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METTL9 (NM_016025) Human Tagged ORF Clone
CAT＃: RC200028
METTL9 (Myc-DDK-tagged)-Human methyltransferase like 9 (METTL9), transcript variant 1
ORF Plasmid: tGFP
"NM_016025" in other vectors (5)
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CNY 2,400.00
CNY 4,465.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-81; DREV; DREV1; PAP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200028 representing NM_016025.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTAGCGGCCCGGGTGGGCCGGCGGCGGCCGCGGGCGGCAGGAAGGAGAACCACCAGTGGTATGTG TGCAACAGAGAGAAATTATGCGAATCACTCCAGGCTGTCTTTGTTCAGAGTTACCTTGATCAAGGAACA CAGATCTTCTTAAACAACAGCATTGAGAAATCGGGCTGGCTATTTATCCAATTATATCATTCTTTTGTG TCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTATCAATGGGTTGCTAGGAAGAGGCTCAATGTTT GTGTTTTCACCAGATCAGTTTCAGAGACTGCTTAAAATTAATCCAGACTGGAAAACCCACAGACTTCTT GATTTAGGTGCTGGAGATGGAGAAGTCACAAAAATCATGAGCCCTCATTTTGAAGAAATCTATGCCACT GAGCTTTCTGAAACTATGATATGGCAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATAAATGAATGG CAGAATACGGGGTTCCAGTATGATGTCATCAGCTGCCTGAACTTGCTGGACCGCTGTGATCAGCCCCTG ACTTTGTTAAAAGATATCAGAAGTGTCTTGGAGCCAACTAGAGGCAGGGTCATCCTTGCCCTTGTCCTC CCCTTTCATCCCTATGTGGAAAACGTAGGTGGCAAGTGGGAGAAACCATCAGAAATTTTGGAAATCAAA GGACAGAACTGGGAAGAACAAGTGAATAGTCTGCCTGAAGTTTTCAGAAAAGCTGGTTTTGTTATCGAA GCTTTCACCAGACTACCATACCTGTGTGAAGGCGACATGTATAATGACTACTACGTTCTGGATGACGCT GTCTTTGTTCTCAAACCAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC200028
Blue=ORF Red=Cloning site Green=Tag(s) MTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFV SSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYAT ELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVL PFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDA VFVLKPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016025 |
| ORF Size | 954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016025.2, NP_057109.2 |
| RefSeq Size | 3267 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 51108 |
| UniProt ID | Q9H1A3 |
| Domains | DREV |
| MW | 32.4 kDa |
| Gene Summary | Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The interactome of a family of potential methyltransferases in HeLa cells
,Ignatova, VV;Jansen, PWTC;Baltissen, MP;Vermeulen, M;Schneider, R;,
Sci Rep
,PubMed ID 31036863
[METTL9]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200028L3 | Lenti ORF clone of Human methyltransferase like 9 (METTL9), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200028L4 | Lenti ORF clone of Human methyltransferase like 9 (METTL9), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200028 | METTL9 (tGFP-tagged) - Human methyltransferase like 9 (METTL9), transcript variant 1 |
CNY 4,000.00 |
|
| SC114501 | METTL9 (untagged)-Human methyltransferase like 9 (METTL9), transcript variant 1 |
CNY 7,584.00 |
|
| SC317482 | METTL9 (untagged)-Human methyltransferase like 9 (METTL9), transcript variant 1 |
CNY 6,270.00 |
