Gak (NM_001282051) Mouse Tagged ORF Clone
CAT#: MR231619
- TrueORF®
Gak (myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak), transcript variant 2
"NM_001282051" in other vectors (1)
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CNY 11,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D130045N16Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231619 representing NM_001282051
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAGGCAGAAACCACCCATCATCCACAGAGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGG GGACCATTAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGTGC CCAGAAGCGAGCCATGGTGGAGGAAGAGATCACAAGGAACACCACACCCATGTACAGAACACCAGAAATT GTAGACCTGTATTCCAACTTCCCCATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACC TGCTGTGTTTCCGGCAGCATCCTTTTGAGGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCAT TCCTGTGAATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGCCATGCTAAAGGTCAACCCAGAG GAGAGGCTGTCCATTGCTGAAGTGGTCCGACAACTGCAGGAAATTGCAGCAGCTAGGAATGTGAACCCCA AAGCTCCCATCACAGAGCTGCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACC ACCTTGCGGGGGCACCGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTACGGTGGG TTTCTTGATATTCTGCGGGGTGGGACCGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCA TCCAGTCTGTGGCTAACTATGCAAAGGGTGATCTGGACATATCTTACATCACATCCAGGATTGCAGTGAT GTCATTCCCAGCAGAAGGTGTGGAGTCTGCAATCAAAAACAATATAGAGGATGTGCGAATGTTTCTGGAT GCTAAGCATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGGATATACCGGGCTTCCAAGTTTCACA ACCGGGTCACTGAGTGTGGCTGGGCAGTCAGACGGGCACCACATCTCCACAGTTTGTATACTCTATGTAG GAGCATGCATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTGCATTGCATGGATGGGAGAGCT GCATCTGCTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGT ACATGTTCAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATATGTATG TGACATGGTGGCAGAGGAGCCCATCACGCCCCATAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACC CCCGTGCCACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCGTTCTGTGAGGTCTACGTTGGAGAGGAGC GTGTCACCACCACGTCGCAGGAATATGACAGAATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCAT CCCCCTGGGCGTAACAGTTCAAGGAGACGTGCTCATCATCATCTACCATGCCAGGGCCACACTGGGAGGG AGGCTGCAGGCTAAGATGGCGTCCATGAAAATGTTCCAGATCCAGTTCCACACTGGGTTCGTGCCTCGAA ACGCAACCACTGTGAAATTTGCAAAATATGACCTGGATGCTTGTGATATTCAAGAGAAGTACCCAGATCT GTTCCAGGTGAACCTGGAAGTGGAGGTAGAGCCTAGAGACAGGCCCAGCCGAGAAGCTCCACCTTGGGAG AACACCAGCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAGCAGGACATTCTGT CTAAGTTTGGGAAGCCGGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTC TCCGGAGGCTGAGATCACAGAGTCGGACTCACCGCAGAGCAGCAGTACGGACACCAACCACTTTCTTCAC ACACTGGATTGGCAGGAGGAAAAAGAACCAGAGACTGGGTTAGACAATACCTCTCCTAAGGAGAGTCAGT CTGTCCTGATTGCAGACGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATTCCCCAGTGAGGA GAGGAAACCAGGAGCTGGAGAAGATACACCAAGGCTGGCTGCTGGGACCAAACAGCAAGACTTAATATTT GATGTGGGCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCCGAAGAAGGTGTTGATCTCCTGGGGCTAC ACTCTGAGGGGGACTTAAGGCCTGCTGCCCCCTTACAGGCTTGTGGGGTCCCATCTAGCAACACTGACCT GTTGAGCTGCCTTCTTGAACCATCTGATGCTGCTCAAGTAGGGCCTCCTGGTGACCTGCTTGGTGGTGAG GCTCCACTGCTGCTAGCAAGCCCAGTTTCTCCTCTTGGGCTGCAGAACAACCTACAAGGAAAAGTCCCTG ACACTGTGGACCCATTTGACCAGTTCCTGCTGTCATCCAACTCAGACACCCAGCCCTGCTCCAAGCCTGA TCTCTTTGGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCCACCCACAGTGCC CCACCCCCATCCTGTAGCACTGCCTTCCTGCACCTAGGGGATCTGCCAGCAGAGCCCAGCAAGGTAATAG CTTCATCCAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGACACAGCTACACCTGGGCCAGC CTCCATTCCAGTACCAGAAGGTACCCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCTGGCCCCAACCCC AGCCAAACCAAGTCTCAGAACCTTGACCCATTTGCTGATCTCAGTGATCTCAGCTCTAGCCTCCAAGGCC TGCCTGCTGGACTTCCTGCAGGGGGCTTTGTTGGCGCACCAGCCCCCACTCAAAAGAGCAACAGCCCCTG GCAGGCAAATCGTCCCACAGCCCCTGGAACCTCATGGACCCCCCAGGCCAAGCCAGCCCCCAGAGCCTCT GAACAGCTAAGGTCTCACTTCAGTGTGATCGGGGCCCGAGAAGAGAGGGGCGTCCGTGTGCCCAGCTTTG CCCAAAAGCCAAAGGTCTCAGAAAATGATTTTGAAGATCTTCTGCCTAATCAAGGCTTCTCTAAGTCTGA CAAGAAGGGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCACTCAAA TTGAAGCTTTTGGACTGGATTGAAGGCAAGGAGAGGAACATTCGTGCACTGCTGTCCACTCTGCACACGG TATTGTGGGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAA GAAGCAGTACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCGTATGAACAGTAT GCCAAGATGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCGAGGCCCCTCT TC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231619 representing NM_001282051
Red=Cloning site Green=Tags(s) MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEI VDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPE ERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGG FLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLD AKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRA ASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMT PVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGG RLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWE NTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLH TLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIF DVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGE APLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSA PPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNP SQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRAS EQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLK LKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQY AKMIFMELNDAWSEFENQGSRPLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282051 |
ORF Size | 3432 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282051.1, NP_001268980.1 |
RefSeq Size | 4793 bp |
RefSeq ORF | 3435 bp |
Locus ID | 231580 |
UniProt ID | Q99KY4 |
MW | 126.5 kDa |
Gene Summary | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229408 | Gak (untagged) - Mouse cyclin G associated kinase (Gak), transcript variant 2 |
CNY 10,930.00 |