Aff3 (NM_001290814) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3222402O04Rik; A730046J16; LAF-4; Laf4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231699 representing NM_001290814 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGAGTCGCTGTGTGTCTATGAACCGGACA GGAATGCGTTACGGAGGAAAGAGCGAGAAAGAAGAAGTCAAGAAACACAGCAGGACAGTGGCAGCTTTAA CTCCGGTTACTCGCTCTTCAGCGAACCCTACAAGACGAACAAGGGGGATGAACTCTCCAACCGGATCCAG AATACATTAGGAAATTATGATGAGATGAAAGACTTTTTAACTGATAGATCCAACCAGAGTCATCTTGTTG GCGTCCCCAAGCCAGGTGTCCCTCAGACTCCCGTGAACAAGATAGATGAGCATTTTGGGGCAGAGTCGAG AGCCCAGCCCCAGCCCTCAACCGTCTGCAGCACTGCGTCCTCTACACCTGCAGCTGTCCCTGTGCAGCAG GGCAAGAGAGGTGCCATGGGCTGGCAGAAGGCTGGGCACCCACCGTCCGATGGCCAACAGAGAGCAGCGC AACAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTGTTGGCAGACAGCAGACAAGGGCCAAGCAAGTGTG CAATATGGAGACGGGGCTTCAGACCCAGGAGAGGCCACCTGCAATGGCAGCCAAGCACGGCGGCAGTGGA CACTGTGTTCAGAACTTTCCCCCATCCCTGGCTTCAAAACCCAGCCTGGTGCAGCAGAAACCAACTGCCT ACGTGCGGCCAATGGATGGCCAGGACCAAGCTCCTGACGAATCTCCTAAGCTGAAGTCATCCACAGAAAC TGCTGTGCACTGCACAGCCTACAGAGGGGTCCCGGCCAACAAACCAGAGTCAGCCCGAGCCAAGGCCAAG CTTGCCAAGTTCAGCATCCCCAAGCAGGCAGAGGAGAGTAGATCTGGAGAAAACAACAGCTGTGTGGAAG AAATAATTCGGGAGATGACCTGGCTTCCTCCACTTTCTGCTATTCAAGCTCCTGCCAAAGTGGAACCAAG CAAGTTCCCATTCCCAAATAAGGACTCGCAACTTGTATCCTCTGGACACAGTAATCCAAAGAAAGCTGAT GCGGAGCCAGGGAGTCCAGACAATGGCGCATCGAACACTTCAACTCTGGAAGACGACCTTAAGCTAAGCA GTGATGACGAAGAGGGTGAGCAGCAGGCAGCCCAGAGAACAGCTCTCCGCGCTCTAGCTGACAGCTCCGT GGTTCAGCAGACCAACTGTAGAGGTTCGGCGCCATCCAGCAAGGGCGGCGGCAGCAGCAGCAGCAGCGGC GGCAGCAGCTCCTCCAGCGACTCAGAGAGCACTTCCGGCTCCGACTCCGAGACGGAGAGCAGCTCCAGCT CCAGCGAGAGCGAGGGCAGCAAGCCTCCGCACTGCTCCAGCCCCGAGGCCGAACCTGCGTCCTCTAACAA GTGGCAGCTGGATAAATGGCTAAACAAAGTTAATCCTCACAAGCCGCCAATTCTGATCCAAAATGAAAGC CACGGCCCGGAAAGGAATCAATACTACACGCCGCCGGTGAAAGATGAAGGGCAAGACTGCGGGAAACTTC CTGAGATCTGCCAAGCCAGCCTGAGGGACAAGGAGCTCAAGACCACCTGCAAGGAGGAGCAGAGGCCGAG GACCGCAAACAAGGCCCCTGGCAGCAAAAGTGTAAAGCAGAAGTCCCCACCCGCTGCTGTGGCTGTGACC GCAGCTGCCCTCCCGCCCGCGGTGCCCAGCGCACCCACGGAGAGCGCGCCTGCGCCCACCCGCAGGTCAG CAGGCAAGAAGCCCACCCGGCGCACCGAGAGGACCTCAGCCGGGGACGGTGCCAACTGCCACCGGCCTGA GGAGCCAGTGGCCCCAGACACGCTGGGGGCGAGCGTGGTGGGACCCCTAGAGCCCCCCAAAACCAGACCC TGCGGTAACAACAGGACAGGCCACCGCAAGGAGCTGCGCTCCTCCGTGACCTGTGAGAAGCGGCGCACGA GGGGGCTGAGCAGAATCGTCCCCAAATCCAAGGAGTTTATTGAAACAGAATCTTCGTCTTCCTCTTCCTC CTCGGACTCAGACCTGGAGTCAGAGCAAGAGGAGTACGTCCTGTCCAAGGCTCCGACCACCACTGGGAGC GAGCAGCGGCTGAAGGAGGCCGCCAGCAGCAACAACAACAGCAACAGCAACAGCAGCACCTCCCGGGCTT CTGTGGGCTCCATCAACGCCAGGACCACCAGTGACATTGCCAAGGAGCTAGAGGAACAGTTCTACACGCT GGTCCCCTTTGGCAGGAATGAACTACTATCTCCGCTAAAGGACAGCGATGAGGTCAGGTCTCTCTGGGTC AAAATTGACCTGACCCTCCTGTCCAGGATCCCAGAACACCTGTCCCAGGAGCCTGGGGTTTTGAGTGCTC CTTCGGCCAAGGACACTGATAGTGCCCCAGCCAGTCATGCATTAGACGCTCCTGCTGAAAAGACTTTGCC AAAATCCAAGAGGAAACGCAAGTGCGACAACGAAGATGACTACAGGGAGATAAAGAAGGTCCAGGGAAGG AAGGAGAGTGCCTCACGCCTCGCCGCCTCCACTAATAACACCTTGTCCGGCAATCACTGCAATGTGAACG