Adamts13 (NM_001290463) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADAM-TS13; ADAMTS-13; Gm710; vWF-CP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231787 representing NM_001290463 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGCTTTGCCTGTGGTTGACGTGCCAGCCTTGTTATGCTGTCAGTGTCAGAGGAATCCTCACTG GTGCCATCTTCATTCTGGGCTGCTGGGGGCTCTCTGACTTCCAGAAGAGTCTTCTTCAAGATCTGGAGCC CAAGGATGTGTCTTCTTACTTTGGCCACCATGCTGCTCCATTCACAGGCCATCCTCCCTCTCACCTCCAG AGACTGAGACGGAGAAGGACTTTGGAGGACATTCTGCACCTGGAACTCCTGGTAGCTGTGGGCCCCGATG TTTCCCGGGCTCATCAGGAGGACACAGAACGCTACGTGCTCACTAATCTCAATATCGGGTCAGAACTGTT GAGAAACCCATCCCTGGGAGTCCAGTTCCAGGTGCACCTGGTGAAGCTAATCACCCTCTCTGACTCAGAG AGTACTCCGAATATCACGGCCAACATCACCTCATCCTTGATGAGCGTCTGCGAGTGGAGCCAGACGATCA ACCCCCACGATGACAGGGATCCAAGTCACGCTGACCTGATTCTCTATATCACCAGGTTTGACCTGGAGTT GCCTGATGGCAACCAGCAGGTTCGGGGTGTCACCCAGCTGGGAGGTGCCTGCTCCCTTTCCTGGAGTTGC CTTATCACTGAGGATACTGGCTTTGACCTGGGGGTCACCATCGCCCATGAGATTGGGCACAGCTTCGGGC TGGACCATGATGGTGCTCCAGGTAGTGGCAGCACCTGCAAGGCCAGTGGCCACGTGATGGCGGCTGATGG CGCAACACCTACTGGAGGGACCCTGGAGTGGTCTGCCTGCAGCCAAAGGCAGTTGCAGCACCTACTCAGC ACAGGGCAAATGCACTGCTTCCAGGACCCACCTGGGCTGCAGTCAGGACTTACACGGCACCAGCTGATGG CACAGCCTGGCCTCTACTACAGTGCAGATGATCAGTGCCGTGTGGCTTTCGGTTCTGGGGCTGTCGCCTG CACCTTCTCCAGGGAGGGTCTGGATGTATGCCAGGCCCTGTCCTGCCACACAGACCCCTTGGACCAAAGC AGCTGCAGCCGCCTCCTTGTTCCTCTCCTGGATGGGACAGAATGTGGTGTGGAGAAGTGGTGCTCCAAGG CTCGCTGTCGCTCCCTAGCTGAGCTGGCTCCTGTGGCTGCAGTACATGGACACTGGTCTAGCTGGGGCCC CCATAGTCCCTGCTCCCGATCCTGTGGAGGAGGTGTGATTACCAGGAGGCGGTGGTGCAACAACCCCAGG CCTGCATTTGGGGGACGTGCATGTGTGGGTGAAGACCTCCAGGCTAAGATGTGCAACACGCAGGCTTGTG AGAAGACTCAGCTGGAGTTCATGTCCGAGCAGTGTGCCCAGACAGACAGACAACCACTGCAACTTTCCCA AGGCACTGCCTCCTTCTACCACTGGGATGCTGCTGTGCAGTATAGTCAAGGAGATACCCTGTGCAGACAC ATGTGCTGGGCTGTTGGAGAAAGCTTCATTGTCAGCCGTGGGGACAGGTTCCTAGATGGGACCCGTTGTG TGCCAAGTGGTCCTCAGGATGATGGGACCCTAAGCCTCTGTTTGTTGGGCAGCTGCAGGACCTTTGGCTG TGATGGCAGGATGGACTCCCAGAAGGTTTGGGATGCGTGCCAGGTGTGTGGAGGAGACAACAGCACCTGC AGCTCACGGAATGGTTCTTTCACAGCTGGGAGAGCCAGAGAATATGTCACGTTCCTGATTGTTACTCCCA ACATGACCAACGCACACATTGTCAACCGCAGGCCTCTCTTCACACACTTGGCGGTGAGGATCCAGGGCCA CTACATTGTGGCAGGGAAGACTAGCATCTCACCCAACACCACCTACCCTTCCCTTCTGGAGGACTACCGT GTGGAATACAGAGTGACTCTCACTGAGGACCAGCTGCCCCACTTAGAGGAGATTCACATCCGGGGACCCG TCCGGGATGACATTGAGATTCAGGTGTACAGACGATATGGAGGAGAATATGGGGATCTTACACACCCAGA