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VPS28 (NM_016208) Human Tagged ORF Clone
CAT＃: RC200025
VPS28 (Myc-DDK-tagged)-Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1
ORF Plasmid: tGFP
"NM_016208" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200025 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATGGGATCCCAGCCACGCCGGGCATAGGAGCCCCTGGGAACAAGCCGGAGCTGTATGAGGAAG TGAAGTTGTACAAGAACGCCCGGGAGAGGGAGAAGTACGACAACATGGCAGAGCTGTTTGCGGTGGTGAA GACAATGCAAGCCCTGGAGAAGGCCTACATCAAGGACTGTGTCTCCCCCAGCGAGTACACTGCAGCCTGC TCCCGGCTCCTGGTCCAATACAAAGCTGCCTTCAGGCAGGTCCAGGGCTCAGAAATCAGCTCTATTGACG AATTCTGCCGCAAGTTCCGCCTGGACTGCCCGCTGGCCATGGAGCGGATCAAGGAGGACCGGCCCATCAC CATCAAGGACGACAAGGGCAACCTCAACCGCTGCATCGCAGACGTGGTCTCGCTCTTCATCACGGTCATG GACAAGCTGCGCCTGGAGATCCGCGCCATGGATGAGATCCAGCCCGACCTGCGAGAGCTGATGGAGACCA TGCACCGCATGAGCCACCTCCCACCCGACTTTGAGGGCCGCCAGACGGTCAGCCAGTGGCTGCAGACCCT GAGCGGCATGTCGGCGTCAGATGAGCTGGACGACTCACAGGTGCGTCAGATGCTGTTCGACCTGGAGTCA GCCTACAACGCCTTCAACCGCTTCCTGCATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200025 protein sequence
Red=Cloning site Green=Tags(s) MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAAC SRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVM DKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLES AYNAFNRFLHA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016208 |
| ORF Size | 663 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016208.2, NP_057292.1 |
| RefSeq Size | 994 bp |
| RefSeq ORF | 666 bp |
| Locus ID | 51160 |
| UniProt ID | Q9UK41 |
| Domains | VPS28 |
| Protein Pathways | Endocytosis |
| MW | 25.4 kDa |
| Gene Summary | This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Specific autophagy and ESCRT components participate in the unconventional secretion of CFTR
,null,
Autophagy
,PubMed ID 29969945
[VPS28]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200025L1 | Lenti ORF clone of Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200025L2 | Lenti ORF clone of Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200025L3 | Lenti ORF clone of Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200025L4 | Lenti ORF clone of Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG200025 | VPS28 (tGFP-tagged) - Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1 |
CNY 4,000.00 |
|
| SC114425 | VPS28 (untagged)-Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1 |
CNY 2,400.00 |
