Daam1 (NM_001286452) Mouse Tagged ORF Clone
CAT#: MR231543
- TrueORF®
Daam1 (myc-DDK-tagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 3
"NM_001286452" in other vectors (1)
Need custom modification / cloning service?
Get a free quote
CNY 10,640.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231543 representing NM_001286452
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCACGGAAGAGAGGCGGCCGGGGGATTTCATTTATCTTTTGCTGTTTCCGAAACAATGACCATC CAGAAATCACATATCGGCTTAGGAATGATAGCAACTTTGCACTTCAGACAATGGAGCCGGCGCTGCCCAT GCCCCCTGTGGAGGAGCTCGATGTCATGTTCAGTGAACTTGTGGATGAACTTGACCTCACAGACAAACAC AGGGAAGCCATGTTTGCACTTCCAGCAGAGAAAAAGTGGCAGATATACTGTAGCAAGAAAAAGGACCAGG AAGAAAACAAGGGAGCAACCAGTTGGCCCGAGTTCTACATTGATCAGCTCAATTCCATGGCTGCTAGGAA ATCTCTGCTGGCTTTAGAGAAGGAAGAAGAGGAGGAGAGGAGTAAAACCATCGAGAGTCTGAAGACAGCA CTGAGAACCAAGCCCATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTGTCATGTATTCTCAACT TTCTGAAGACCATGGACTATGAGACCTCTGAGTCTCGGATACACACCTCCCTCATTGGCTGTATAAAGGC ACTAATGAACAACTCTCAAGGCCGGGCCCATGTCCTGGCTCATTCTGAGAGTATCAATGTAATTGCTCAG AGTCTGAGCACAGAGAACATTAAAACAAAGGTGGCCGTGCTGGAAATCCTGGGCGCTGTGTGCCTGGTTC CTGGGGGCCACAAGAAAGTTCTGCAAGCTATGCTGCACTACCAGAAGTATGCCAGCGAGAGAACCCGCTT CCAGACGTTAATTAATGACTTGGATAAAAGCACTGGGCGTTATCGGGATGAAGTAAGTCTCAAGACGGCC ATCATGTCCTTCATCAATGCAGTGCTGAGCCAGGGAGCCGGAGTGGAGAGTTTGGACTTCAGGCTCCATC TTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTTATAGATAAATTAAGGGAACATGAAAATTCAAC ACTAGACAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTT GAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACA GCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAA CACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAG GATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATG AAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGA GAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATG AAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGC AGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGG AGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGA GCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAG GAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCAC CAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAA ATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAA GACAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTCCAAACTTAAAGTCAAAGAGCTGTCAGT GATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATC AAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAGCTTTTGAAAT TCGTTCCTGAGAAAAGCGACATCGACCTGCTAGAGGAACACAAGCATGAGCTGGATCGGATGGCCAAGGC TGACCGCTTCCTGTTTGAGATGAGCAGAATTAATCATTACCAGCAAAGACTGCAATCACTGTACTTCAAA AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAGCCCAAAGTAGAAGCGATTCGCTCTGGCTCTGAGGAGG TGTTCAGGAGCCGCGCCCTCAAGCAGCTCCTGGAGGTGGTCTTGGCTTTTGGAAATTATATGAATAAAGG ACAAAGAGGCAATGCATATGGATTCAAGATCTCCAGCCTCAACAAGATAGCGGATACCAAATCCAGTATT GACAAGAACATTACTCTTTTGCACTATCTAATAACTATTGTGGAGAATAAGTACCCCAAAGTCCTCAACC TAAGCGAAGAGTTGCGGGATATTCCTCAAGCAGCGAAAGTAAACATGACTGAGCTGGACAAGGAGATCAG CACCCTGAGGAGTGGCCTGAAAGCTGTAGAGACGGAGCTGGAGTATCAGAAGTCTCAGCCCCCACAGCCT GGAGACAAGTTCGTGTCTGTTGTCAGCCAGTTCATCACCCTGGCTAGCTTCAGCTTCTCAGATGTTGAAG ATCTTCTAGCAGAAGCTAAAGAGCTGTTCACTAAAGCAGTGAAGCACTTTGGGGAAGAGGCTGGCAAGAT CCAGCCAGATGAGTTCTTCGGGATCTTTGATCAGTTTCTTCAGGCCGTGGCCGAAGCGAAACAGGAAAAT GAGAACATGAGAAAAAGGAAGGAGGAGGAGGAACGGCGGGCTCGCCTGGAAGCTCAGCTCAAAGAGCAGC GGGAGAGGGAGCGGAAGGTGAGGAAGGCCAAGGAGAGCAGTGAAGAGAGTGGAGAGTTTGACGACCTGGT CTCGGCGCTGCGCTCAGGAGAGGTGTTCGACAAAGACCTTTCAAAACTCAAACGGAACCGGAAACGCATT TCCAACCAGGTGACGGACAGCAGCCGGGAGCGACCAATCACAAAACTTAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231543 representing NM_001286452
Red=Cloning site Green=Tags(s) MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKH REAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTA LRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQ SLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTA IMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRF ELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQ DPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKM KEKLEKETTEHKQVKQQVADLTAQLHELNRRAVCAAVPGGPSPGAPGGPFPSSGLGSLLPPPPPPLLSGG ALPPPPPPLPPGGPPPPPGPPPLGGVLPPPGAPVSLTLKKKNIPQPTNALKSFNWSKLPENKLDGTVWTE IDDTKVFKILDLEDLERTFSAYQRQQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEI KRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFK KKFAERVAEVKPKVEAIRSGSEEVFRSRALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSI DKNITLLHYLITIVENKYPKVLNLSEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQP GDKFVSVVSQFITLASFSFSDVEDLLAEAKELFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVAEAKQEN ENMRKRKEEEERRARLEAQLKEQRERERKVRKAKESSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRI SNQVTDSSRERPITKLNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001286452 |
ORF Size | 3204 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286452.1, NP_001273381.1 |
RefSeq Size | 6028 bp |
RefSeq ORF | 3207 bp |
Locus ID | 208846 |
UniProt ID | Q8BPM0 |
MW | 122.7 kDa |
Gene Summary | Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229332 | Daam1 (untagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 3 |
CNY 10,260.00 |