CATCACCTTTTCCTACTTTCAACTGAAGCAGCAGGCAGCCTGGGTATGGACCGCTAAGCGTGGACCCTGC TCAGTGAGCTGTGGGGCAGGGCTGCGCTGGGTGACCTACAGCTGCCAGGATCAAGCTCAAGACAAGTGGG TAAAGAACGCCCAGTGCCAAGGGAGCCCACAGCCACCTGCATGGCAAGAGCCTTGTGTCTCTGCCCCCTG CTCCCCATATTGGGTAGCTGGGGACTTCAGCCCATGTAGCGTGTCTTGTGGCGGGGGCCTTCGGGAGCGG TCACTGCGCTGTGTAGAGACCCAAGATGGCTTCTTAAAGACACTGCCACCTGCCCGGTGCAGAGCAGTAG CCCAGCAGCCAGCAGCAGAAGTGGAAAACTGCAACTCCCAGCCCTGTCCCACCAGGGCGGGTGACCCGGA GAAGCCAGAAACTGCAGGCCCCTGCCGCACCGACGAGATGTCAGCTATGCTGGAGCCCTGCTCCAGGAGC CTGTGTTCTCCAGGCTTGGGTCAGGTGGACAACACCATGTCTCTGGGAGAGGAGGCTCCATCCCCGGTGG GCAGTGACAAGCCAGGGGCTCAGGCTGAGCATGTGTGGACCCCTCTGGTGGGGCTGTGCTCCATCTCTTG TGGGAGAGGTCTGAAGGAACTGTATTTCCTGTGCATGGATTCTGTCCTCAAAATGCCTGTCCAGGAAGAG CTATGCGGCTTGGCTAGTAAGCCCCCAAGCCGGTGGGAGGTCTGCAGGGCTCGCCCCTGTCCTGCTCGGT GGGAGACTCAAGTCTTGGCACCGTGCCCGGTGACCTGTGGTGGGGGGCGAGTGCCACTGTCTGTTCGTTG TGTGCAGCTAGACCGTGGCCACCCGATATCTGTACCTCACTCCAAGTGCTCGCCAGTGCCTAAGCCAGGC TCCTTCGAGGACTGCAGCCCTGAGCCTTGTCCTGCTAGATGGAAAGTCCTGTCCCTTGGCCCATGCTCAG CCAGCTGTGGCCTTGGCACTGCCACACAAATGGTGGCCTGCATGCAGCTTGACCAGGGCCATGACAATGA GGTGAATGAAACTTTCTGTAAGGCTCTAGTGCGGCCTCAGGCCAGTGTCCCCTGCCTCATTGCTGACTGC GCATTCCGGTGGCACATCAGCGCCTGGACAGAGTGCTCTGTCTCCTGTGGGGACGGTATTCAGCGCCGGC ATGACACCTGCCTTGGACCCCAGGCCCAAGTGCCTGTGCCAGCCAATTTCTGCCAGCACCTGCCCAAGCC AATGACCGTGAGAGGCTGCTGGGCTGGACCCTGTGCAGGGCAGGAGACCTCCAGCTCACTGCCTCACAAG GAAGCTACTCTTCCCAGTCAGACCCAGGCTGCTGCTACTGTTGCTTCTCTGCAGTGGTCCCAGCCCCGAG CCCGGACCCCCACCCTTTTCTCAGCCTCCCAGTCTCTGGGGCTCCAGGAAAACCTGGAAGAGCATGGTGC CTGTGGAAGGCAGTACCTGGAGCCCACAGGAACCATTCACATGCGAGATCAAGGACGGCTAGACTGTGTG GTGGCCATTGGGAGACCTCTAGGTGAAGTGGTGACCCTACAAATCCTTGAGAGCTCCCTCAAGTGTAGTG CAGGGGAGCAACTGCTGCTCTGGGGTAGGTTCACATGGAGGAAGACATGCAGGAAGATGCCTGGCATGAC TTTCAGCACCAAAACCAACACAGTGGTGGTGAAGCAGCATCGTGTGCTGCCAGGGGGTGGAGTGCTGCTA CGGTACTGGAGTCAACCTGCCCCGGGGACCTTCTACAAAGAATGTGACAGGCAGCTCTTTGGACCTCGGG GTGAAATTGTGAGTCCCTCACTGAGCCCAGACGGGAGGAAGGCAGGGACCTGCAGGGTCTTCATCAGTGT GGCTCCACAGGCCCGCATTGCCATCCGCGCCCTGGCCAGTGATATGGGCACTGCTTCTGAGGGGACCAAT GCCAACTATGTCTCGATCAGGGACATCCACAGTCTGAGGACCACAACATTTTGGGGGCAGCAGGTGCTCT ACTGGGAGTCCGAAGGCAGCGAGGCTGAACTGGAGTTTAGCCCTGGCTTCCTGGAGGCACATGCCAGTCT CCAGGGCGAGTATTGGACCATCTCACCTAGGACATCGGAACAGGACGACAGCCTGGCTCTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231787 