Hipk2 (NM_001294144) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110014O20Rik; B230339E18Rik; Stank
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231646 representing NM_001294144 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTG CCTTCTGTAGTGTGAAGAAACTAAAAGTAGAGCCAAGTTCCAACTGGGACATGACTGGGTACGGCTCCCA CAGCAAAGTGTACAGCCAGAGCAAGAACATACCACCTTCTCAGCCAGCCTCCACAACCGTCAGCACCTCC TTGCCAATCCCCAACCCAAGCCTACCTTACGAGCAGACCATCATCTTCCCAGGAAGTACTGGACACATAG TTGTAACATCAGCAAGTAGCACTTCTGTCACCGGGCAAGTCCTCGGTGGACCACATAACCTAATGCGTCG AAGCACTGTGAGCCTCCTTGACACCTACCAAAAATGTGGACTCAAGCGCAAGAGTGAGGAGATTGAGAAC ACAAGCAGCGTGCAGATCATTGAAGAGCATCCACCCATGATTCAGAATAATGCCAGCGGGGCCACTGTAG CCACTGCCACCACATCCACTGCCACCTCCAAAAACAGTGGCTCCAACAGTGAAGGGGACTATCAGCTGGT GCAGCATGAAGTACTGTGCTCCATGACCAACACCTATGAGGTTTTAGAGTTCTTGGGGCGAGGGACATTT GGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAAATTGTGGCCATTAAGATCCTCAAGAACCACC CCTCCTATGCCCGGCAAGGCCAGATTGAAGTGAGCATCTTGGCCCGGCTGAGCACGGAGAGCGCTGATGA CTACAATTTTGTGCGGGCCTATGAGTGCTTCCAGCACAAGAACCACACGTGCTTAGTCTTTGAGATGTTG GAGCAGAACCTTTATGACTTTCTGAAACAGAACAAGTTTAGTCCCTTGCCCCTCAAGTATATTCGCCCAG TCCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTGGGTCTTATCCATGCTGACCTCAAACC AGAAAACATCATGCTGGTGGATCCATCCAGACAACCGTACCGAGTGAAGGTCATTGACTTTGGTTCAGCT AGTCATGTGTCCAAAGCTGTCTGCTCTACGTACTTGCAATCCAGATACTACCGGGCCCCTGAGATCATCC TTGGTTTGCCATTCTGTGAGGCAATTGACATGTGGTCCCTTGGTTGTGTCATTGCTGAACTGTTCCTGGG CTGGCCATTATACCCAGGTGCTTCTGAGTATGATCAGATTCGGTATATTTCACAAACACAGGGTTTACCA GCTGAATATTTATTAAGTGCAGGGACAAAGACAACTAGGTTTTTCAACCGTGACACAGACTCACCGTATC CTTTGTGGAGGCTAAAGACACCAGATGACCATGAAGCAGAGACGGGAATTAAGTCAAAGGAAGCAAGAAA GTACATTTTCAACTGTTTGGATGATATGGCCCAGGTGAACATGACAACAGATTTGGAAGGGAGTGACATG TTAGTAGAGAAGGCAGACCGACGGGAGTTCATTGACCTGTTAAAGAAGATGCTGACCATCGATGCTGATA AGAGAGTCACTCCCATTGAAACTCTGAACCACCCCTTTGTCACCATGACACACCTGCTTGACTTCCCCCA TAGTGCCCATGTCAAATCTTGTTTCCAAAACATGGAGATTTGCAAGCGCCGGGTGAATATGTATGACACA GTGAACCAGAGCAAAACACCTTTCATCACTCACGTGGCCCCCAGCACATCCACCAACTTGACCATGACCT TTAACAACCAGCTGACCACTGTCCACAACCAGCCCTCAGCGGCATCCATGGCCGCGGTGGCCCCGCGGAG CATGCCCCTGCAGACGGGAACAGCCCAAATTTGTGCCCGACCTGATCCCTTCCAGCAAGCTCTCATCGTG TGTCCCCCTGGCTTCCAAGGCCTGCAGGCCTCTCCCTCCAAGCACGCTGGCTACTCAGTGCGGATGGAAA ATGCTGTCCCCATCGTCACCCAGGCGCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGCCTGCTTGCTCA GGCCTGGCCAGGTGGGGCCCAACAGATCCTACTTCCTCCAGCATGGCAGCAGCTGACTGGCGTGGCCACC CACACATCTGTACAGCATGCAGCTGTGATTCCTGAGACCATGGCAGGCACTCAGCAACTGGCTGACTGGA GGAACACGCATGCTCATGGAAGTCATTACAATCCCATCATGCAGCAGCCTGCACTCTTGACTGGTCATGT GACCCTTCCAGCTGCCCAGCCCTTAAATGTGGGTGTAGCCCATGTGATGAGGCAACAACCAACCAGCACC ACCTCCTCCAGGAAGAGTAAGCAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGACCTCTT CACAGGCTATCAGCTCCCCTCAGCGATCCAAGCGTGTCAAGGAGAACACTCCCCCACGGTGCGCCATGGT ACACAGCAGCCCAGCTTGCAGCACCTCAGTCACCTGTGGGTGGGGCGACGTGGCCTCCAGCACCACCCGG