Daam1 (NM_001286452) Mouse Untagged Clone

CAT#: MC229332

Daam1 (untagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 3



  "NM_001286452" in other vectors (1)

CNY 10,260.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229332 representing NM_001286452
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCACGGAAGAGAGGCGGCCGGGGGATTTCATTTATCTTTTGCTGTTTCCGAAACAATGACCATC
CAGAAATCACATATCGGCTTAGGAATGATAGCAACTTTGCACTTCAGACAATGGAGCCGGCGCTGCCCAT
GCCCCCTGTGGAGGAGCTCGATGTCATGTTCAGTGAACTTGTGGATGAACTTGACCTCACAGACAAACAC
AGGGAAGCCATGTTTGCACTTCCAGCAGAGAAAAAGTGGCAGATATACTGTAGCAAGAAAAAGGACCAGG
AAGAAAACAAGGGAGCAACCAGTTGGCCCGAGTTCTACATTGATCAGCTCAATTCCATGGCTGCTAGGAA
ATCTCTGCTGGCTTTAGAGAAGGAAGAAGAGGAGGAGAGGAGTAAAACCATCGAGAGTCTGAAGACAGCA
CTGAGAACCAAGCCCATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTGTCATGTATTCTCAACT
TTCTGAAGACCATGGACTATGAGACCTCTGAGTCTCGGATACACACCTCCCTCATTGGCTGTATAAAGGC
ACTAATGAACAACTCTCAAGGCCGGGCCCATGTCCTGGCTCATTCTGAGAGTATCAATGTAATTGCTCAG
AGTCTGAGCACAGAGAACATTAAAACAAAGGTGGCCGTGCTGGAAATCCTGGGCGCTGTGTGCCTGGTTC
CTGGGGGCCACAAGAAAGTTCTGCAAGCTATGCTGCACTACCAGAAGTATGCCAGCGAGAGAACCCGCTT
CCAGACGTTAATTAATGACTTGGATAAAAGCACTGGGCGTTATCGGGATGAAGTAAGTCTCAAGACGGCC
ATCATGTCCTTCATCAATGCAGTGCTGAGCCAGGGAGCCGGAGTGGAGAGTTTGGACTTCAGGCTCCATC
TTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTTATAGATAAATTAAGGGAACATGAAAATTCAAC
ACTAGACAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTT
GAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACA
GCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAA
CACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAG
GATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATG
AAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGA
GAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATG
AAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGC
AGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGG
AGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGA
GCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAG
GAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCAC
CAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAA
ATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAA
GACAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTCCAAACTTAAAGTCAAAGAGCTGTCAGT
GATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATC
AAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAGCTTTTGAAAT
TCGTTCCTGAGAAAAGCGACATCGACCTGCTAGAGGAACACAAGCATGAGCTGGATCGGATGGCCAAGGC
TGACCGCTTCCTGTTTGAGATGAGCAGAATTAATCATTACCAGCAAAGACTGCAATCACTGTACTTCAAA
AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAGCCCAAAGTAGAAGCGATTCGCTCTGGCTCTGAGGAGG
TGTTCAGGAGCCGCGCCCTCAAGCAGCTCCTGGAGGTGGTCTTGGCTTTTGGAAATTATATGAATAAAGG
ACAAAGAGGCAATGCATATGGATTCAAGATCTCCAGCCTCAACAAGATAGCGGATACCAAATCCAGTATT
GACAAGAACATTACTCTTTTGCACTATCTAATAACTATTGTGGAGAATAAGTACCCCAAAGTCCTCAACC
TAAGCGAAGAGTTGCGGGATATTCCTCAAGCAGCGAAAGTAAACATGACTGAGCTGGACAAGGAGATCAG
CACCCTGAGGAGTGGCCTGAAAGCTGTAGAGACGGAGCTGGAGTATCAGAAGTCTCAGCCCCCACAGCCT
GGAGACAAGTTCGTGTCTGTTGTCAGCCAGTTCATCACCCTGGCTAGCTTCAGCTTCTCAGATGTTGAAG
ATCTTCTAGCAGAAGCTAAAGAGCTGTTCACTAAAGCAGTGAAGCACTTTGGGGAAGAGGCTGGCAAGAT
CCAGCCAGATGAGTTCTTCGGGATCTTTGATCAGTTTCTTCAGGCCGTGGCCGAAGCGAAACAGGAAAAT
GAGAACATGAGAAAAAGGAAGGAGGAGGAGGAACGGCGGGCTCGCCTGGAAGCTCAGCTCAAAGAGCAGC
GGGAGAGGGAGCGGAAGGTGAGGAAGGCCAAGGAGAGCAGTGAAGAGAGTGGAGAGTTTGACGACCTGGT
CTCGGCGCTGCGCTCAGGAGAGGTGTTCGACAAAGACCTTTCAAAACTCAAACGGAACCGGAAACGCATT
TCCAACCAGGTGACGGACAGCAGCCGGGAGCGACCAATCACAAAACTTAATTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001286452
Insert Size 3207 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286452.1, NP_001273381.1
RefSeq Size 6028 bp
RefSeq ORF 3207 bp
Locus ID 208846
UniProt ID Q8BPM0
Gene Summary Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...