representing NM_001290463 Red=Cloning site Green=Tags(s) MSQLCLWLTCQPCYAVSVRGILTGAIFILGCWGLSDFQKSLLQDLEPKDVSSYFGHHAAPFTGHPPSHLQ RLRRRRTLEDILHLELLVAVGPDVSRAHQEDTERYVLTNLNIGSELLRNPSLGVQFQVHLVKLITLSDSE STPNITANITSSLMSVCEWSQTINPHDDRDPSHADLILYITRFDLELPDGNQQVRGVTQLGGACSLSWSC LITEDTGFDLGVTIAHEIGHSFGLDHDGAPGSGSTCKASGHVMAADGATPTGGTLEWSACSQRQLQHLLS TGQMHCFQDPPGLQSGLTRHQLMAQPGLYYSADDQCRVAFGSGAVACTFSREGLDVCQALSCHTDPLDQS SCSRLLVPLLDGTECGVEKWCSKARCRSLAELAPVAAVHGHWSSWGPHSPCSRSCGGGVITRRRWCNNPR PAFGGRACVGEDLQAKMCNTQACEKTQLEFMSEQCAQTDRQPLQLSQGTASFYHWDAAVQYSQGDTLCRH MCWAVGESFIVSRGDRFLDGTRCVPSGPQDDGTLSLCLLGSCRTFGCDGRMDSQKVWDACQVCGGDNSTC SSRNGSFTAGRAREYVTFLIVTPNMTNAHIVNRRPLFTHLAVRIQGHYIVAGKTSISPNTTYPSLLEDYR VEYRVTLTEDQLPHLEEIHIRGPVRDDIEIQVYRRYGGEYGDLTHPDITFSYFQLKQQAAWVWTAKRGPC SVSCGAGLRWVTYSCQDQAQDKWVKNAQCQGSPQPPAWQEPCVSAPCSPYWVAGDFSPCSVSCGGGLRER SLRCVETQDGFLKTLPPARCRAVAQQPAAEVENCNSQPCPTRAGDPEKPETAGPCRTDEMSAMLEPCSRS LCSPGLGQVDNTMSLGEEAPSPVGSDKPGAQAEHVWTPLVGLCSISCGRGLKELYFLCMDSVLKMPVQEE LCGLASKPPSRWEVCRARPCPARWETQVLAPCPVTCGGGRVPLSVRCVQLDRGHPISVPHSKCSPVPKPG SFEDCSPEPCPARWKVLSLGPCSASCGLGTATQMVACMQLDQGHDNEVNETFCKALVRPQASVPCLIADC AFRWHISAWTECSVSCGDGIQRRHDTCLGPQAQVPVPANFCQHLPKPMTVRGCWAGPCAGQETSSSLPHK EATLPSQTQAAATVASLQWSQPRARTPTLFSASQSLGLQENLEEHGACGRQYLEPTGTIHMRDQGRLDCV VAIGRPLGEVVTLQILESSLKCSAGEQLLLWGRFTWRKTCRKMPGMTFSTKTNTVVVKQHRVLPGGGVLL RYWSQPAPGTFYKECDRQLFGPRGEIVSPSLSPDGRKAGTCRVFISVAPQARIAIRALASDMGTASEGTN ANYVSIRDIHSLRTTTFWGQQVLYWESEGSEAELEFSPGFLEAHASLQGEYWTISPRTSEQDDSLALS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290463
ORF Size	4194 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290463.1, NP_001277392.1
RefSeq Size	4496 bp
RefSeq ORF	4197 bp
Locus ID	279028
UniProt ID	Q769J6
MW	152.9 kDa
Gene Summary	This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. In certain mouse strains (C57BL/6, for example) an intracisternal A-type particle (IAP) retrotransposon sequence is located in the intron 23 that causes an alternate splicing event resulting in a shorter transcript variants encoding shorter isoforms. The encoded preproprotein undergoes proteolytic processing to generate an active enzyme that cleaves von Willebrand factor (VWF) in circulating blood. [provided by RefSeq, Jul 2016]

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