TCAACAGCTTGGCAATACCAATAAACAAAAATGAAAAGATGCTCCGGTCGCCCACTTCGCCACTCTCAGA CACATGTAAACACAAATACGCCAGCGAGGACCTGACTTCTTCCAGCCGACCTCACGGCAACGGGTTGTTG ACTTCAGCGTCTTCCAACAAAGAGCCAAAGGCTGAGAGTCAGCTGCAGACGATTGCTGGGGACCTCACGA AGGCATCTCACAACAGTTCTGAAAATGGCACCCTCCATAGCAAGTCACGGCCCCAGACGGAGCCATGGTC TCCAGGTTCCAATGGCCACAGGGACTGCAAGAGACAGAAACTGATCTTCGATGACATGCCTCGAAGTGCA GATTATTTTATGCGAGAAGCCAAGCGGATGAAACATAAAGCAGACGCCATGGTGGAGAAGTTCGGGAAGG CCCTGAACTATGCCGAAGCTGCCTTGTCCTTCATCGAGTGCGGAAACGCAATGGAACAAGGGCCAATGGA ATCCAAGTCTCCCTACACCATGTACTCGGAGACAGTGGAGCTCATCAGGTATGCTATGAGACTAAAAACC CACTCAGGCCCCAATGCCACACCAGAGGACAAACAGTTAGCTGCGCTTTGTTACCGATGCCTGGCTCTCC TATACTGGCGGATGTTTCGACTCAAAAGAGACCATGCTGTAAAATATTCAAAAGCACTTATCGACTATTT CAAGAATTCATCGAAAGCTGCCCAAGCCCCATCCCCATGGGGGTCCAGTGGCAAGAGCACTGGAAGCCCA TCCCCCATGTCTCCCAACCCTTCTCCCGCCAGCTCCGTGGGGTCTCAGGGCAGCCTCTCCAGCTCCAGCG GCCTGTCCCCATCGACCATCGTCAGCATCCCCCAGCGCATTCACCAGATGGCGGCCAATCACGTCAGCAT CACCAACAGCATCCTCCACAGCTATGACTACTGGGAGATGGCTGATAACCTGGCCAAGGAAAACAGAGAG TTCTTCAATGACCTGGATTTGCTCATGGGACCTGTCACCCTGCACAGCAGCATGGAACACCTGGTCCAGT ACTCCCAGCAAGGCCTGCATTGGCTTCGGAGCAGCGTACACCTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231699 representing NM_001290814 Red=Cloning site Green=Tags(s) MDSFDLALLQEWDLESLCVYEPDRNALRRKERERRSQETQQDSGSFNSGYSLFSEPYKTNKGDELSNRIQ NTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQTPVNKIDEHFGAESRAQPQPSTVCSTASSTPAAVPVQQ GKRGAMGWQKAGHPPSDGQQRAAQQGSLRTLLGDGVGRQQTRAKQVCNMETGLQTQERPPAMAAKHGGSG HCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETAVHCTAYRGVPANKPESARAKAK LAKFSIPKQAEESRSGENNSCVEEIIREMTWLPPLSAIQAPAKVEPSKFPFPNKDSQLVSSGHSNPKKAD AEPGSPDNGASNTSTLEDDLKLSSDDEEGEQQAAQRTALRALADSSVVQQTNCRGSAPSSKGGGSSSSSG GSSSSSDSESTSGSDSETESSSSSSESEGSKPPHCSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNES HGPERNQYYTPPVKDEGQDCGKLPEICQASLRDKELKTTCKEEQRPRTANKAPGSKSVKQKSPPAAVAVT AAALPPAVPSAPTESAPAPTRRSAGKKPTRRTERTSAGDGANCHRPEEPVAPDTLGASVVGPLEPPKTRP CGNNRTGHRKELRSSVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEEYVLSKAPTTTGS EQRLKEAASSNNNSNSNSSTSRASVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWV KIDLTLLSRIPEHLSQEPGVLSAPSAKDTDSAPASHALDAPAEKTLPKSKRKRKCDNEDDYREIKKVQGR KESASRLAASTNNTLSGNHCNVNVNSLAIPINKNEKMLRSPTSPLSDTCKHKYASEDLTSSSRPHGNGLL TSASSNKEPKAESQLQTIAGDLTKASHNSSENGTLHSKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSA DYFMREAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKT HSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGSSGKSTGSP SPMSPNPSPASSVGSQGSLSSSSGLSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENRE FFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRSSVHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290814
ORF Size	3687 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290814.1, NP_001277743.1
RefSeq Size	7251 bp
RefSeq ORF	3690 bp
Locus ID	16764
MW	133.8 kDa

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