Taf1 (NM_001290729) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU015687; B430306D02Rik; Ccg-1; Ccg1; KAT4; N-TAF1; p250; Taf2a; TAFII250
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231893 representing NM_001290729 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGGTGGGCTGGGCTGCTGCAGGACAAGGGTGGCGGTAGCCCTTCCGTCGTCATGTCCGACA CAGACAGCGACGAAGAGTCGGCTGGTGGAGGCCCATTTTCTCTAACTGGGTTCCTGTTCGGCAACATCAA TGGAGCAGGGCAGTTGGAAGGCGAAAGCGTCTTGGATGACGAGTGCAAGAAGCATCTTGCGGGCTTGGGG GCTTTGGGTCTTGGCAGCCTGATCACTGAACTCACAGCAAATGAAGAATTGTCCGGGAGTGATGGTGCCC TGGTAAATGATGAAGGATGGATTAGGAGTAGAGAGGATGCTGTGGACTATTCTGACATCAACGAGGTGGC AGAAGATGAAAGTAGAAGATACCAGCAGACAATGGGAAGCTTGCAACCCCTTTGCCACACAGATTATGAT GAAGATGACTATGATGCTGATTGTGAAGATATTGATTGCAAATTGATGCCTCCTCCACCTCCACCCCCAG GACCACTGAAGAAAGAGAAGGACCAAGATGATATTACTGGTGTGTCTGAAGATGGAGAAGGCATCATCTT GCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCTGACTCAGAA TCTGAGATGGGACCTCAGGACGCAGCACAGTCAGAATCTAAGGATGGACAGCTGACCCTACCATTGGCTG GGATTATGCAGCATGATGCCACGAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTAGGCCTGG AAAGGTGTTACGCTTTTTACGGCTTTTTGGACCGGGGAAGAATGTTCCATCTGTTTGGAGGAGTGCTCGG AGAAAGAGGAAAAAGAAGCACCGTGAGCTTATACAGGAAGGGCAGGTCCAGGAAGAGGAGTGCTCAGTGG AATTAGAAGTCAACCAGAAATCTCTGTGGAACTATGACTATGCTCCTCCTCCACTTCCAGACCAGTGTCT CTCTGATGATGAAATCACAATGATGGCTCCTGTGGAGTCCAAGTTTTCCCAGTCAACTGGAGACACAGAT AAAGTAATGGATACCAAGCCAAGAGTGGCCGAATGGCGTTATGGACCTGCCCGGCTGTGGTATGATATGC TAGGTGTTCCTGAAGATGGCAGTGGCTTTGATTATGGCTTCAAAATGAAGAAGACTGAACATGAATCTAC AATAAAATGCAACATAATGAAGAAATTAAGGAAACTTGAGGAAAACAGTGGCGTCGATCTTCTGGCAGAT GAAAACTTCCTAATGGTGACACAGCTGCATTGGGAAGATGATATCATTTGGGATGGGGAGGATGTCAAAC ACAAAGGAACAAAACCTCAGCGTGCGAGTTTGGCAGGCTGGCTTCCTTCCAGTATGACTAGGAATGCCAT GGCCTACAATGTGCAGCAAGGTTTTACAGCTACATTGGATGATGATAAACCTTGGTACTCCATTTTCCCC ATTGATAATGAAGATCTGGTATATGGACGTTGGGAGGACAATATCATTTGGGATGCTCAGAACATGCCTA GAATTTTGGAGCCTCCTGTTTTGACACTTGATCCCAATGATGAGAACTTGATTTTGGAGATCCCTGATGA GAAGGAAGAAGCTACTTCTAATTCTCCTTCTAAGGAGAATAAGAAAGAATCATCTCTGAAGAAGAGTCGA ATTCTCTTAGGAAAAACAGGAGTCATCAAGGAAGAGCCACAGCAGAACATGTCTCAGCCAGAAGTGAAAG ATCCATGGAATCTCTCCAATGATGAGTATTACTATCCCAAGCAACAGGGCCTTCGAGGTACTTTTGGAGG AAATATTATCCAGCACTCAATTCCAGCTGTAGAATTACGGCAGCCCTTCTTTCCTACCCACATGGGACCC ATCAAACTTCGGCAGTTCCATCGCCCACCTCTAAAAAAGTACTCCTTTGGGGCACTATCTCAGCCAGGTC CACACTCAGTTCAGCCTTTGCTGAAGCACATCAAAAAGAAGGCAAAGATGAGAGAACAAGAGAGGCAAGC CTCGGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGATCTCACAGGCAAAGATGGAGACCTTATT CTTGCTGAATACAGTGAGGAAAATGGACCATTAATGATGCAAGTTGGCATGGCAACCAAGATAAAAAACT ACTATAAGCGGAAACCTGGAAAAGATCCTGGAGCCCCAGATTGCAAATACGGAGAAACTGTTTATTGCCA TACATCGCCTTTCCTGGGTTCTCTTCATCCTGGCCAGTTACTGCAGGCATTTGAGAACAACCTTTTTCGT GCTCCAATTTATCTTCATAAGATGCCAGAATCTGACTTCCTGATCATTCGAACAAGGCAGGGTTACTTTA TTCGAGAATTAGTGGATATTTTTGTTGTTGGCCAGCAGTGTCCCTTATTTGAAGTTCCTGGTCCTAACTC CAAAAGGGCCAATACACACATCCGAGATTTTCTTCAGGTTTTTATTTACCGGCTCTTTTGGAAGAGTAAA