGCTCAGCTTCCTGGCTGAGGCTCTCACCCTGGGGCAGTTT GACCATAGCCACATTGTGCGGCTGGAAGGCGTTGTCACCCGAGGAAACCCCTTGATGATTGTCACTGAGT ACATGAACCTCGGGGCCCTTGATGACTTCCTCAGGCATCACGAGGGGGAGCTGGTGGCTGCGCAGCTGAT GGGTCTGCTGCCTGGGCTGGCATCGGCCATGAAGTATCTGTCGGAGATGGGCTACGTTCACCGGGGCCTT GCAGCCCGCCGAGTGCTTGTCAGCAGTGGCCTCCTCTGTAAGATCTCTGGCTTTGGGCGTGGTCCCCGGG ACCGAGCAGAAGCTGTCTACACTACCATGAGTGGCAGGAGCCCAGCGCTCTGGGCAGCTCCTGAGACTCT ACAGTTTGGCCACTTCAGTTCTGCTAGTGATGTGTGGAGCTTTGGCATTGTCATGTGGGAGGTGATGGCC TTTGGGGAGCGGCCCTACTGGGATATGTCTGGACAAGATGTAATCAAGGCCGTGGAGGATGGCTTCCGGC TGCCGCCCCCCAGGAACTGTCCCTCCCAACTGCATCGACTGATGCTCGAGTGTTGGCAGAAAGATCCAAG TGAACGGCCCAGGTTTTCCCAGATCCACAGCATCCTGAGCAAGATGGGGCAGGAGCCAGAACCCTCCAAG TGTGCCTCGACCACCTGTCTCAGGCCTCCCACACCCCTGGCAGACCGTGCCTTTTCTACCTTCCCCTCCT TTGGCTCCGTGGGTGCCTGGCTGGAGGCCCTAGACCTGTGCCGCTACAAGGACAACTTCTCTGCAGCGGG CTACGGGAGCCTAGAGGCCGTGGCTGAGATGACTGCCCAGGACCTGGGGAGCCTTGGCATCTCTTCCGCA GAGCATCGAGAAGCCCTCCTTAGTGGGATCAGTGCCCTGCAGACTCGAGTGCTACAACTACAGGGTCAGG GGGTGCAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231476 representing NM_001256432 Red=Cloning site Green=Tags(s) METGAGPHPLRLFVCLIPLCLALLLGPGRPGTAEEVILLDSKASQAELGWTALPSTGWEEISGVDEHDRP IRTYQVCNVLEPNQDNWLQTGWISRGRGQRIFVELQFTLRDCSSIPGATGTCKETFNAYYLETETDLGRG RPRLGGNRPRKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSRQGFHLAFQDVGACVALVSVRVYYKQ CRATVRGLAAFPATAAESAFSTLVEVAGTCVAHSEGEPSSPPRMHCGADGEWLVPVGRCSCSAGFQEHGD ICEACPPGFYKVSPRRPLCSPCPEHSLALENASTFCVCQDTYARSPTDPPSASCTRPPSAPRDLQYSLSR SPLALRLRWLPPADSGGRSDVTYSLLCLRCGRDGPAGACQPCGPRVAFVPRQAGLRERAATLLHLRPGAR YTVRVAALNGVSGPAAAAGATYAQVTVSTGPGAPWEEDEIRRDRVEPQSVSLSWREPVPAGAPGTNSTEY EIRYYEKGQSEQTYSTVKTGAPAVTVTNLKPATRYVFQIRAASPGPLWEAQSFSPSIEVQTPGEVAPGSR DQSPAVVVTVVTISALLVLGSVMSVLAIWRRPCDGKGSGNAHDEEELYFHFKVPTRRTFLDPQSCGDPLQ AVHLFAKELDAKSVTLEKSLGAGRFGDLCCGCLQLPGRQELPVAVHTLRDGCSDSQRLSFLAEALTLGQF DHSHIVRLEGVVTRGNPLMIVTEYMNLGALDDFLRHHEGELVAAQLMGLLPGLASAMKYLSEMGYVHRGL AARRVLVSSGLLCKISGFGRGPRDRAEAVYTTMSGRSPALWAAPETLQFGHFSSASDVWSFGIVMWEVMA FGERPYWDMSGQDVIKAVEDGFRLPPPRNCPSQLHRLMLECWQKDPSERPRFSQIHSILSKMGQEPEPSK CASTTCLRPPTPLADRAFSTFPSFGSVGAWLEALDLCRYKDNFSAAGYGSLEAVAEMTAQDLGSLGISSA EHREALLSGISALQTRVLQLQGQGVQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256432
ORF Size	3021 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256432.2
RefSeq Size	5186 bp
RefSeq ORF	3024 bp
Locus ID	230735
MW	109.5 kDa
Gene Summary	Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5 (By similarity).[UniProtKB/Swiss-Prot Function]

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