Anks1 (NM_001286040) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Anks1a; mKIAA0229
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231667 representing NM_001286040 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGAGCAGGAGCTGCTGGAGGCGGCCCGCACCGGGCACCTCCCGGCCGTGGAGAAGCTGCTGT CCGGGAAGCGGCTCTCCTCCGGCTTCGGCGGCGGCGGCGGCGGCTCAGGCAGCGGCGGCGGCTCCGGGGG CGGCGGCCTCGGTTCTTCTAGCCACCCGCTCTCCAGTCTGCTGAGCATGTGGAGAGGGCCGAACGTGAAC TGTGTAGATAGCACCGGCTACACGCCACTACACCACGCTGCTTTGAATGGACACAGGGATGTGGTCGAGG TCCTTCTGAGGAACGACGCGCTGACCAACGTTGCTGACTCGAAAGGCTGCTACCCGCTGCACTTGGCAGC GTGGAAAGGAGACGCCCAGATAGTACGTCTGCTCATCCAGCAAGGGCCCTCACACACCAGAGTCAACGAA CAGAATGCTCTTGAGATCAGAGAACTCAAAAAGTACGGCCCCTTTGACCCTTATATCAATGCCAAGAACA ACGACAATGAGACGGCCCTGCACTGCGCGGCCCAGTACGGCCACACGGAGGTCGTGAAGGCTCTGCTGGA GGAGCTCACGGACCCCACCATGCGCAACAACAAGTTTGAAACGCCCCTGGACTTGGCGGCGCTGTACGGG CGGCTGGAGGTGGTGAAGCTGCTGCTGGGTGCACACCCCAATCTCCTGAGCTGCAGCACTCGGAAACACA CGCCCCTGCACTTGGCAGCAAGGAATGGCCACAAGGCCGTGGTGCAGGTCCTGCTGGACGCCGGCATGGA TAGCAACTATCAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTG CAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTG TCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAG AAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCA CCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACG AGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACG ATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCT AGGGAACGTCCGCCGCCTCCAGCAAAACCGCCACCCGATGAAGAGGAGGAAGAACGCGTAGATAAGAAGT ATTTTCCCTTGGCAGCCTCTGAGGGCCTGGCTGTGAGACCCAGGATTCAGAGTAGTGCTCCCCAGGAAGA GGAGGAGCACCCATATGAGCTGCTGCTCACGGCAGAAACCAAGAAGCTGGGGACCACAGACGGGAGGACT GAAGACCACAGGCAGAGCGGCAGCGGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAGGTCCCAG AGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCCAGCTGCT CACGGCCCCCAGCCAGAGCCACCCGGAGTCCTCCCAGCAGGACGCCTGCCACGAGGCCAGCATGCAGCTA GAGGAGCCAGGCGTGCAAGGCACTGAACCCCCGCAGCCCGGCGTCCCTGACCAAAGCAAGAGAGTGGGCT TGCCGGCAGGCCTGACAGCCCTGGCCAGCAGAACATACCTCGATGCATTGACTCACACAGTACCTCTGCG CCCTGCTGGTGCCGAGGAAGAAGACCAGAGCGGGCCCAGAAGCAGAGCCCCTCCCACCAGCAAGCCCAAA GCTGAGCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCCACGGAGG ACGCCACAATGGGGAGTCGCAGCGAGTCCTTGTCCAACTGCAGCATCGGGAAGAAGAGGTTGGAGAAATC ACCTTCCTTTGCCTCAGAGTGGGATGAGATTGAGAAAATCATGAGCTCCATTGGGGAAGGGATTGACTTC TCTCAGGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTGGAGTCGA TTGGCCTGCAGCAGTACGAGAGCAAGCTGCTGCTGAACGGCTTTGATGATGTCCGCTTCCTGGGGTCTAA TGTGATGGAAGAGCAGGACCTGAGGGAGATTGGCATCAGTGACCCACAGCACCGGCGGAAGCTGCTGCAG GCTGCACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGACGGGGTCAGCCCCACCTCAGTGCCCTCAT GGCTGGACTCGCTGGGGCTGCAGGACTATGTCCACTCTTTCCTGTCCAGTGGCTACAGCTCCATCGACAC TGTGAAGAACCTCTGGGAGCTAGAGCTTGTCAATGTCCTGAAAGTTCACCTGCTGGGCCACCGCAAGCGC