GATAGGCCACGGAGGATACGGATGGAAGATATAAAGAAAGCCTTTCCTTCACATTCAGAAAGCAGCATCC GGAAGAGGCTAAAGCTCTGTGCCGACTTCAAACGTACAGGGATGGATTCAAACTGGTGGGTGCTGAAGTC TGATTTTCGTTTACCAACGGAAGAGGAGATCCGAGCTATGGTATCCCCAGAACAGTGCTGTGCTTATTAT AGCATGATAGCTGCTGAACAGAGACTAAAGGATGCTGGCTATGGAGAGAAGTCCTTTTTTGCTCCTGAGG AAGAAAATGAAGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGTACTGCTCCTTGGAATACTACAAG GGCTTTCATTGCTGCCATGAAGGGCAAATGTCTCCTGGAGGTGACTGGAGTAGCAGATCCTACAGGCTGT GGTGAAGGGTTCTCCTATGTGAAGATTCCAAACAAACCGACACAACAGAAGGATGATAAAGAGCCTCAGC CAGTGAAAAAGACAGTCACAGGAACAGATGCAGACCTTCGTCGCCTGTCTCTGAAAAATGCCAAGCAGCT TCTTCGTAAATTTGGTGTGCCTGAGGAGGAGATTAAAAAGTTGTCCCGCTGGGAAGTTATTGATGTGGTA CGTACAATGTCAACAGAACAGGCTCGCTCTGGAGAGGGGCCCATGAGTAAATTTGCTCGTGGATCAAGGT TTTCAGTGGCTGAGCATCAAGAACGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGT TTTGTCATCAACAGAGGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGACAGTGACTTTGAAGAA ATGGGAAAGAATATTGAGAATATGCTGCAGAACAAGAAAACAAGTTCTCAGCTGTCTCGGGAGCGGGAGG AGCAGGAACGGAAGGAACTCCAGCGGATGCTACTGGCAGCAGGCTCAGCAGCAGCAGGAAACAATCACAG AGATGATGACACAGCTTCAGTGACCAGCCTTAACTCTTCTGCCACTGGCCGCTGTCTCAAGATTTATCGA ACATTTCGAGATGAGGAGGGGAAGGAATATGTTCGTTGTGAGACAGTCCGAAAAGCAACTGTTATTGATG CTTATGTACGCATACGGACCACAAAAGATGAAGAGTTCATTCGAAAATTTGCCCTTTTTGATGAGCAACA CCGAGAAGAGATGAGAAAAGAACGGCGGAGGATTCAAGAGCAATTGAGGAGACTTAAACGGAACCAGGAA AAGGAAAAGCTAAAGGGGCCTCCTGAGAAAAAGCCCAAAAAAATGAAGGAACGGCCTGACTTAAAATTGA AATGTGGAGCATGTGGTGCCATCGGGCACATGAGGACAAATAAATTCTGCCCCCTTTATTATCAAACAAA TGCTCCACCTTCTAACCCTGTTGCTATGACCGAGGAACAAGAGGAAGAGCTGGAAAAGACAGTCATTCAT AATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAGATCGTTTTGGGCAAACAGCTAATTGAGAGTG CAGATGAAGTTCGCAGAAAATCTCTGGTCCTGAAGTTCCCTAAACAACAACTTCCCCCCAAGAAAAAACG ACGGGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAATCCATCCACCGGCGCCGGACA GACCCGATGGTGACACTGTCTTCTATCTTGGAGTCTATTATCAATGACATGAGAGATCTTCCAAATACAT ACCCTTTCCATACTCCAGTTAATGCAAAGGTTGTAAAGGATTACTATAAAATCATCACTCGACCAATGGA TCTACAGACACTCCGTGAAAACGTGCGCAAACGCCTTTATCCGTCTCGGGAAGAATTCAGAGAACATTTG GAGCTAATTGTGAAAAACAGTGCAACTTATAATGGACCAAAGCACTCACTGACTCAGATTTCTCAATCCA TGCTGGATCTCTGCGATGAAAAACTCAAAGAGAAAGAGGATAAGTTGGCACGTTTAGAGAAAGCTATCAA CCCCTTGCTTGACGATGATGATCAAGTAGCCTTTTCTTTTATTCTGGATAACATTGTCACTCAGAAAATG ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTATTACAAAG TGATTGTCAGTCCAATGGATTTGGAGACTATACGTAAGAATATCTCCAAGCACAAATACCAGAGTCGAGA GAGCTTTCTAGACGACGTAAACCTGATTCTGGCCAACAGTGTTAAGTACAATGGGCCTGAGAGTCAGTAT ACTAAGACTGCTCAGGAGATTGTGAACGTTTGTCACCAGACATTAACTGAGTATGATGAACATTTGACTC AACTTGAGAAAGATATTTGTACAGCTAAAGAAGCAGCTCTGGAGGAAGCAGAATTAGAAAGTCTGGACCC AATGACTCCAGGGCCATATACACCTCAGGCTAAGCCTCCTGATTTGTATGATAACAACACATCCCTCAGT GTTTCTCGAGATGCTTCTGTATATCAAGATGAGAGCAATCTGTCTGTCTTGGATATTCCCAGTGCTACTT CAGAAAAGCAGCTAACACAGGAAGGTGGAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTGT ACAACAGCCCCAAGCCAGTGTCTTGTATGAGGATTTGCTTATGTCTGAAGGCGAAGATGATGAGGAAGAT GCTGGGAGTGACGAAGAAGGAGATAACCCTTTCTTTGCTATTCAGCTAAGTGAAAGTGGAAGTGACTCTG