Egflam (NM_001289496) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5930412K08; AU040377
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231488 representing NM_001289496 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTAATCTCAACCTTCTCGCTCCATTTCTTGCTCCTGGCCTGCAGCCTCCCACCCGGCGCTGTGT CACTCCGAACGGCTCTGCGAAAATCAGGCAAGGTGGGACCTCCTCTTGACATCAAGCTGGGAGCCTTGAA CTGTACAGCTTTCAGCATCCAGTGGAAAACACCAAAGCGTTCTGGTAGCTCCATCATTGGCTACACAGTG TTTTACTCTGAGGTTGGTTCAGATAAGTCCCTGCGGGAGCGGTCACACAACGTCCCGGTTGGCCAGGATA CCCTAATCACTGAGGAAGTGATTGGAGATTTGAAGCCAGGCACTGAATATCAAGTAAGCGTAGCAGCCTA CAGTCAGACTGGCAAGGGGAGGCTTAGCTTCCCTCGACATGTGACCACTTTGTCTCAAGATTCCTGCCTG CCCCCTGCAGCACCCCAACAGCCACATGTCCTTGTGGTTTCCGATTCTGAAGTGGCCCTTTCTTGGAGAC CTGGAGAGAATGAAGGAAGTGCCCCAATTCAGTCCTACTCTGTGGAATTCATTAGACCAGATTTTGACAA GAGCTGGACCATAATCCAAGAGCGCCTGCAGATGGACTCCATGGTTATCAAAGGCCTTGATCCAGATACC AACTACCAGTTTGCTGTGAAAGCCATGAATGCTCACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCG TCCGAACCCTGGGTCCCGGGGAGGCTGGAAGTGGTCATTATGGACCTGGGTATATCACCAATCCCGGTGT GAGTGAAGATGATGATGGATCTGAAGACGAACTGGACCTGGATGTATCCTTTGAGGAGGTTAAACCACTT CCTGCTACCAAAGTTGGGAATAAGAAGTTCTCAGTGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGA TGGGCTCCAGACTCGCCCAACCTACCTCAGCCTCTCTCCACGAAACTACAGTGGCCATCCCACCCACACC AGCCCAACGGAAGGGGAAGAACAGTGTGGCCATGATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACT CTCTGTTCAGCTGACAGCTTCTGTGTCAATGACTACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGG GCAAAGGAGGCGAGGCCTGCTCAGAAGATATCTTTATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGT AACCTTTGAGCCTCTGAAGAACTCTTATCAGGCATTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAG GATGGCTTGCTGCTCTACTGCGGAGAGAGTGAGCACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCC GGCGGTCCCTCCACTTCAGGTTTAATTGTGGAACTGGGATTGCCATCATCATAAGTGAGACCAAAATCAA ACTTGGGGCCTGGCATACGGTGACACTGTACAGAGATGGCCTGAATGGGATGCTGCAGTTGAATAATGGC ACCCCAGTGACAGGCCAGTCCCAGGGTCAGTACAGTAAAATTACCTTCCGGACACCTCTGTACCTCGGGG GCGCTCCCAGTGCTTACTGGCTGGTCAGAGCAACAGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTC ACTTTCTGTTAATGGGAAGAAGATTGACATGAGGCCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGAT GTTGGGGAGTGCAGCAGTGGGATCTGTGACGAAGCCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCA AGGCCGACTCCTACATCTGCCTCTGTCCCCTTGGGTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCT GACCATCCCTCAGTTCAGAGAGTCGCTGAGGTCCTATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCAT TATCTTTCTTTCACCGAGTTCGAGATCACCTTTCGGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCT ACGACACGGGCAGCAAGGACTTCCTCTCCATCAATATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTG TGGTTCTGGGACGGGAGTTCTCAGGAGTGAGGCTCCCCTCACCCTAGGCCAATGGCATGACCTACGTGTG TCTCGCACAGCGAAGAATGGGATCTTACAGGTGGACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAG GCTTCACCCAGATTAAGTGTAATACGGACATTTTTATTGGTGGAGTCCCAAATTACGATGATGTCAAGAA GAACTCGGGGATCCTCCATCCATTTAGTGGGAGCATTCAGAAGATCATCCTCAACGACCGGACCATCCAT GTGAAGCATGACTTTACATCGGGCGTGAATGTGGAGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTG CCCATGGGGGCAGCTGTCGGCCCAGGAAGGAGGGTTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCT GAACTGCCAGAAAGAGTGTGGGAACCACTGCCTCAATACTATTATAGAAGCCATCGAGATCCCACAGTTC ATCGGCCGCAGCTACCTGACGTATGACAACCCAAATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATG CGTTCATGAGGTTTAAGACAACCGCCAAGGATGGCCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACC TAACAGTGACTTCATTTCCCTGGGTCTTCGGGATGGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGC GTGGCTTCCATCATGGTGAACGGTTCCTTCAGCGATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATG GCCAGTCCGGGAAGATTACAGTGGATGACTACGGAGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCA GCTTAACATCAACGGTGCTCTGTACGTGGGTGGAATGAAGGAAATCGCTCTGCACACGAACAGACAATAC CTGCGAGGCCTTGTGGGCTGCATCTCCCATTTCACACTGTCCACCGATTACCACATTTCCCTCGTGGAAG ACGCCGTGGATGGGAAGAACATCAACACTTGTGGGGCTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231488 representing NM_001289496 Red=Cloning site Green=Tags(s) MDLISTFSLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIIGYTV FYSEVGSDKSLRERSHNVPVGQDTLITEEVIGDLKPGTEYQVSVAAYSQTGKGRLSFPRHVTTLSQDSCL PPAAPQQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDT NYQFAVKAMNAHGFSPRSWPSNTVRTLGPGEAGSGHYGPGYITNPGVSEDDDGSEDELDLDVSFEEVKPL PATKVGNKKFSVESKKTSVSNSVMGSRLAQPTSASLHETTVAIPPTPAQRKGKNSVAMMSRLFDMSCDET LCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAE DGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNG TPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGAD VGECSSGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQH YLSFTEFEITFRPDSGDGVLLYSYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRV SRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIH VKHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQF IGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSG VASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQY LRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289496
ORF Size	3051 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289496.1, NP_001276425.1
RefSeq Size	4888 bp
RefSeq ORF	3054 bp
Locus ID	268780
UniProt ID	Q4VBE4
MW	111.2 kDa
Gene Summary	Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.