ATTATCGCCTCTCTAGCAGACAGGCCCTATGAGGAGCCACCCCAGAAGCCTCCAAGGTTTTCCCAGCTAA GATGCCAGGATTTAATCTCCCAGACATCGTCCCCGCTGAGCCAGAATGATTCCTGCACAGGGCGGTCGGC AGACCTGCTGCTGCCCTCAGCAGACACAAGCAGGAGGCGGCACGACAGCCTGCCCGACCCTGGGACCGCC TCCCGAGCAGACCGCTTCCGAGTCCAGGAGGAGCCCAGTGAGACCAAGCTGACCCTCCGTCCACCCAGCC TAGCCGCGCCCTATGCTCCTGTTCAGAGCTGGCAGCACCAGCCAGAAAAGCTCATCTTTGAGTCCTGCGG TTATGAGGCCAATTATCTGGGTTCCATGCTGATCAAAGATCTGCGAGGGACAGAGTCCACCCAAGATGCC TGTGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACA AAGGTGTCAAGTTCATCGATGCTTCCAACAAGAACGTCATTGCGGAGCACGAGATCCGGAACATTTCCTG CGCCGCGCAGGACCCAGAGGACCTGTGTACCTTCGCGTACATCACCAAGGACCTGCAGACGAGCCACCAC TACTGCCACGTTTTCAGCACAGTGGATGTGAACCTGACCTACGAGATCATCCTGACCCTGGGGCAGGCAT TTGAGGTGGCCTATCAGCTGGCCCTCCAAGCCCAGAAATCCAGGACCATGGCGGCCTCTGCAGCTTCGAT GATTGAGACAAAATCCTCCAAACCAGTGCCTAAGCCTCGGGTCGGCATGAGGAAGTCTGCAGTGCCGGTG CCCCCCGACTCTCGCTGTTGTCACTGTCACACCTGCACCACTCACCGTCCTTCCTACCTACCGCTGCCGT CCGTTAGTCCTGGAGTCAAGCTGGAACCACCTGATTCTGACCAAGAGGCCCCGTCCCACGCTAGTGTTTC CTGGATCGTGGACCCAAAGCCAGACTCGAAGCGGAGCCTCAGCACCAAGTACGAGACCACTATCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231667 representing NM_001286040 Red=Cloning site Green=Tags(s) MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGSGSGGGSGGGGLGSSSHPLSSLLSMWRGPNVN CVDSTGYTPLHHAALNGHRDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIQQGPSHTRVNE QNALEIRELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYG RLEVVKLLLGAHPNLLSCSTRKHTPLHLAARNGHKAVVQVLLDAGMDSNYQTEMGSALHEAALFGKTDVV QILLAAGIDVNIKDNRGLTALDTVRDLPSQKSQQIAALIEDHMTGKRSVKEVDRTSTAQLPLLSNTDAIA PMSQGSMEKTVTELILHFDTHADEEGPYEALYNAVSCHSLDSTASGRSSDRDSMNKEAEATGTRAAGVRP RERPPPPAKPPPDEEEEERVDKKYFPLAASEGLAVRPRIQSSAPQEEEEHPYELLLTAETKKLGTTDGRT EDHRQSGSGRSQDSVEGQDGQVPEQFSGLLHGSSPVCEVGQDPFQLLTAPSQSHPESSQQDACHEASMQL EEPGVQGTEPPQPGVPDQSKRVGLPAGLTALASRTYLDALTHTVPLRPAGAEEEDQSGPRSRAPPTSKPK AELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDF SQEQQKISGSRTLEQSVGEWLESIGLQQYESKLLLNGFDDVRFLGSNVMEEQDLREIGISDPQHRRKLLQ AARSLPKVKALGYDGVSPTSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKR IIASLADRPYEEPPQKPPRFSQLRCQDLISQTSSPLSQNDSCTGRSADLLLPSADTSRRRHDSLPDPGTA SRADRFRVQEEPSETKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDA CAKMRKSTEHMKKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCTFAYITKDLQTSHH YCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRTMAASAASMIETKSSKPVPKPRVGMRKSAVPV PPDSRCCHCHTCTTHRPSYLPLPSVSPGVKLEPPDSDQEAPSHASVSWIVDPKPDSKRSLSTKYETTIF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286040
ORF Size	3567 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286040.1, NP_001272969.1
RefSeq Size	7092 bp
RefSeq ORF	3570 bp
Locus ID	224650
MW	130 kDa
Gene Summary	Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction.[UniProtKB/Swiss-Prot Function]

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