Adcy9 (NM_001291910) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AC9; ACtp10; AW125421; D16Wsu65e; mKIAA0520
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231592 representing NM_001291910 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTACCTCTGTACCTGAGCTTGTTTTTGGGGGTGGTCTATTCTGTCCTTTTTGAGACCTTCGGCT ACCACTTCCGAAACGAAGACTGCTACCCTTCTCCGGGCCCTGGGGCCCTGCACTGGGAGCTGCTGAGCAG AGCCCTGCTTCACGTGTGCATTCACGCTATCGGGATCCATCTGTTTGTCATGTCTCAGGTGAGGTCCAGG AGCACCTTTCTCAAGGTGGGACAATCCATTATGCACGGCAAAGATCTGGAAGTAGAGAAAGCCCTGAAAG AGAGGATGATTCATTCAGTGATGCCAAGAATCATAGCCGACGACTTAATGAAACAAGGGGACGAGGAGAG TGAGAATTCCGTCAAGAGGCATGCCACCTCCAGTCCCAAGAACAGGAAGAAGAAGTCCTCCATACAGAAG GCACCGATAGCATTCCGCCCCTTTAAGATGCAGCAGATTGAAGAAGTCAGTATTTTATTTGCAGACATTG TGGGTTTCACCAAGATGAGCGCCAACAAATCTGCGCATGCCTTGGTAGGCCTACTCAATGACCTGTTCGG TCGCTTTGACCGCCTGTGTGAGCAGACCAAGTGTGAGAAGATCAGCACTCTGGGGGACTGTTATTACTGT GTGGCAGGGTGTCCGGAGCCCCGGGCAGACCATGCCTACTGCTGCATTGAAATGGGCTTAGGCATGATAA AAGCCATCGAGCAGTTCTGCCAGGAGAAGAAAGAGATGGTGAACATGCGTGTTGGGGTTCACACGGGGAC TGTCCTGTGTGGCATCCTGGGCATGAGGAGGTTTAAATTTGATGTGTGGTCCAACGATGTGAACTTGGCT AATCTCATGGAGCAGCTGGGAGTGGCTGGCAAAGTTCACATATCTGAGGCCACTGCAAAATACTTAGACG ACAGGTATGAAATGGAAGATGGGAGAGTTATTGAGCGCCTTGGGCAGAGTGTGGTGGCTGACCAGTTGAA AGGTTTGAAGACATACCTGATATCGGGTCAGAGAGCCAAGGAGTCCCACTGCAGCTGTGCAGAGGCCCTG CTTTCTGGCTTTGAGGTCATTGACGACTCACGGGAGTCCTCAGGCCCTAGGGGACAGGGGACAGCATCGC CAGGGAGTGTCAGTGATTTGGCGCAGACTGTCAAAACCTTTGATAACCTTAAGACTTGCCCTTCTTGTGG AATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAACTGTGCAAAATGGCTGTCAAGACGAG CCTAAGACCAGCACCAAGGCTTCTGGAGGACCCAACTCCAAAACCCAGAATGGACTTCTGAGCCCTCCTG CAGAGGAGAAGCTCACTAACAGCCAGACCTCCCTCTGTGAGATCCTGCAAGAGAAGGGACGGTGGGCAGG GGTGAGCTTGGACCAGTCAGCCCTCCTCCCGCTCAGGTTCAAGAACATCCGTGAGAAAACTGATGCCCAC TTTGTTGATGTCATCAAAGAAGACAGCCTGATGAAAGATTATTTCTTCAAGCCGCCCATCAATCAGTTCA GCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAGGTCATAAAGAA TTCTCCTGTGAAGACGTTCGCCAGTGCCACCTTCAGCTCCCTTCTGGATGTGTTTCTGTCAACCACCGTG TTCTTGATTCTCTCCATCACCTGCTTCCTAAAGTATGGAGCCACCGCCACCCCTCCCCCACCGGCTGCCC TGGCCGTCTTTGGTGCAGACCTGCTGCTGGAGGTGCTTTCCCTCATAGTGTCCATCAGAATGGTGTTTTT CCTAGAGGATGTCATGACATGCACAAAGTGGTTGCTGGAATGGATCGCTGGCTGGCTCCCTCGCCACTGC ATTGGGGCAATCTTGGTGTCTCTTCCTGCCCTGGCTGTCTATTCACACATCACCTCTGAGTTTGAGACCA ACATACATGTTACCATGTTCACTGGCTCTGCGGTGCTGGTGGCCGTTGTGCACTACTGTAACTTCTGCCA GCTCAGCTCCTGGATGAGGTCCTCCCTTGCCACCATCGTGGGGGCTGGGCTGCTGCTTCTGCTCCACATC TCCCTGTGTCAGGACAGTTCCATTGTGATGTCCCCCTTGGACTCAGCACAGAATTTCAGTGCCCAGAGGA ACCCATGCAACAGCTCAGTGCTGCAGGACGGCAGGAGGCCGGCCAGCCTCATAGGCAAGGAGCTTATCCT CACCTTCTTCCTCCTGCTCCTCTTGGTCTGGTTCCTGAACCGGGAGTTCGAGGTCAGCTACCGGCTGCAC TACCATGGGGATGTGGAGGCCGACCTACACCGCACCAAGATCCAGAGCATGAGAGACCAGGCTGACTGGC TACTGCGGAACATCATCCCCTACCATGTGGCTGAGCAGCTCAAGGTCTCTCAGACCTACTCCAAGAACCA