Nup98 (NM_001287165) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4732457F17; AI849286; Nup96
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231648 representing NM_001287165 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAACAAATCATTTGGAACCCCCTTTGGGGGTAGTACAGGGGGCTTTGGCACAACGTCAACATTTG GGCAAAATACTGGCTTTGGTACGACTAGTGGAGGAGCATTTGGAACATCTGCATTTGGTTCTAGCAACAA TACTGGAGGCTTATTTGGAAATTCACAGACCAAACCAGGAGGATTATTTGGTACCAGTTCATTTAGCCAG CCAGCAACCTCCACAAGCACTGGGTTTGGGTTTGGCACATCAACAGGAACATCAAATAGCTTATTTGGAA CTGCAAGTACCGGGACCAGTCTTTTCTCATCCCAGAACAATGCATTTGCACAAAATAAACCAACTGGCTT TGGGAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACTACAAATACCACCTCTAATCCTTTT GGTAGCACATCTGGCTCCCTTTTTGGGCCAAGTAGTTTTACAGCAGCACCTACAGGAACTACCATCAAAT TTAATCCTCCCACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATCAGTACAAAGCATCA GTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCATTAGAGGAACTACGTTTGGAGGATTATCAGGCT AACCGGAAAGGCCCACAGAACCAAGTGGGAGGAGGCACCACGGCTGGCTTATTTGGGTCTTCTCCAGCAA CTTCCAGTGCAACAGGGCTCTTCAGCTCCTCCACCACTAATTCAGCCTTTTCATATGGTCAGAACAAAAC TGCCTTTGGAACTAGCACAACTGGATTTGGAACAAATCCAGGTGGTCTCTTTGGCCAACAGAATCAACAG ACTACCAGTCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCCGAATACTGGCTTTTCCTTTGGTA ATACCAGCACCCTTGGACAGCCAAGCACCAATACTATGGGCCTATTTGGAGTAACCCAAGCCTCACAACC AGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGGACAGCATTTGGGACAGGAACAGGTCTCTTT GGGCAGCCCAATACTGGATTTGGTGCAGTTGGTTCGACCCTGTTTGGCAATAACAAGCTTACAACTTTTG GAACCAGCACAACCAGTGCTCCTTCATTTGGTACAACCAGTGGCGGGCTCTTCGGGTTTGGCACAAATAA CAGTGGGAGCAGTATTTTTGGAAGTAAGCCAGCAGCTGGAACTTTGGGAACTGGACTTGGTACAGGATTT GGAACAGCTCTTGGTGCTGGACAGGCATCTTTGTTTGGAAACAACCAACCTAAGATTGGAGGGCCTCTTG GTACAGGAGCCTTTGGGGCCCCTGGATTTAATACTTCGACAGCCATTTTGGGCTTTGGCGCCCCCCAGGC CCCAGTAGCTTTGACAGATCCAAATGCTTCTGCTGCCCAGCAGGCTGTTCTCCAGCAGCACCTCAATAGC CTAACATACTCACCCTTTGGAGACTCCCCCCTCTTCCGGAATCCTATGTCAGATCCTAAGAAGAAAGAAG AGAGACTGAAACCAACCAATCCAGCTGCTCAGAAAGCTCTTACAACACCTACTCATTATAAACTTACACC TCGCCCTGCTACCAGAGTCAGGCCAAAGGCTTTGCAAACAACAGGCACAGCCAAATCACATCTCTTTGAT GGGCTGGATGACGATGAACCATCTCTAGCCAACGGAGCATTCATGCCTAAAAAGAGCATCAAGAAGTTGG TTTTGAAAAATCTCAACAATAGCAATCTCTTTTCTCCTGTTAATCATGATTCAGAAGATCTAGCTTCACC CTCTGAGTATCCAGAAAATGGAGAAAGATTTAGCTTCCTGAGCAAACCTGTTGATGAGAACAATCAGCAG GATGGAGAAGATGACTCTCTTGTATCACGATTTTACACTAATCCTATTGCCAAACCCATTCCACAAACTC CAGAGAGTGTTGGAAACAAAAATAACAGTAGCAGCAATGTGGAAGATACCATTGTTGCCTTGAACATGCG TGCTGCTTTGCGCAATGGATTGGAAGGAAGCAGTGAAGAGACGTCATTCCATGATGAGTCATTGCAAGAT GACCGAGAAGAGATAGAAAATAATGCTTACCACATACACCCAGCAGGCATTGTTCTCACAAAAGTTGGTT ATTACACTATCCCATCTATGGATGACCTTGCTAAAATTACCAACGAGAAGGGAGAATGCATTGTTTCTGA CTTCACCATTGGTCGTAAAGGATATGGCTCAATCTATTTTGAAGGAGATGTGAATTTGACAAATCTAAAT TTGGATGATATTGTGCATATCCGAAGGAAAGAAGTTATTGTCTATGTAGATGATAACCAAAAGCCACCTG TGGGTGAAGGGCTAAATAGGAAGGCTGAAGTTACTTTGGATGGAGTTTGGCCAACAGATAAAACATCCCG