ATGTGGAATCTGGCAGTTTAAGACCCAAACAGCCCCGTGTGCTTCAAGAGAATACAAGGATGGGCATGGA AAATGAAGAAAGCATGATGTCCTATGAGGGAGATGGTGGGGACGCTTCCCGTGGTTTGGAGGATAGCAAC ATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCCAACACCCAGGATACCAGCTTCAGCAGCATCG GTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAAGAGCAGCGCTCTGGGCCCAGCGTACTAAGCCA GGTCCACCTCTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCCGGAGACACTGATTTGGAC TCTGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231893 representing NM_001290729 Red=Cloning site Green=Tags(s) MGPGWAGLLQDKGGGSPSVVMSDTDSDEESAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLG ALGLGSLITELTANEELSGSDGALVNDEGWIRSREDAVDYSDINEVAEDESRRYQQTMGSLQPLCHTDYD EDDYDADCEDIDCKLMPPPPPPPGPLKKEKDQDDITGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSE SEMGPQDAAQSESKDGQLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSAR RKRKKKHRELIQEGQVQEEECSVELEVNQKSLWNYDYAPPPLPDQCLSDDEITMMAPVESKFSQSTGDTD KVMDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKMKKTEHESTIKCNIMKKLRKLEENSGVDLLAD ENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFTATLDDDKPWYSIFP IDNEDLVYGRWEDNIIWDAQNMPRILEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSR ILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGP IKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLI LAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFR APIYLHKMPESDFLIIRTRQGYFIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSK DRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYY SMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGC GEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVV RTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEE MGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAAAGNNHRDDDTASVTSLNSSATGRCLKIYR TFRDEEGKEYVRCETVRKATVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQE KEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIH NDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRT DPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHL ELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKM MAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQY TKTAQEIVNVCHQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDNNTSLS VSRDASVYQDESNLSVLDIPSATSEKQLTQEGGDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEED AGSDEEGDNPFFAIQLSESGSDSDVESGSLRPKQPRVLQENTRMGMENEESMMSYEGDGGDASRGLEDSN ISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDTDLD SDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290729
ORF Size	5679 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290729.1, NP_001277658.1
RefSeq Size	8033 bp
RefSeq ORF	5682 bp
Locus ID	270627
UniProt ID	Q80UV9
MW	215.1 kDa
Gene Summary	Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:10438527). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4.[UniProtKB/Swiss-Prot Function]

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