Llgl2 (NM_001252532) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9130006H11Rik; Llglh2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231526 representing NM_001252532 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGTTCCTGAGAACTGGACATGACCCTGCCCGGGAGAGGCTGAAGCGGGATCTGTTCCAGTTTA ACAAGACAGTGGAGCATGGCTTCCCACACCAGCCCAGCGCCCTTGGCTATAGCCCTTCGCTGCGCATCCT GGCCATCGGAACCCGCTCTGGAGCTGTCAAGCTATATGGCGCCCCCGGGGTAGAGTTCATGGGCCTTCAC AAGGAGAACAACGCAGTGCTGCAGATCCACTTCCTGCCTGGTCAGTGTCAGCTGGTCACTCTGCTGGATG ACAATAGCCTGCACCTGTGGAGCCTGAAGGTCAAGGGCGGGGTGTCAGAGCTGCAGGAAGAAGAGAGCTT CACATTGCGTGGCCCCCCAGGGGCTGCCCCCAGTGCCACGCAGGTCACTGAGATTCTACCTCACTCTTCC GGAGAACTGCTCTACCTGGGCACCGAGAGCGGCAACGTGTTTGTGGTGCAGCTCCCGGGGTTCCGCACGC TGCACGACAGGACCATCTGTTCAGATGAGGTGCTGCAATGGTTGCCAGAGGAGGCTCGCCACCGGCGAGT GTTTGAGATGGTGGAGGCTCTGCAGGAGCACCCTCGAGACCCCAACCAAATCCTCATTGGCTACAGCCGA GGCCTCGTCGTCATCTGGGACCTTCAGGGCAGCCGAGCGCTCAGTCATTTCCTCAGCAGCCAGCAACTGG AGAATGCCAGCTGGCAGCGGGATGGCTGCCTGATTGTCACCTGCCACTCCGACGGCAGCCACTGCCAGTG GCCCGTCTCCAGTGACACCCAGAACCCAGAACCTCTGCGCAGCTCTATACCTTACGGTCCCTTTCCTTGC AAAGCTATAACGAAAATCTTCTGGCTCACCACCAGGCAAGGGTTGCCCTTCACCATCTTCCAGGGCGGTA TGCCACGGGCCAGCTATGGGGACCGCCACTGCATCTCAGTGGTCCACAACGGCCAGCAGACGGCCTTCGA CTTCACCTCCCGTGTCATTGACTTCACTGTTCTCTCAGAGGCTGACCCTGCGGCTGCCTTTGATGACCCC TACGCCCTGGTGGTGCTAGCCGAGGAGGAACTGGTAGTCATTGACCTGCAGACACCCGGCTGGCCACCAG TCCAGCTGCCCTACCTGGCTTCCTTGCACTGTTCCGCTATCACCTGCTCCCACCACGTCTCCAACATCCC CCTGAAGCTATGGGAACGCATCATCGCCGCGGGCAGCCGCCAGAACTCACACTTCTCCACCATGGAGTGG CCCATAGATGGTGGCACCAGCCTGGCCCCACCGCCACCCCAGAGGGACCTGCTGCTCACAGGGCACGAGG ACGGCACGGTGCGGTTCTGGGACGCCTCCGGTGTGTGCTTACGTCTGCTCTACAAACTGAGCACTGTGAG GGTGTTCCTCACAGACACGGACCCCAGCGAGAACCTCATTGCCCAGGGTGAGGACGAGTGGCCCCCACTC CGCAAGGTGGGCTCCTTTGATCCCTACAGCGATGATCCGCGGCTAGGCATCCAGAAGATTTTCCTCTGCA AATACAGCGGCTACCTGGCTGTGGCAGGCACGGCAGGGCAGGTGCTGGTGCTGGAGTTGAACGATGAGGC GGCCGAGCATGCTGTGGAGCAGGTGGAGGCTGACCTGCTGCAGGACCAGGAGGGTTACCGCTGGAAGGGG CACGAGCGCCTCGCCGCCCGCCCGGGGCCCGTGTGCTTTGAGGCGGGATTTCAGCCCTTTGTACTGGTGC AGTGCCAGCCCCCAGCTGTGGTCACCTCCTTGGCTCTGCACTCCGAGTGGCGGCTTGTGGCCTTTGGCAC CAGCCATGGTTTCGGCCTCTTCGATCACCAGCAACGGCGGCAGGTCTTTGTCAAGTGCACACTTCACCCC AGTGACCAGCTGGCCTTGGAGGGCCCTCTGTCTCGAGTAAAGTCCCTCAAGAAGTCTCTACGTCAGTCAT TCCGTCGGATGCGTCGCAGCAGAGTGTCCAGTCATAAACGGCGGCCTGGTGGCCCCACAGGCGAGGTGAG GCCCCAGGCTCAGAGCAGCCATCTACAGGACCATACAGCTCTCCCTCAGGATCTGAATCCAGATCAAGCG CAGGCCCAGGCTGTGAATATCAAGGCTGAGCGGACGGGCCTGCAGAACATGGAGTTGGCGCCCGTGCAAC GGAAGATTGAGGCTCGCTCCGCGGAGGACTCCTTCACTGGCTTTGTCCGGACCCTCTATTTCGCTGATAC