GAGCGACAGCGGCAGACGATTGTCATCCCCGACACCCCGAGCCCCACAGTCAGTGTCATCACCATCAGCA GTGACACCGATGAAGAAGAGGAGCAGAAGCACGCCCCCACCAGCACAGTCTCCAAGCAAAGAAAAAATGT CATCAGCTGTGTCACCGTCCACGACTCTCCCTACTCTGACTCCTCCAGCAACACCAGCCCCTACTCGGTG CAGCAGCGCACAGGGCACAACGGCACCAACACCTTGGACACCAAGGGGGGCCTAGAGAATCACTGCACTG GCAACCCCCGCACCATCATCGTACCCCCACTGAAGACCCAGGCCAGTGAAGTGTTGGTAGAATGTGACAG CCTAGGGCCAGCGATCAGTGCCAGTCACCATTCGTCCTCCTTCAAGTCCAAGTCCTCCAGCACCGTGACC TCCACCAGTGGACACTCTTCAGGGAGCTCTTCAGGAGCCATCGCCTACCGTCAGCAGCGGCCAGGCCCCC ACTTCCAGCAGCAGCAGCCCCTCAATCTCAGCCAGGCCCAGCAGCACATGGCTGCGGACCGCACCGGGAG TCACCGTCGACAGCAGGCCTACATCACTCCGACCATGGCGCAAGCTCCGTACACCTTCCCGCACAACAGC CCCAGCCATGGCACTGTTCACCCCCACCTGGCTGCGGCTGCCCACCTCCCCACCCAGCCTCACCTCTACA CCTATACAGCGCCCACAGCCCTAGGCTCCACCGGCACTGTAGCCCACCTGGTGGCATCCCAAGGCTCAGC ACGCCACACCGTGCAGCACACTGCCTATCCGGCCAGCATCGTCCACCAGGTCCCTGTGAGCATGGGGCCC CGGGTCCTGCCCTCGCCCACCATCCACCCCAGTCAGTATCCAGCCCAGTTTGCCCACCAGACCTATATCA GCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGTCCTGCCAAGGTCAACCAATACCCTTA CATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231646 representing NM_001294144 Red=Cloning site Green=Tags(s) MAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKVEPSSNWDMTGYGSHSKVYSQSKNIPPSQPASTTVSTS LPIPNPSLPYEQTIIFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIEN TSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTF GQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEML EQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSA SHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLP AEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDM LVEKADRREFIDLLKKMLTIDADKRVTPIETLNHPFVTMTHLLDFPHSAHVKSCFQNMEICKRRVNMYDT VNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQPSAASMAAVAPRSMPLQTGTAQICARPDPFQQALIV CPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQAWPGGAQQILLPPAWQQLTGVAT HTSVQHAAVIPETMAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTST TSSRKSKQHQSSVRNVSTCEVTSSQAISSPQRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTTR ERQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSV QQRTGHNGTNTLDTKGGLENHCTGNPRTIIVPPLKTQASEVLVECDSLGPAISASHHSSSFKSKSSSTVT STSGHSSGSSSGAIAYRQQRPGPHFQQQQPLNLSQAQQHMAADRTGSHRRQQAYITPTMAQAPYTFPHNS PSHGTVHPHLAAAAHLPTQPHLYTYTAPTALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMGP RVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAKVNQYPYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001294144
ORF Size	3504 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001294144.1, NP_001281073.1
RefSeq Size	4175 bp
RefSeq ORF	3507 bp
Locus ID	15258
UniProt ID	Q9QZR5
MW	128 kDa
Gene Summary	Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.[UniProtKB/Swiss-Prot Function]

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