GTGTTTAATAAAGAGTCCAGATCGACTTGCTGATATCAACTATGAGGGGAGATTAGAAGCAGTCTCAAGA AAGCAAGGGGCCCAATTCAAGGAGTATCGGCCTGAAACTGGTTCTTGGGTATTTAAGGTCTCCCATTTTT CCAAGTATGGCCTTCAGGATTCTGATGAAGAGGAGGAGGAACACCCACCCAAAACGACTTCAAAGAAGCT GAAGACTGCCCCTTTGCCCCCTGCAGGCCAGGCAACCACTTTCCAGATGACTCTTAATGGCAAGCCTGCA CCCCCACCTCAGAGCCAGAGCCCAGAAGTGGAGCAGTTAGGCAGGGTTGTGGAACTGGACAGCGACATGG TAGATATCACCCAGGAGCCAGTTCCAGATTCTGTGTTAGAAGAGAGTGTGCCCGAGGATCAGGAGCCTGT GTCTGCTTCAACGCATATTGCATCTTCACTGGGAATTAATCCACATGTTTTACAGATCATGAAAGCATCA TTGCTTGTTGACGAAGAAGATGTAGATGCCATGGATCAACGCTTTGGTCACATCCCTTCCAAAGGAGAGA CTGTCCAGGAAATCTGTTCTCCTAGACTCCCCATTTCAGCCTCCCACTCATCAAAATCCCGCTCCATAGT TGGTGGGTTGCTGCAATCAAAATTTGCAAGTGGAACTTTTCTTTCACCAAGTGCCTCCGTGCAAGAATGT CGCACTCCCAGGACATCATCTCGCATGAACATCCCATCCACATCCCCCTGGTCTGTCCCTCTGCCCCTGG CCACTGTGTTCACAGTGCCCAGCCCAGCCCCTGAGGTTCAGCTAAAAACAGTGGGGATACGAAGGCAACC AGGCCTAGTCCCTCTTGAAAAATCCATTACATATGGCAAGGGGAAGCTCTTGATGGACATGGCCCTATTC ATGGGACGTTCATTTCGGGTTGGTTGGGGTCCCAACTGGACTCTTGCTAATAGTGGAGAACAACTGCATG GCTCCCATGAACTGGAAAATCATCAGGTTGCCGATTCTATGGAATATGGATTCCTGCCCAATCCAGTAGC TGTTAAATCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231648 representing NM_001287165 Red=Cloning site Green=Tags(s) MFNKSFGTPFGGSTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQ PATSTSTGFGFGTSTGTSNSLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPF GSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQA NRKGPQNQVGGGTTAGLFGSSPATSSATGLFSSSTTNSAFSYGQNKTAFGTSTTGFGTNPGGLFGQQNQQ TTSLFSKPFGQATTTPNTGFSFGNTSTLGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLF GQPNTGFGAVGSTLFGNNKLTTFGTSTTSAPSFGTTSGGLFGFGTNNSGSSIFGSKPAAGTLGTGLGTGF GTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTSTAILGFGAPQAPVALTDPNASAAQQAVLQQHLNS LTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFD GLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNHDSEDLASPSEYPENGERFSFLSKPVDENNQQ DGEDDSLVSRFYTNPIAKPIPQTPESVGNKNNSSSNVEDTIVALNMRAALRNGLEGSSEETSFHDESLQD DREEIENNAYHIHPAGIVLTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLN LDDIVHIRRKEVIVYVDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSR KQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPPKTTSKKLKTAPLPPAGQATTFQMTLNGKPA PPPQSQSPEVEQLGRVVELDSDMVDITQEPVPDSVLEESVPEDQEPVSASTHIASSLGINPHVLQIMKAS LLVDEEDVDAMDQRFGHIPSKGETVQEICSPRLPISASHSSKSRSIVGGLLQSKFASGTFLSPSASVQEC RTPRTSSRMNIPSTSPWSVPLPLATVFTVPSPAPEVQLKTVGIRRQPGLVPLEKSITYGKGKLLMDMALF MGRSFRVGWGPNWTLANSGEQLHGSHELENHQVADSMEYGFLPNPVAVKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287165
ORF Size	3510 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287165.1, NP_001274094.1
RefSeq Size	4086 bp
RefSeq ORF	3513 bp
Locus ID	269966
UniProt ID	Q6PFD9
MW	123.5 kDa
Gene Summary	Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).[UniProtKB/Swiss-Prot Function]

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