Nckap1 (NM_001290745) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C79304; H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1; p125Nap1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231612 representing NM_001290745 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGCTCCGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG GCGTCGGCATGCTCACGCGCCTCTACAACATCAAGAAGCAAGGACAAGTTTGGAAGGCATGTGGCGACCC CAAGGCTAAACCCTCCTACCTTATTGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTT CCTGCTGTAGAAACGCGCAACAACAATCAACAGCTGGCACAGCTACAGAAAGAAAAATCAGAGATTCTGA AAAATCTGGCGTTGTATTACTTTACATTTGTAGATGTTATGGAATTTAAGGACCATGTCTGTGACTTGCT GAATACTATTGACGTTTGCCAAGTCTTCTTTGATATTACTGTGAACTTTGATTTAACAAAGAACTACTTA GACTTGACTGTAACCTATACAACATTAATGATACTGCTGTCCCGGATTGAAGAACGGAAGGCAATCATTG GACTATACAATTATGCACATGAAATGACCCATGGAGCAAGTGACCGAGAATATCCACGCCTTGGTCAGAT GATTGTGGACTATGAAAACCCTTTAAAAAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCA GATGCGCTAATTTCTCTTCAGATGGTGTATCCTAGACGGAACCTTTCAGCAGACCAGTGGAGGAATGCCC AGTTGCTGAGTCTCATCAGTGCACCCAGCACAATGCTTAATCCTGCGCAGTCGGACACTATGCCTTGTGA ATATCTCTCCTTGGATGCAATGGAGAAATGGATCATCTTTGGCTTTATTCTGTGCCATGGGATGCTAAAT ACGGAGGCTACCGCACTGAACCTTTGGAAGCTAGCTCTTCAGAGTAGCTCTTGCCTCTCTCTCTTTCGGG ATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATTCGGGGCTACAATAAACGTAT TAACGACATAAGAGAATGCAAGGAGGCGGCTGTATCCCACGCCGGCTCAATGCACAGAGAAAGACGCAAG TTTTTACGGTCTGCACTAAAAGAACTGGCCACTGTCCTCTCTGACCAGCCTGGCCTGCTTGGTCCCAAGG CACTTTTTGTTTTCATGGCGTTATCTTTTGCCCGTGATGAAATTATCTGGCTGCTCCGTCACGCGGACAA CATGCCAAAGAAGAGTGCAGATGACTTTATAGATAAGCACATTGCTGAGCTAATATTTTACATGGAAGAG CTTAGAGCACATGTCAGGAAGTATGGGCCTGTCATGCAGAGGTACTACGTGCAATACCTCTCTGGCTTTG ATGCTGTTGTCCTCAATGAACTTGTGCAGAACCTGTCTGTTTGCCCTGAGGATGAATCGATCATCATGTC CTCTTTTGTGAACACCATGACCTCCCTAAGTGTGAAGCAAGTTGAAGATGGAGAAGTGTTTGATTTCAGA GGGATGAGATTAGATTGGTTTAGGTTACAGGCGTACACTAGCGTCTCCAAAGCTTCACTTAGCCTTGCAG ATCACAGAGAACTTGGAAAGATGATGAACACAATCATATTCCATACAAAAATGGTAGATTCCTTGGTTGA AATGTTGGTGGAAACATCCGATCTCTCCATATTTTGTTTTTACAGCCGTGCTTTTGAGAAGATGTTTCAG CAGTGCTTGGAGCTGCCCTCTCAGTCAAGATACTCCATTGCCTTTCCTCTGCTCTGCACTCACTTCATGA GCTGCACACATGAACTGTGTCCTGAAGAGCGCCATCATATAGGAGATCGTAGCCTTTCCCTGTGCAATAT GTTCCTGGATGAGATGGCCAAACAAGCTCGAAATCTCATCACCGATATTTGCACAGAACAGTGTACTCTT AGTGACCAGTTATTGCCGAAGCATTGTGCCAAAACCATCAGTCAAGCAGTGAATAAGAAGTCAAAAAAAC AGACGGGCAAGAAAGGGGAACCTGAAAGGGAAAAACCGGGTGTGGAGAGCATGAGGAAAAACAGGCTGGT AGTGACCAACCTTGATAAGTTGCACACTGCACTTTCGGAGTTATGCTTCTCCATAAATTATGTTCCAAAC ATGGCGGTGTGGGAGCACACCTTCACGCCGAGGGAGTATTTGACTTCTCATCTGGAAATCCGGTTCACTA AATCAATTGTTGGAATGACTATGTATAATCAAGCTACACAGGAAATTGCAAAGCCATCAGAGCTTCTAAC AAGCGTAAGAGCATATATGACTGTACTCCAGTCCATAGAGAACTATGTGCAGATTGATATCACCAGAGTA TTTAATAATGTCCTTCTTCAGCAAACACAACACTTAGACAGCCATGGAGAACCAACCATCACGAGTCTGT ATACAAACTGGTACTTGGAAACTTTATTAAGACAAGTCAGCAATGGCCATATAGCTTATTTTCCTGCAAT GAAAGCATTTGTGAATTTACCCACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGATATATCA GAAATGAGATCATTATCAGAGCTCCTAGGCCCATATGGGATGAAGTTCCTGAGTGAGAGCCTTATGTGGC ACATTTCATCACAAGTAGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTTCTAACACAAATGAG GACCAGCTTTGACAAACCAGACCAGATGGCTGCACTCTTTAAAAGATTATCATCTGTTGACAGTGTCTTG AAAAGGATGACGATAATTGGTGTTATTTTATCCTTCCGGTCATTGGCACAAGAAGCATTGAGAGATGTCC TGTCCTACCACATTCCTTTCCTCGTGAGCTCCATTGAAGATTTCAAGGATCACATTCCTCGGGAAACTGA TATGAAGGTTGCAATGAATGTGTATGAGCTGTCCTCGGCTGCTGGGTTACCATGTGAGATTGATCCTGCC TTAGTCGTAGCTCTTTCCTCACAAAAATCAGAGAACATTAGTCCAGAGGAAGAATACAAGATCGCTTGTC TCCTGATGGTCTTTGTAGCAGTTTCCTTGCCTACACTGGCCAGCAATGTAATGTCACAGTACAGCCCTGC AATAGAAGGACACTGCAACAATATACATTGTTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTT ACAATTCACAAAGGAAGTATTGAAGACCGACTTAAGGAATTCCTGGCGCTTGCATCATCCAGTCTACTGA AAATTGGCCAAGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTGCTACTGGATATGAT TGTACAAGAATCACCATTCCTGACAATGGATCTCTTGGAATCTTGTTTTCCTTATGTGTTGCTGAGAAAT GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231612 representing NM_001290745 Red=Cloning site Green=Tags(s) MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKF PAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYL DLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLS DALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLN TEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRK FLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEE LRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFR GMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQ QCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTL SDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPN MAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRV FNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDIS EMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVL KRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPA LVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALF TIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRN AYHAVYKQSVTSSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290745
ORF Size	3402 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290745.1, NP_001277674.1
RefSeq Size	4421 bp
RefSeq ORF	3405 bp
Locus ID	50884
MW	130 kDa
Gene Summary	Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function]

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