CTACCTGAGGGACAGTTCCCGCCACTGTCCCTCCCTGTGGGCTGGCACCAACGGAGGCACCGTCTACGCC TTTTCCCTGCGCGTGCCTCCTGCAGAAAGAAGAACAGATGAACCTGTCCGGGCAGAGCAGGCCAAGGAGA TCCAGCTGATGCATCGCGCGCCTGTGGTGGGCATCCTGGTGCTCGACGGACACAACGTGCCCCTCCCCGA GCCCCTGGAAGTCGCCCATGACCTGTCGAAGAGCCCAGACATGCAAGGGAGCCACCAATTGCTTGTGGTG TCGGAGGAGCAATTCAAGGTATTCACACTGCCCAAGGTGAGCGCCAAACTGAAGCTGAAGCTGACGGCCC TGGAGGGCTCACGGGTGCGGCGAGTCGGTGTCGCCCACTTTGGCAGCTGCAGGGCTGAGGACTATGGGGA GCACCACCTGGCAGTGCTCACCAACTTGGGTGACATCCAGGTGGTCTCAATGCCCCTGCTCAAGCCCCAG GTGCGGTACAGCTGCATCCGCCGGGAGGACGTCAGCGGCATAGCTTCCTGTGTCTTCACCAAATATGGCC AAGGTTTCTATCTGATATCACCTTCGGAGTTCGAGCGCTTTTCTCTCTCCACCAAGTGGCTGGTTGAACC CCGGTGTTTGGTGGATTCAACCAAAGCCAAGAAGCACAACCGGCCCAGCAATGGCAATGGCACAGGCCTC AAAATGACCTCCTCAGGCCACGTCAGGAACTCAAAGAGCCAAAGTGATGGAGATGAGAAGAAGCCGGGCC CAGTGATGGAGCACGCGCTGCTCAATGACGCCTGGGTCCTAAAGGAAATCCAGAGCACGCTGGAGGGGGA CCGGAGGAGCTATGGCAATTGGCATCCTCACCGCGTGGCTGTGGGTTGCAGGCTCAGCAATGGAGAAGCA GAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231526 representing NM_001252532 Red=Cloning site Green=Tags(s) MRRFLRTGHDPARERLKRDLFQFNKTVEHGFPHQPSALGYSPSLRILAIGTRSGAVKLYGAPGVEFMGLH KENNAVLQIHFLPGQCQLVTLLDDNSLHLWSLKVKGGVSELQEEESFTLRGPPGAAPSATQVTEILPHSS GELLYLGTESGNVFVVQLPGFRTLHDRTICSDEVLQWLPEEARHRRVFEMVEALQEHPRDPNQILIGYSR GLVVIWDLQGSRALSHFLSSQQLENASWQRDGCLIVTCHSDGSHCQWPVSSDTQNPEPLRSSIPYGPFPC KAITKIFWLTTRQGLPFTIFQGGMPRASYGDRHCISVVHNGQQTAFDFTSRVIDFTVLSEADPAAAFDDP YALVVLAEEELVVIDLQTPGWPPVQLPYLASLHCSAITCSHHVSNIPLKLWERIIAAGSRQNSHFSTMEW PIDGGTSLAPPPPQRDLLLTGHEDGTVRFWDASGVCLRLLYKLSTVRVFLTDTDPSENLIAQGEDEWPPL RKVGSFDPYSDDPRLGIQKIFLCKYSGYLAVAGTAGQVLVLELNDEAAEHAVEQVEADLLQDQEGYRWKG HERLAARPGPVCFEAGFQPFVLVQCQPPAVVTSLALHSEWRLVAFGTSHGFGLFDHQQRRQVFVKCTLHP SDQLALEGPLSRVKSLKKSLRQSFRRMRRSRVSSHKRRPGGPTGEVRPQAQSSHLQDHTALPQDLNPDQA QAQAVNIKAERTGLQNMELAPVQRKIEARSAEDSFTGFVRTLYFADTYLRDSSRHCPSLWAGTNGGTVYA FSLRVPPAERRTDEPVRAEQAKEIQLMHRAPVVGILVLDGHNVPLPEPLEVAHDLSKSPDMQGSHQLLVV SEEQFKVFTLPKVSAKLKLKLTALEGSRVRRVGVAHFGSCRAEDYGEHHLAVLTNLGDIQVVSMPLLKPQ VRYSCIRREDVSGIASCVFTKYGQGFYLISPSEFERFSLSTKWLVEPRCLVDSTKAKKHNRPSNGNGTGL KMTSSGHVRNSKSQSDGDEKKPGPVMEHALLNDAWVLKEIQSTLEGDRRSYGNWHPHRVAVGCRLSNGEA E TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252532
ORF Size	3153 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252532.1, NP_001239461.1
RefSeq Size	3629 bp
RefSeq ORF	3156 bp
Locus ID	217325
UniProt ID	Q3TJ91
MW	117.5 kDa
Gene Summary	Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity (By similarity).[UniProtKB/Swiss-Prot Function]

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