TGACAGCGGGGGAGTCATCTTTGCCAGCATTGTCAACTTCAGTGAATTCTATGAGGAGAACTATGAGGGG GGCAAGGAGTGCTACCGTGTCCTCAACGAGCTGATCGGTGACTTCGATGAGCTCTTGAGCAAGCCGGACT ATAATAGCATCGAGAAGATCAAGACCATCGGGGCCACATACATGGCAGCCTCAGGGCTGAACACGGCCCA GTGTCAGGAGGGTGGCCACCCACAGGAGCATCTGCGTATCCTCTTCGAGTTCGCCAAGGAGATGATGCGC GTGGTGGATGACTTCAACAACAATATGTTATGGTTCAACTTCAAGCTCAGGGTCGGCTTTAACCACGGAC CCCTCACAGCAGGTGTCATAGGTACCACCAAGCTGCTGTATGACATCTGGGGGGACACCGTCAACATCGC CAGCAGGATGGACACCACTGGTGTGGAGTGCCGTATCCAGGTGAGCGAAGAGAGCTACCGTGTGCTGAGC AAGATGGGTTATGACTTTGACTACCGAGGGACCGTGAATGTCAAGGGGAAAGGGCAGATGAAGACCTACC TTTACCCAAAGTGCACGGACAATGGAGTGGTTCCCCAGCACCAGCTGTCCATCTCCCCAGACATCCGAGT CCAGGTGGACGGCAGCATTGGGCGGTCTCCCACAGATGAGATTGCCAACTTGGTGCCTTCCGTTCAGTAT TCGGACAAGGCTTCCCTGGGATCTGATGATAGCACACAGGCTAAGGAAGCTCGCCTGTCCTCTAAGAGGT CCTGGAGAGAGCCAGTCAAAGCAGAGGAAAGGTTTCCATTTGGCAAAGCCATAGAAAAGGACAGCTGTGA AGACATAGGAGTAGAAGAGGCCAGTGAACTCAGCAAGCTCAATGTCTCAAAGAGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231592 representing NM_001291910 Red=Cloning site Green=Tags(s) MQLPLYLSLFLGVVYSVLFETFGYHFRNEDCYPSPGPGALHWELLSRALLHVCIHAIGIHLFVMSQVRSR STFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQGDEESENSVKRHATSSPKNRKKKSSIQK APIAFRPFKMQQIEEVSILFADIVGFTKMSANKSAHALVGLLNDLFGRFDRLCEQTKCEKISTLGDCYYC VAGCPEPRADHAYCCIEMGLGMIKAIEQFCQEKKEMVNMRVGVHTGTVLCGILGMRRFKFDVWSNDVNLA NLMEQLGVAGKVHISEATAKYLDDRYEMEDGRVIERLGQSVVADQLKGLKTYLISGQRAKESHCSCAEAL LSGFEVIDDSRESSGPRGQGTASPGSVSDLAQTVKTFDNLKTCPSCGITFAPKSEAGAEGGTVQNGCQDE PKTSTKASGGPNSKTQNGLLSPPAEEKLTNSQTSLCEILQEKGRWAGVSLDQSALLPLRFKNIREKTDAH FVDVIKEDSLMKDYFFKPPINQFSLNFLDQELERSYRTSYQEEVIKNSPVKTFASATFSSLLDVFLSTTV FLILSITCFLKYGATATPPPPAALAVFGADLLLEVLSLIVSIRMVFFLEDVMTCTKWLLEWIAGWLPRHC IGAILVSLPALAVYSHITSEFETNIHVTMFTGSAVLVAVVHYCNFCQLSSWMRSSLATIVGAGLLLLLHI SLCQDSSIVMSPLDSAQNFSAQRNPCNSSVLQDGRRPASLIGKELILTFFLLLLLVWFLNREFEVSYRLH YHGDVEADLHRTKIQSMRDQADWLLRNIIPYHVAEQLKVSQTYSKNHDSGGVIFASIVNFSEFYEENYEG GKECYRVLNELIGDFDELLSKPDYNSIEKIKTIGATYMAASGLNTAQCQEGGHPQEHLRILFEFAKEMMR VVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDTTGVECRIQVSEESYRVLS KMGYDFDYRGTVNVKGKGQMKTYLYPKCTDNGVVPQHQLSISPDIRVQVDGSIGRSPTDEIANLVPSVQY SDKASLGSDDSTQAKEARLSSKRSWREPVKAEERFPFGKAIEKDSCEDIGVEEASELSKLNVSKSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291910
ORF Size	3348 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291910.1, NP_001278839.1
RefSeq Size	7632 bp
RefSeq ORF	3351 bp
Locus ID	11515
MW	124.8 kDa
Gene Summary	Adenylyl cyclase that catalyzes the formation of the signaling molecule cAMP in response to activation of G protein-coupled receptors. Contributes to signaling cascades activated by CRH (corticotropin-releasing factor), corticosteroids and by beta-adrenergic receptors.[UniProtKB/Swiss-Prot Function]

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